Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation.

Abstract:

:This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and structural organization of the intermediate filament proteins desmin and vimentin as well as the sarcomeric constituents alpha-actin, alpha-actinin, nebulin, myosin and especially titin during myofibrillogenesis in vitro, were studied by means of indirect immunofluorescence assays. The proliferating myoblasts contained both desmin and vimentin, alpha-actinin and the filamentous form of actin. Shortly after the change of medium, expression of titin, sarcomeric myosin and skeletal muscle alpha-actin was found in mononuclear cells in a diffuse, filamentous (titin, myosin, alpha-actin) or punctate (titin, myosin) pattern. Four to 10 days after the medium change, mature myotubes showed desmin, titin, alpha-actinin, nebulin, sarcomeric myosin and actin cross-striations, while vimentin was no longer detected. We conclude that human skeletal muscle cell cultures are an appropriate model system to study the molecular basis of myofibrillogenesis. Especially the presence of desmin in a striated fashion points to a high degree of maturation of the muscle cell cultures.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

van der Ven PF,Schaart G,Jap PH,Sengers RC,Stadhouders AM,Ramaekers FC

doi

10.1007/BF00381893

keywords:

subject

Has Abstract

pub_date

1992-10-01 00:00:00

pages

189-98

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

270

pub_type

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