Abstract：
:Mlh1 is a member of DNA mismatch repair (MMR) machinery and is also essential for the stabilization of crossovers during the first meiotic division. Recently, we have shown that zebrafish mlh1 mutant males are completely infertile because of a block in metaphase I, whereas females are fertile but have aneuploid progeny. When studying fertility in males in a two-fold more inbred background, we have however observed low numbers of fertilized eggs (approximately 0.4%). Histological examination of the testis has revealed that all spermatogenic stages prior to spermatids (spermatogonia, primary spermatocytes, and secondary spermatocytes) are significantly increased in the mutant, whereas the total weight of spermatids and spermatozoa is highly decreased (1.8 mg in wild-type vs. 0.1 mg in mutants), a result clearly different from our previous study in which outbred males lack secondary spermatocytes or postmeiotic cells. Thus, a delay of both meiotic divisions occurs rather than complete arrest during meiosis I in these males. Eggs fertilized with mutant sperm develop as malformed embryos and are aneuploid making this male phenotype much more similar to that previously described in the mutant females. Therefore, crossovers are still essential for proper meiosis, but meiotic cell divisions can progress without it, suggesting that this mutant is a suitable model for studying the cellular mechanisms of completing meiosis without crossover stabilization.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Leal MC,Feitsma H,Cuppen E,França LR,Schulz RWdoi
10.1007/s00441-007-0550-zsubject
Has Abstractpub_date
2008-04-01 00:00:00pages
133-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
332pub_type
杂志文章abstract：:Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217887
更新日期：1983-01-01 00:00:00
abstract：:The central fat body of the ovoviviparous cockroach Nauphoeta cinerea was studied during the first reproductive cycle of the female by means of light microscopy, autoradiography and electron microscopy. Comparative studies in larval stages were also undertaken. The fat body of Nauphoeta contains a large amount of lipi...
journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03235-4
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03163-y
更新日期：2020-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1999-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2021-01-16 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224731
更新日期：1989-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2019-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1630-x
更新日期：2013-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2892-0
更新日期：2018-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0715-3
更新日期：2003-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221474
更新日期：1989-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218011
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1979-06-27 00:00:00
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pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219959
更新日期：1975-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1998-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1978-10-30 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300691
更新日期：1995-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1987-12-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1988-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1993-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222373
更新日期：1983-01-01 00:00:00
