Electron-microscopic studies on the presence of gap junctions between corneal fibroblasts in rabbits.

Abstract:

:Corneal fibroblasts, major cellular components of the corneal stroma, are loosely arrayed between collagen lamellae. They play an important role in the metabolic and physiological homeostasis mechanisms by which the cornea is kept transparent. This paper deals with the demonstration of the gap junctions between the corneal fibroblasts of rabbits by transmission electron microscopy of thin sections and of freeze-fracture specimens. Under the transmission electron microscope, the corneal fibroblasts are seen between the lamellae of collagen fibers of the corneal stroma. Their long cytoplasmic processes are in contact with those of neighboring fibroblasts. Typical gap junctions are found between these cytoplasmic processes. In the freeze-fracture images, intramembrane particles with a diameter of 10.3 nm form polygonal aggregates on P faces. These findings suggest that corneal fibroblasts, coupled with each other, might function synchronously through gap junctions responsible for metabolic activities essential for the maintenance of corneal transparency.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Ueda A,Nishida T,Otori T,Fujita H

doi

10.1007/BF00215533

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

473-5

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

249

pub_type

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