Abstract：
:The function of exocytosis during plasma membrane resealing might be to facilitate the flow of surface lipid over the disruption site and/or to add defect-spanning "patches" of internal membrane across it. Scanning-electron-microscopic visualization of large plasma membrane disruptions in sea urchin eggs is here used to distinguish between these two possibilities. Disruptions were induced by shear stress in the presence and absence of resealing-permissive levels of external Ca2+, and the eggs were fixed at various intervals thereafter for microscopic processing. In eggs fixed immediately (<1 s) after shearing in the absence of Ca2+, a condition which prevents resealing, disruption sites were filled with a uniform population of spherical vesicles (approximately 1 microm in diameter). In eggs fixed immediately after shearing at a resealing-permissive level of Ca2+, disruption sites were filled with a highly heterogeneous population of enlarged vesicles, some being more than 10 microm in diameter and many having irregular profiles and/or appearing to be joined to one another. In eggs fixed 2 s or 5 s post-shearing, the continuity of these large vesicles with one another and the surface membrane began to obscure individual vesicle identities. Single "apertures" of discontinuity over disruption sites, the predicted morphology of a flow-based resealing mechanism, were not observed at any time point (1-5 s) during the interval required for completion of resealing. These observations provide strong confirmation that "patching" of large disruptions mediates their resealing.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
McNeil PL,Baker MMdoi
10.1007/s004410000286keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
141-6issue
1eissn
0302-766Xissn
1432-0878journal_volume
304pub_type
杂志文章abstract：:Using in vitro autoradiography, binding sites of 125I-ANP (atrial natriuretic peptide) were localized in the rat placenta, visceral yolk sac, and decidua at 16, 18, and 20 days of gestation. There was diffuse binding over the labyrinthine region of the placenta and an intense binding over the decidual gland and viscer...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302744
更新日期：1993-05-01 00:00:00
abstract：:This study documents neuronal differentiation in vitro from undifferentiated precursor cells of caudalmost regenerating spinal cord of the teleost Apteronotus albifrons. At 11 days in vitro, cells from the caudalmost tip of the regenerating cord are flat and polygonal in shape, lack neuronal processes and do not stain...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00414166
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214559
更新日期：1985-01-01 00:00:00
abstract：:We have developed a primary cell culture system of antennal mechanoreceptor neurons from early-stage pupal sphinx moth Manduca sexta. Dissociated neurons from the moth antennae differentiated, grew and survived for several weeks in a conditioned culture medium. Bipolar neurons with soma diameters of 10-25 microns from...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051358
更新日期：1999-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00227032
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-1026-z
更新日期：2005-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219238
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217865
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050672
更新日期：1996-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236894
更新日期：1979-08-03 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050727
更新日期：1997-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225615
更新日期：1989-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050833
更新日期：1997-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209211
更新日期：1975-09-17 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233732
更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051340
更新日期：1999-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234755
更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258502
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0770-5
更新日期：2009-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00231997
更新日期：1975-05-27 00:00:00
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journal_title：Cell and tissue research
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更新日期：2003-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234326
更新日期：1979-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232008
更新日期：1979-05-18 00:00:00
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更新日期：2020-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218381
更新日期：1986-01-01 00:00:00
abstract：:Overexpression of type XIII collagen molecules with an 83-amino-acid residue in-frame deletion of part of the ectodomain leads to fetal lethality in Col13a1COL2del transgenic mice. We characterize here the functional disturbances in the cardiovascular system of mouse fetuses overexpressing mutant type XIII collagen. D...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0617-5
更新日期：2008-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0430-6
更新日期：2007-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217273
更新日期：1982-01-01 00:00:00
abstract：:The pituitary gland is first evident in rainbow trout two weeks before hatching. Differentiation of prolactin and ACTH cells is not marked until 3-4 days after hatching when the follicular arrangement of the prolactin cells become apparent. There is no difference in the time of development of either prolactin or ACTH ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209283
更新日期：1978-05-29 00:00:00
