Ultrastructural aspects of cryofixed nerves.

Abstract:

:The ultrastructure of the optic and trigeminal nerves of the rat, cryofixed by use of a liquid nitrogen-propane jet, was examined, paying special attention to the myelin sheath and the cytoskeleton of the axoplasm. The cytoskeleton of the axoplasm is formed by a meshwork of neurofilaments and microtubules connected both to each other and also to the cell organelles and axolemma. These cross-linkers are fixed to the longitudinal neurofilaments in a helical arrangement, which could be a morphological substrate for the diverse axonal transport phenomena. The myelin sheath is formed by concentrically apposed membrane pairs, which are not fused together. The corresponding major and intraperiod lines seen using classical electron microscopy are in fact fissures that are obscured by the pattern of the selective deposition of osmium at certain sites and cannot be interpreted as specific structures. The cryofixed myelin membranes have the appearance of predominantly globular subunits arranged in an asymmetrical bilayer. The globular particles are of diverse diameter and occupy varying positions within the membrane. The tight junctions or zonulae occludentes of the myelin are formed by arrays of isolated particles, and consequently the fibril formation seems to be a result of the chemical fixation.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Meller K

doi

10.1007/BF00214541

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

289-300

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

242

pub_type

杂志文章
  • Tissue- and age-dependent expression of the bovine DEFB103 gene and protein.

    abstract::Beta-defensin 103 (DEFB103) shares little homology with 8 other members of the bovine beta-defensin family and in other species DEFB103 protein has diverse functions, including antimicrobial activity, a chemoattractant for dendritic cells, enhancing epithelial wound repair and regulating hair colour. Expression of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-015-2258-9

    authors: Mirabzadeh-Ardakani A,Solie J,Gonzalez-Cano P,Schmutz SM,Griebel PJ

    更新日期:2016-02-01 00:00:00

  • Immunocytochemical demonstration of neuropeptides and serotonin in the tapeworm Diphyllobothrium dendriticum.

    abstract::The present immunocytochemical study concerns the distribution of four neuropeptides, FMRF-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of Diphyllobothrium dendriticum. Anti-FMRF-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the CNS ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222332

    authors: Gustafsson MK,Wikgren MC,Karhi TJ,Schot LP

    更新日期:1985-01-01 00:00:00

  • Localization of the pituitary lactotropes and thyrotropes within Ambystoma gracile by histochemical and immunochemical methods. A developmental study of two populations.

    abstract::The prolactin-producing cells are the first hormone-producing cells of the pars distalis to be differentiated within Ambystoma gracile. They first appear when the larvae attain a length of approximately 3.0 cm snout to vent length (SVL). Thyrotropes are observed as the next chromophilic cells to appear, and they occur...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00223121

    authors: Eagleson GW,McKeown BA

    更新日期:1978-05-18 00:00:00

  • Reinnervation of the denervated adult spinal cord of rats by intraspinal transplants of embryonic brain stem neurons.

    abstract::Previous studies have revealed a remarkable capacity of intracerebral grafts of embryonic brain tissue to establish extensive axonal connections with denervated areas in the brains of adult rats. In the present study we have explored the possibilities of using grafts in the spinal cord to substitute for the loss of no...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216024

    authors: Nornes H,Björklund A,Stenevi U

    更新日期:1983-01-01 00:00:00

  • Allergic airway inflammation induces migration of mast cell populations into the mouse airway.

    abstract::Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-017-2597-9

    authors: Schmit D,Le DD,Heck S,Bischoff M,Tschernig T,Herr C,Beisswenger C,Kobelt P,Lepper PM,Chung KF,Bals R,Dinh QT

    更新日期:2017-08-01 00:00:00

  • Subsurface cisterns and lamellar bodies in the granule cells of the guinea-pig fascia dentata.

    abstract::Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other form...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF01258502

    authors: Kolde G,Themann H

    更新日期:1982-01-01 00:00:00

  • The distribution of GABA and glycine immunostaining in the cochlear nucleus of the mustached bat (Pteronotus parnellii).

    abstract::The distribution of neuronal elements immunoreactive for gamma-aminobutyric acid (GABA) and glycine in the cochlear nucleus of the mustached bat Pteronotus parnellii has been studied by means of the postembedding technique on serial semithin sections. Our goal has been to identify similarities and differences in the o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050773

    authors: Kemmer M,Vater M

    更新日期:1997-02-01 00:00:00

  • Dictyosome-like structures from guinea-pig testes lack galactosyltransferase, a Golgi apparatus marker.

    abstract::More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217555

    authors: Morré DJ,Matyas GR,Mollenhauer HH

    更新日期:1985-01-01 00:00:00

  • Visualization of interstitial cells of Cajal in the mouse colon by vital staining.

    abstract::Interstitial cells of Cajal (ICCs) are believed to be a major element in generating the spontaneous rhythm of the gastrointestinal tract. A prominent problem in the study of these cells has been the difficulty in observing them in intact tissues. We used the lipophilic dye DiI to stain ICCs in the submucosal-circular ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051058

    authors: Hanani M,Louzon V,Miller SM,Faussone-Pellegrini MS

    更新日期:1998-05-01 00:00:00

  • Two-color double-labeling in situ hybridization of whole-mount Hydra using RNA probes for five different Hydra neuropeptide preprohormones: evidence for colocalization.

    abstract::The freshwater polyp Hydra magnipapillata has a primitive nervous system that produces at least three distinct classes of neuropeptides: various peptides having the C-terminal sequence Arg-Phe-NH2 (the Hydra-RFamide family), Leu-Trp-NH2 (the Hydra-LWamide family), and a single peptide having the C-terminal sequence Ly...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000240

    authors: Hansen GN,Williamson M,Grimmelikhuijzen CJ

    更新日期:2000-08-01 00:00:00

  • Completion of meiosis in male zebrafish (Danio rerio) despite lack of DNA mismatch repair gene mlh1.

    abstract::Mlh1 is a member of DNA mismatch repair (MMR) machinery and is also essential for the stabilization of crossovers during the first meiotic division. Recently, we have shown that zebrafish mlh1 mutant males are completely infertile because of a block in metaphase I, whereas females are fertile but have aneuploid progen...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-007-0550-z

    authors: Leal MC,Feitsma H,Cuppen E,França LR,Schulz RW

    更新日期:2008-04-01 00:00:00

  • Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?

    abstract::The application of autologous dermal fibroblasts has been shown to improve burn wound healing. However, a major hurdle is the availability of sufficient healthy skin as a cell source. We investigated fetal dermal cells as an alternative source for cell-based therapy for skin regeneration. Human (hFF), porcine fetal (p...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-015-2293-6

    authors: Akershoek JJ,Vlig M,Talhout W,Boekema BK,Richters CD,Beelen RH,Brouwer KM,Middelkoop E,Ulrich MM

    更新日期:2016-04-01 00:00:00

  • Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation.

    abstract::This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00381893

    authors: van der Ven PF,Schaart G,Jap PH,Sengers RC,Stadhouders AM,Ramaekers FC

    更新日期:1992-10-01 00:00:00

  • Role of endocardial endothelial cells in the turnover of hyaluronan in Atlantic cod (Gadus morhua).

    abstract::The fate of the major connective tissue polysaccharide hyaluronan, as it appears after release from the matrix, was studied in the Atlantic cod by use of subcutaneous administration of hyaluronan conjugated with fluorescein isothiocyanate (FITC) and labelled with 125I. After administration, the ligand was transported ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050912

    authors: Sorensen KK,Dahl LB,Smedsrod B

    更新日期:1997-10-01 00:00:00

  • A study of TSH-synthesis of spontaneous hypertensive rats by electron microscopic morphometry and autoradiography.

    abstract::In order to compare the functional state of the anterior pituitary of spontaneously hypertensive rats (SHR) with that of nonmotensive Wistar Kyoto rats (WKR), the anterior pituitary was examined by morphometry and autoradiography at the level of electron microscopy. The relative number and the relative volume of thyro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00237005

    authors: Fujiwara T

    更新日期:1979-10-01 00:00:00

  • Activation of toll-like receptor signaling in endothelial progenitor cells dictates angiogenic potential: from hypothesis to actual state.

    abstract::Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 µg/ml, for 48 h. The cell s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03405-4

    authors: Heidarzadeh M,Avcı ÇB,Saberianpour S,Ahmadi M,Hassanpour M,Bagheri HS,Rezaie J,Talebi M,Roodbari F,Sokullu E,Darabi M,Rahbarghazi R

    更新日期:2021-01-18 00:00:00

  • The innervation of the uterus in Salamandra salamandra (L.) (Amphibia, Urodela). A morphological and biochemical study.

    abstract::The innervation of the uterus in the ovoviviparous urodele Salamandra salamandra was studied. In whole mount preparations of the thin-walled uterus of pregnant females, a dense adrenergic network was demonstrated using a modified glyoxylic acid fluorescence technique. Based on vesicle type and cytochemical reactivity ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00213797

    authors: Greven H,Schindelmeiser J,Straub H

    更新日期:1983-01-01 00:00:00

  • Production of an endothelial cell migratory signal in rat endometrium during early pregnancy.

    abstract::Rat endometrial explants were cultured in a three-dimensional collagen/endothelial cell matrix to measure angiogenic activity, as represented by migration of vascular endothelial cells towards the explants. Minimal endothelial cell migratory activity was observed with endometrial explants taken during the four-day oes...

    journal_title:Cell and tissue research

    pub_type: 历史文章,杂志文章

    doi:10.1007/BF00300706

    authors: Abberton KM,Rogers PA

    更新日期:1995-01-01 00:00:00

  • Fine structure and peroxidatic activity of rat blood monocytes.

    abstract::Rat blood monocytes contain granules of two types, primary and secondary granules, which can be distinguished on morphological grounds. Secondary granules have a halo between the limiting membrane and the matrix; primary granules have no halo and are generally more elongate than the secondary granules. In addition, th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00226665

    authors: van der Rhee HJ,de Winter CP,Daems WT

    更新日期:1977-11-30 00:00:00

  • Molecular and cellular detection of expression of vitellogenin and zona radiata protein in liver and skin of juvenile salmon (Salmo salar) exposed to nonylphenol.

    abstract::In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-007-0543-y

    authors: Arukwe A,Røe K

    更新日期:2008-03-01 00:00:00

  • Effect of alpha-fluoromethylhistidine-evoked histamine depletion on ultrastructure of endocrine cells in acid-producing mucosa of stomach in mouse, rat and hamster.

    abstract::The oxyntic mucosa of the mammalian stomach is rich in endocrine cells, such as ECL cells, A-like cells, somatostatin cells, D1/P cells and, in some species, enterochromaffin cells. The various endocrine cell types can be distinguished on the basis of their characteristic cytoplasmic granules and vesicles. The ECL cel...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050707

    authors: Andersson K,Zhao CM,Chen D,Sundler F,Hâkanson R

    更新日期:1996-12-01 00:00:00

  • Histochemical and ultrastructural study of the nervous elements in the pineal organ of the eel, Anuilla anguilla.

    abstract::The pineal organ of the eel, Anguilla anguilla was studied light-and electron-microscopically. The tissue consists predominantly of glia-like supporting cells and photoreceptor-like sensory cells, which establish synaptic contacts with pineal nerve cells. Histochemically, a positive acetylcholinesterase (AchE) reactio...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00238650

    authors: Matsuura T,Herwig HJ

    更新日期:1981-01-01 00:00:00

  • Effects of chronic exercise on the ultrastructure of the adrenocortical cells in the rat.

    abstract::Four groups of male rats were exercised for periods of 2, 4, 6, and 8 weeks with controls in each group. As a result of chronic exercise there was an increase in the width of the zona fasciculata of the adrenal cortex. Also, there was an increase in the number and size of the mitochondria, and an increase in the quant...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215882

    authors: Buuck RJ,Tharp GD,Brumbaugh JA

    更新日期:1976-05-06 00:00:00

  • Surface fine structure of the globiferous pedicellariae of the regular echinoid, Psammechinus miliaris Gmelin.

    abstract::The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00220184

    authors: Oldfield SC

    更新日期:1975-10-13 00:00:00

  • Perivascular nerves in the feline carotid rete.

    abstract::Numerous nerve fibres containing acetylcholinesterase and noradrenaline, as well as avian pancreatic polypeptide-, vasoactive intestinal peptide-, or substance P-like immunoreactivity are observed around arteries in the external carotid rete of the cat. The nerves are located in the adventitial layer close to the medi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218360

    authors: Uddman R,Edvinsson L,Malm L

    更新日期:1982-01-01 00:00:00

  • Preantral intra-ovarian oocyte release in the white-footed mouse, Peromyscus leucopus.

    abstract::Adult white-footed mice, Peromyscus leucopus, were kept either in long photoperiod (LP; LD 16:8) or in short photoperiod (SP; LD 8:16) for six weeks. Ovaries were serially sectioned and examined histologically. Preantral follicles were observed to release their oocyte into the ovarian stroma. This phenomenon is termed...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218373

    authors: Spanel-Borowski K,Petterborg LJ,Reiter RJ

    更新日期:1982-01-01 00:00:00

  • Ultrastructural immunocytochemical localization of L-glutamate decarboxylase and GABA in rat pancreatic zymogen granules.

    abstract::The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar ce...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00213841

    authors: Garry DJ,Garry MG,Sorenson RL

    更新日期:1988-04-01 00:00:00

  • Distribution of neuropeptide Y-like immunoreactivity in the brain of the bichir, Polypterus senegalus, with special regard to the terminal nerve.

    abstract::The distribution of neuropeptide Y (NPY)-like immunoreactivity in the brain of the bichir, Polypterus senegalus, was examined immunohistochemically. NPY-like immunoreactivity was distributed widely in the brain, with the highest density in the diencephalon. NPY-positive perikarya were found in various areas, including...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050874

    authors: Chiba A

    更新日期:1997-08-01 00:00:00

  • Photoreceptor membrane breakdown in the spider Dinopis: localisation of acid phosphatases.

    abstract::The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236082

    authors: Blest AD,Price GD,Maples J

    更新日期:1979-07-17 00:00:00

  • Electron-microscopic studies on the presence of gap junctions between corneal fibroblasts in rabbits.

    abstract::Corneal fibroblasts, major cellular components of the corneal stroma, are loosely arrayed between collagen lamellae. They play an important role in the metabolic and physiological homeostasis mechanisms by which the cornea is kept transparent. This paper deals with the demonstration of the gap junctions between the co...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215533

    authors: Ueda A,Nishida T,Otori T,Fujita H

    更新日期:1987-08-01 00:00:00