Abstract：
:Quantitative studies on the population of the gonadotropic cells in the pituitary of adult male rats were performed after trypsic dissociation of the pituitary glands and immunoenzymatic staining with anti-beta-LH or anti-beta-FSH antisera. Number, area and extinction of labelled cells were measured by use of an image analyser and a cytophotometer. The gonadotrophs represent approximately 14% of the pituitary cells. The mean area of gonadotrophs is significantly larger after staining with anti-beta-LH serum than after staining with anti-beta-FSH serum. Planimetric measurement of the gonadotrophs reveals their variability in size ranging between 30 and 160 microns2. Moreover, the size distribution depends on the staining serum used: more numerous small-sized cells (less than 75 microns2) are stained with anti-beta-FSH serum than with anti-beta-LH serum, which conversely stains more numerous large-sized cells. Cytophotometric measurements indicate that immunostaining varies greatly among cells of the same size class and that the staining intensity appears to increase according to the cell size. These results emphasize the morphofunctional heterogeneity of the gonadotropic cell population.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Fellmann D,Bresson JL,Clavequin MC,Bugnon Cdoi
10.1007/BF00217273subject
Has Abstractpub_date
1982-01-01 00:00:00pages
137-44issue
1eissn
0302-766Xissn
1432-0878journal_volume
224pub_type
杂志文章abstract：:Exocytotic release of the secretory granules of the endocrine cells in the midgut of a cockroach, Periplaneta americana, was studied by means of fixation with tannic acid in combination with glutaraldehyde and osmium tetroxide. A sequence of images indicative of exocytosis suggests the following steps in this process:...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213851
更新日期：1982-01-01 00:00:00
abstract：:Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1094-1
更新日期：2011-04-01 00:00:00
abstract：:To examine the selective permeability of the nephrons of lower vertebrates, the permeability of the glomerulus in the kidney of an arctic lamprey, Entosphenus japonicus (Martens), to native anionic ferritin or cationized ferritin was studied by observing the distribution of ionized anionic groups in renal tissues. The...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226944
更新日期：1984-01-01 00:00:00
abstract：:The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225806
更新日期：1988-11-01 00:00:00
abstract：:A peptide hormone was extracted from the porcine right atrium following a bioassay for differential vasorelaxant effects on smooth muscle strips from aorta and renal and inferior mesenteric arteries. The isolation procedure included several steps of gel-permeation and ion-exchange chromatography, and high performance ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219856
更新日期：1984-01-01 00:00:00
abstract：:Although the cerebrospinal fluid-contacting neurons of the avian paraventricular organ exhibit considerable amounts of catecholamines, they show no tyrosine hydroxylase immunoreactivity. In the quail embryo, the development of these neurons has been studied using the paraformaldeyde-glutaraldeyde method for the fluore...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307970
更新日期：1995-07-01 00:00:00
abstract：:The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213841
更新日期：1988-04-01 00:00:00
abstract：:The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-act...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215699
更新日期：1982-01-01 00:00:00
abstract：:Effects of prolactin on the secretion of milk proteins have been investigated by incubating mammary tissue fragments from lactating rabbits. Within 15 min of adding the hormone to the incubation medium, cell morphology is modified: the relative volume occupied by the Golgi region is greatly increased. When prolactin i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220616
更新日期：1978-02-14 00:00:00
abstract：:This investigation provides histochemical evidence for lysosomal storage of sulfated glycosaminoglycans (GAGs) in the interstitial cells of the renal cortex and in macrophage-like cells of the renal medullary zones of rats chronically treated with the drug tilorone. This compound is known to interfere with lysosomal d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218958
更新日期：1987-12-01 00:00:00
abstract：:Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210898
更新日期：1982-01-01 00:00:00
abstract：:Histological characterization of spinal fusions in Atlantic salmon (Salmo salar) has demonstrated shape alterations of vertebral body endplates, a reduced intervertebral space, and replacement of intervertebral cells by ectopic bone. However, the significance of the notochord during the fusion process has not been add...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1069-2
更新日期：2010-12-01 00:00:00
abstract：:NdWFamide is an Aplysia cardioexcitatory tri-peptide containing D-tryptophan. To investigate the roles of this peptide, we examined the immunohistochemical distribution of NdWFamide-positive neurons in Aplysia tissues. All the ganglia of the central nervous system (CNS) contained NdWFamide-positive neurons. In particu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0707-3
更新日期：2003-04-01 00:00:00
abstract：:Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0636-2
更新日期：2008-08-01 00:00:00
abstract：:The course of the Nervus connectivus (N.c.), its branches, and synaptic connections within the frontal ganglion (FG) were investigated electron microscopically after cobalt iontophoresis of the N.c. The subsequent treatment of ultrathin sections with Timm's method was found to be very suitable for identifying the smal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235172
更新日期：1979-01-01 00:00:00
abstract：:Placing the clawed toad Xenopus laevis on a black background stimulates the melanotrope cells in the pars intermedia of the pituitary gland to release proopiomelanocortin (POMC)-derived peptides, including alpha-MSH and N-acetyl-beta-endorphin. In this study three types of secretory granules, electron-dense (approxima...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00314557
更新日期：1993-12-01 00:00:00
abstract：:Larvae of Ascidia malaca, both before and after hatching, were treated with L-thyroxine solutions. The effect of the thyroid hormone was to induce the onset of metamorphosis and then to cause the rate at which body reorganization occurred to increase. In treated larvae the resorption of the tail occurred only few hour...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00249213
更新日期：1981-01-01 00:00:00
abstract：:Biocytin, recently introduced in neuroanatomical studies, was used as a retrograde tract tracer in combination with immunofluorescence in order to analyse the neurochemical characters of some central neuronal projections to the pars intermedia in two amphibian species, the anuran Rana esculenta and the urodele Trituru...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050754
更新日期：1997-02-01 00:00:00
abstract：:The ultrastructure of adult male rat pinealocytes was studied after orchidectomy and orchidectomy followed by LH-RH administration. Castration causes an increased development of the rough endoplasmic reticulum and the Golgi apparatus as well as an increase in the number of lipid droplets and lysosomes. The changes aft...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219145
更新日期：1976-04-02 00:00:00
abstract：:The effects of thyroidectomy, adrenalectomy, and castration on the pars distalis of male Japanese quail, and of injection of LH-RH on sexually inactive females, were investigated by light and electron microscopy. Correlation between light and electron microscopy was attained by use of alternate thin and thick sections...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219152
更新日期：1975-06-09 00:00:00
abstract：:Employing electron-microscopic methods that help retain polyanionic materials, we describe the extracellular coverings of a sea urchin (Lytechinus variegatus) throughout ontogeny. The surface of the embryo is covered by a two-layered cuticle (commonly called the hyaline layer), which in turn is covered by a granular l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218028
更新日期：1985-01-01 00:00:00
abstract：:Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224731
更新日期：1989-04-01 00:00:00
abstract：:This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03379-3
更新日期：2021-01-26 00:00:00
abstract：:Rats were injected with liposomes containing iodixanol (CTP10 Injection; 100 mg iodine per kg body weight) followed by a second injection of 125I-tyramine-cellobiose-albumin microspheres. The amounts of phagocytosed and degraded labelled albumin in liver were measured. A reduced uptake and degradation of albumin micro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000229
更新日期：2000-06-01 00:00:00
abstract：:Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodríguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, la...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237724
更新日期：1979-06-08 00:00:00
abstract：:Olfactory marker protein (OMP) was first described as a protein expressed in olfactory receptor neurons (ORNs) in the nasal cavity. In particular, OMP, a small cytoplasmic protein, marks mature ORNs and is also expressed in the neurons of other nasal chemosensory systems: the vomeronasal organ, the septal organ of Mas...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03349-9
更新日期：2021-01-15 00:00:00
abstract：:Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0480-9
更新日期：2008-01-01 00:00:00
abstract：:Cyclooxygenase-2 (COX-2) is a recently discovered isoform of cyclooxygenase that is inducible by various types of inflammatory stimuli. Although this enzyme is considered to play a major role in inflammation processes by catalyzing the production of prostaglandins, the precise location, distribution, and regulation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000296
更新日期：2001-02-01 00:00:00
abstract：:The developmental mutant of Drosophila (oraJK84) is characterized by nonfunctional photoreceptor cells (R1-6), while the R7/R8 cells are normal. A fundamental question is: Does the near absence of photosensitive membranes inhibit development of the R1-6 axons and their synapses at the other end of the cell? The retina...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238298
更新日期：1983-01-01 00:00:00
abstract：:The accessory medulla with its associated pigment-dispersing hormone-immunoreactive neurons appears to be the pacemaker that controls the circadian locomotor activity rhythm of the cockroach Leucophaea maderae. To permit studies at the level of individual, identified, pacemaker neurons, we developed specific long-term...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051324
更新日期：1999-06-01 00:00:00
