Immunohistochemical localization of spermadhesin AWN in the porcine male genital tract.


:Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of spermatozoa was also uncertain. By using monospecific polyclonal antibodies against AWN, the immunohistochemical distribution pattern of AWN epitopes has been investigated in tissue sections of the porcine male genital tract. Our study has revealed that AWN is synthesized in the rete testis and in the epithelium of the seminal vesicles. The latter are also the major contributors of seminal plasma AWN. In addition, immunoblotting analysis has shown that AWN is present on epididymal spermatozoa. Our results indicate that the cellular origin of spermadhesins is species-specific. The attachment of AWN to epididymal spermatozoa is probably important in developing the capacity for fertilization.


Cell Tissue Res


Cell and tissue research


Sinowatz F,Amselgruber W,Töpfer-Petersen E,Calvete JJ,Sanz L,Plendl J




Has Abstract


1995-10-01 00:00:00












  • Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing.

    abstract::There is a current need for a therapy that can alleviate the social and economic burden that presents itself with debilitating and recurring musculoskeletal soft tissue injuries and disorders. Currently, several therapies are emerging and undergoing trials in animal models; these focus on the manipulation and administ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Gwyer D,Wragg NM,Wilson SL

    更新日期:2019-08-01 00:00:00

  • Gene expression of beta-catenin is up-regulated in inner dental epithelium and enamel knots during molar tooth morphogenesis in the mouse.

    abstract::Beta-catenin is a multi-functional molecule that is involved in both cell-cell adhesion and signaling. We analyzed changes in beta-catenin gene expression during mouse molar tooth development by in situ hybridization. Prominent up-regulation of the expression of this gene was evident exclusively in the enamel knot at ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Obara N,Suzuki Y,Takeda M

    更新日期:2006-07-01 00:00:00

  • FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle.

    abstract::Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Baptista IL,Silvestre JG,Silva WJ,Labeit S,Moriscot AS

    更新日期:2017-08-01 00:00:00

  • Depletion of macrophages and disappearance of postcapillary high endothelial venules in lymph nodes deprived of afferent lymphatic vessels.

    abstract::The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time period...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hendriks HR,Eestermans IL,Hoefsmit EC

    更新日期:1980-01-01 00:00:00

  • Signaling in the suprachiasmatic nucleus: selectively responsive and integrative.

    abstract::The suprachiasmatic nucleus (SCN) contains a biological clock that generates timing signals that drive daily rhythms in behaviors and homeostatic functions. In addition to this pacemaker function, the SCN gates its own sensitivity to incoming signals, which permits appropriate temporal adjustment to achieve synchrony ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Gillette MU,Mitchell JW

    更新日期:2002-07-01 00:00:00

  • Long live the liver: immunohistochemical and stereological study of hepatocytes, liver sinusoidal endothelial cells, Kupffer cells and hepatic stellate cells of male and female rats throughout ageing.

    abstract::Male/female differences in enzyme activity and gene expression in the liver are known to be attenuated with ageing. Nevertheless, the effect of ageing on liver structure and quantitative cell morphology remains unknown. Male and female Wistar rats aged 2, 6, 12 and 18 months were examined by means of stereological tec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Marcos R,Correia-Gomes C

    更新日期:2016-12-01 00:00:00

  • The distribution of endocrine cells in the mucosa of the gastrointestinal tract of the house musk shrew, Suncus murinus (Insectivora).

    abstract::The distribution of endocrine cells in the gastrointestinal tract of the house musk shrew, Suncus murinus (Family Soricidae, Order Insectivora) was studied immunohistochemically. The hormones investigated were gastrin, cholecystokinin (CCK), somatostatin, secretin, glucagon, gastric inhibitory polypeptide (GIP), motil...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kanamori Y,Nakazawa S,Kitoh J,Hoshino M

    更新日期:1989-11-01 00:00:00

  • Morphological heterogeneity of the smooth endoplasmic reticulum in the Leydig cells of the boar testis.

    abstract::The fine structure of boar Leydig cells is re-examined in samples post-fixed with OSO4-K4Fe(CN)6, an elective method to preserve the ultrastructural detail of the endocrine testis. The cells show a pleomorphic arrangement of the smooth endoplasmic reticulum. Large "vesicle-like" dilatations are interspersed in the tub...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Aguas AP

    更新日期:1983-01-01 00:00:00

  • Interdigitating reticulum cells in the popliteal lymph node of the rat. An ultrastructural and cytochemical study.

    abstract::Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is co...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Friess A

    更新日期:1976-07-20 00:00:00

  • Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera.

    abstract::We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Takeuchi H,Yasuda A,Yasuda-Kamatani Y,Sawata M,Matsuo Y,Kato A,Tsujimoto A,Nakajima T,Kubo T

    更新日期:2004-05-01 00:00:00

  • Glycoprotein secretion in the hypothalamo-neurohypophyseal system of the rat.

    abstract::Although the secretory products of the hypothalamo-neurohypophyseal system are not glycoproteins, synthesis and migration of these macromolecules occur within its secretory neurons. After being labeled with 3H-fucose in the Golgi apparatus, newly synthesized glycoproteins migrate to secretion granules, lysosomes and t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Haddad A,Guaraldo SP,Pelletier G,Brasileiro IL,Marchi F

    更新日期:1980-01-01 00:00:00

  • Immunocytochemical localization of beta-1,3-glucan recognition protein in the silkworm, Bombyx mori.

    abstract::A monospecific antibody against beta-1,3-glucan recognition protein (a 62 kDa protein) of the larval silkworm prophenoloxidase activating system was used to study the localization of the protein. Among tissues from 5th instar larvae, only hemocytes and plasma were shown to contain a 62 kDa polypeptide immunoreactive w...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ochiai M,Niki T,Ashida M

    更新日期:1992-06-01 00:00:00

  • Immune complexes of E. coli antigens and maternal IgG in the bursa of Fabricius.

    abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sonoda K,Noguchi K,Ekino S

    更新日期:2013-12-01 00:00:00

  • Immunocytochemical identification of enkephalinergic neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus.

    abstract::Our immunocytochemical investigation of the magnocellular neuroendocrine system in the goldfish hypothalamus reveals enkephalin (ENK)-containing neurons interspersed among the vasotocin (VT)- and isotocin (IT)-containing neurons of the preoptic nucleus. The perikarya of the ENK, VT, and IT neurons do not show distinct...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Reaves TA Jr,Hayward JN

    更新日期:1979-08-03 00:00:00

  • Irradiation influences the expression of substance P and enkephalin in the rat larynx.

    abstract::The effects of radiotherapy on neuropeptide expression in the rat larynx were studied. Irradiation was given for five days, 6 or 8 Gray daily. Ten days after the end of irradiation, the larynx, the laryngeal nerves and different ganglia related to the larynx were dissected out from irradiated and control animals and p...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lidegran M,Domeij S,Dahlqvist A,Henriksson R,Franzén L,Gustafsson H,Forsgren S

    更新日期:1995-01-01 00:00:00

  • The bridge-partitioning complex is absent in a distinct type of defective germ cell division in the gonads of the golden hamster.

    abstract::Intercellular bridges (IBs) connecting the cytoplasms of a defined type of defective germ cell division ("arrested mitoses") in male and female gonads of the immature golden hamster were studied by electron microscopy. In both sexes, such cells appear at the time when germ cells switch from mitotic proliferation to th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Miething A

    更新日期:2002-04-01 00:00:00

  • Shaping oral cell plasticity to osteogenic differentiation by human mesenchymal stem cell coculture.

    abstract::In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Proksch S,Steinberg T,Vach K,Hellwig E,Tomakidi P

    更新日期:2014-04-01 00:00:00

  • Quantitative analysis of rod-cored vesicles and dense granules of large granular lymphocytes in the liver, spleen, and peripheral blood of rats.

    abstract::Large granular lymphocytes (LGL) comprise a natural defense system in the liver and exert an inhibitory effect on tumor cell metastasis. In order to demonstrate the maturation of LGL in the liver from the morphological aspect, we evaluated electron-microscopically the frequency of 0.2 micron vesicles (rod-cored and "e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kaneda K,Pilaro AM,Sayers TJ,Nagashima K,Gonda MA,Ortaldo JR,Wiltrout RH

    更新日期:1994-04-01 00:00:00

  • Origin and development of unusual insect muscle tension receptors.

    abstract::This work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. The locust ovipositor muscle is the only insect system in which more than 100 tension receptor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Marinc C,Rose U

    更新日期:2007-12-01 00:00:00

  • Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina.

    abstract::During the first postnatal weeks of the developing rodent retina, rhodopsin can be detected in a number of neuron-like cells in the inner retina. In the present study, we aim to characterize the morphology, number and staining characteristics of this peculiar population. Misplaced rhodopsin-positive cells (MRCs) were ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Szabó K,Szabó A,Enzsöly A,Szél A,Lukáts A

    更新日期:2014-04-01 00:00:00

  • Cell differentiation during early development of Nassarius reticulatus L. (Gastropoda, Prosobranchia). II. Morphological changes of nuclei and nucleoi.

    abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schmekel L,Fioroni P

    更新日期:1976-05-13 00:00:00

  • Mitochondrial peptides-appropriate options for therapeutic exploitation.

    abstract::Besides their well-known function in cellular bioenergetics, the role of mitochondria in signaling regulation of cells homeostasis and survival has been uncovered during the past few decades. Possessing an independent genome and a unique genetic code, mitochondria biosynthesize protective stress response factors, the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Popov LD

    更新日期:2019-08-01 00:00:00

  • Distribution of laminin and types IV and III collagen in fetal, infant and adult human spleens.

    abstract::The immunohistochemical distribution of the basement membrane (BM) proteins, laminin and type IV collagen, and interstitial type III collagen was investigated in 12 fetal spleens at the 15th-38th gestational weeks (g.w.) and in spleens of 8 infants from term to 4 years. The results were compared with the distribution ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Liakka A,Apaja-Sarkkinen M,Karttunen T,Autio-Harmainen H

    更新日期:1991-02-01 00:00:00

  • Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress.

    abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bhatti FUR,Kim SJ,Yi AK,Hasty KA,Cho H

    更新日期:2018-10-01 00:00:00

  • Renewal and proliferation of spermatogonia during spermatogenesis in the Japanese quail, Coturnix coturnix japonica.

    abstract::Four different types of spermatogonia were identified in the seminiferous tubules of the Japanese quail: a dark type A (Ad), 2 pale A types (Ap1 and Ap2), and a type B. A model is proposed describing the process of spermatogonial development in the quail. The Ad spermatogonia are considered to be the stem cells. Each ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lin M,Jones RC

    更新日期:1992-03-01 00:00:00

  • Lysosomes and pancreatic islet function. A quantitative estimation of crinophagy in the mouse pancreatic B-cell.

    abstract::Ultrastructural studies of pancreatic islets have suggested that crinophagy provides a possible mechanism for intracellular degradation of insulin in the insulin-producing B-cells. In the present study, a quantitative estimation of crinophagy in mouse pancreatic islets was attempted by morphometric analysis of lysosom...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schnell AH,Swenne I,Borg LA

    更新日期:1988-04-01 00:00:00

  • Distribution of P75 neurotrophin receptor in adult human cochlea--an immunohistochemical study.

    abstract::Mechanisms underlying the unique survival property of human spiral neurons are yet to be explored. P75 (p75(NTR)) is a low affinity receptor for neurotrophins and is known to interact with Trk receptors to modulate ligand binding and signaling. Up-regulation of this receptor was found to be associated with apoptosis a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Liu W,Glueckert R,Kinnefors A,Schrott-Fischer A,Bitsche M,Rask-Andersen H

    更新日期:2012-06-01 00:00:00

  • Serum-free cryopreservation of porcine hepatocytes.

    abstract::The use of porcine hepatocytes in xenotransplantation, bioartificial liver support or pharmacological approaches demands serum-free cryopreservation protocols yielding high quality, viable, functional hepatocytes. Here, primary porcine hepatocytes were frozen without serum in liquid nitrogen by the use of a computer-a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Müller P,Aurich H,Wenkel R,Schäffner I,Wolff I,Walldorf J,Fleig WE,Christ B

    更新日期:2004-07-01 00:00:00

  • The ecdysis of hair mechanoreceptors in crayfish.

    abstract::The fine structure of hair mechanoreceptors in crayfish during moulting was investigated with special attention to the interface apparatus between cuticular hairs and sensory cells: the chorda. The chordae are lost with old exuviae at every moulting. They are drawn out from a moulting canal at the tip of the new hair....

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kouyama N,Shimozawa T

    更新日期:1984-01-01 00:00:00

  • Rat wct mutation induces a hypo-mineralization form of amelogenesis imperfecta and cyst formation in molar teeth.

    abstract::Our previous findings have demonstrated that the rat autosomal-recessive mutation, whitish chalk-like teeth (wct), induces enamel defects resembling those of human amelogenesis imperfecta (AI) in continuously growing incisor teeth. The present study clarifies the effect of the wct mutation on the morphogenesis and cal...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Osawa M,Kenmotsu S,Masuyama T,Taniguchi K,Uchida T,Saito C,Ohshima H

    更新日期:2007-10-01 00:00:00