Immunohistochemical localization of spermadhesin AWN in the porcine male genital tract.


:Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of spermatozoa was also uncertain. By using monospecific polyclonal antibodies against AWN, the immunohistochemical distribution pattern of AWN epitopes has been investigated in tissue sections of the porcine male genital tract. Our study has revealed that AWN is synthesized in the rete testis and in the epithelium of the seminal vesicles. The latter are also the major contributors of seminal plasma AWN. In addition, immunoblotting analysis has shown that AWN is present on epididymal spermatozoa. Our results indicate that the cellular origin of spermadhesins is species-specific. The attachment of AWN to epididymal spermatozoa is probably important in developing the capacity for fertilization.


Cell Tissue Res


Cell and tissue research


Sinowatz F,Amselgruber W,Töpfer-Petersen E,Calvete JJ,Sanz L,Plendl J




Has Abstract


1995-10-01 00:00:00












  • Biological and bioinformatical approaches to study crosstalk of long-non-coding RNAs and chromatin-modifying proteins.

    abstract::Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Backofen R,Vogel T

    更新日期:2014-06-01 00:00:00

  • Ovarian follicles of normal NMRI mice and homozygous "nude" mice. 1. Quantitative methodological study in the pubescent "nude" mouse.

    abstract::It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sprumont P

    更新日期:1976-07-30 00:00:00

  • Age-related decline of lymphoid-tissue components in the oral mucosa of the lip, cheek and soft palate of Macaca fascicularis.

    abstract::In three mature monkeys (Macaca fascicularis; 3.5 +/- 0.3 kg in weight), the labial, buccal and soft-palate mucosae were examined morphologically and stereologically. Using fixation by perfusion, standardized methods of tissue preparation and morphometric analysis at the light-microscopic level, the gross dimensions (...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schroeder HE,Dörig-Schwarzenbach A

    更新日期:1984-01-01 00:00:00

  • Categorization of the mitochondria-rich cells in the gill epithelium of the freshwater phases in the life cycle of lampreys

    abstract::The distribution and ultrastructure of the mitochondria-rich (MR) cells in the gills of larval (ammocoetes) and adult lampreys (Petromyzon marinus and Geotria australis) have been studied. One type of MR cell, which is found only in ammocoetes, occurs in groups on and between gill lamellae. Freeze-fracture replicas sh...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bartels H,Potter IC,Pirlich K,Mallatt J

    更新日期:1998-02-01 00:00:00

  • Precocious development of lectin (Ulex europaeus agglutinin I) receptors in dome epithelium of gut-associated lymphoid tissues.

    abstract::Dome epithelium (DE), the tissue covering lymphoid domes of gut-associated lymphoid tissues, was examined in both adult and neonatal rabbit appendix or sacculus rotundus to determine if dome epithelial cells matured earlier than epithelial cells covering adjacent villi. The localization of well-differentiated epitheli...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Roy MJ

    更新日期:1987-06-01 00:00:00

  • Studies on the fine structure of invertebrate blood vessels. II. The valves of the lateral sinus of the leech, Hirudo medicinalis L.

    abstract::The position, structure and function of the valves within the lateral sinus of the medical leech, Hirudo medicinalis, are described on the basis of vital, light- and electron microscopy. In this species the valvular apparatus consists of multiple elongated fir cone-shaped fibrous villi surrounding the orifices of the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hammersen F,Staudte HW,Möhring E

    更新日期:1976-09-20 00:00:00

  • Comparative ultrastructure of ureteric innervation.

    abstract::The distribution and structure of the ureteric nerves in a small series of mammals was compared with that previously demonstrated in the rat. There was marked interspecies variation in the extent to which the nerves penetrated the wall of the ureter and in the degree of development of the deep submucous plexus. In ani...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hoyes AD,Barber P,Martin BG

    更新日期:1975-07-23 00:00:00

  • Ultrastructure and distribution of somatostatin-like immunoreactive neurons and nerve fibres in the coeliac ganglion of cats.

    abstract::Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fehér E,Burnstock G

    更新日期:1991-03-01 00:00:00

  • Bone marrow-derived versus adipose-derived stem cells in wound healing: value and route of administration.

    abstract::The stem cells with their distinct ability of self-renewal and differentiation are considered an innovation in wound healing. However, there is lack of studies comparing the differential effect of the type and administration route of stem cells in the wound healing context. Thus, the current study has been designed to...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Aboulhoda BE,Abd El Fattah S

    更新日期:2018-11-01 00:00:00

  • Horseradish peroxidase in axons of the dorsal funiculi of the cat: distribution after an injection of the enzyme into the dorsal column nuclei.

    abstract::Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of R...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fabricius C,Berthold CH,Gatzinsky KP

    更新日期:1996-01-01 00:00:00

  • Location of PHM/VIP mRNA in human gastrointestinal tract detected by in situ hybridization.

    abstract::The expression of the gene for vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) in the human gastrointestinal tract was studied by in situ hybridization and Northern blotting for PHM/VIP mRNA and immunocytochemistry using specific antisera against the bioactive peptides PHM and VIP. In th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bredkjaer HE,Wulff BS,Emson PC,Fahrenkrug J

    更新日期:1994-05-01 00:00:00

  • Distribution of PACAP (pituitary adenylate cyclase-activating polypeptide)-like and helospectin-like peptides in the teleost gut.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) and helospectin are two vasoactive intestinal polypeptide (VIP)-related neuropeptides that have recently been demonstrated in the mammalian gut; the aim of this study was to reveal their occurrence and localisation in the gastrointestinal tract, swimbladder, u...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Olsson C,Holmgren S

    更新日期:1994-09-01 00:00:00

  • FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle.

    abstract::Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Baptista IL,Silvestre JG,Silva WJ,Labeit S,Moriscot AS

    更新日期:2017-08-01 00:00:00

  • Cell differentiation of the fetal rat anterior pituitary in vitro.

    abstract::Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Nemeskéry A,Németh A,Sétáló G,Vigh S,Halász B

    更新日期:1976-07-26 00:00:00

  • Fine structure of crystalline inclusions in B-cells of the islets of Langerhans in the alligator.

    abstract::The ultrastructure of crystalline beta granules of the islets of Langerhans in the alligator has been investigated. From optical diffraction analysis and serial sectioning, the existence of four distinct types of crystalline inclusions was established in ultrathin sections. The first type is the most frequent and is i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Raska I,Komrska J,Titlbach M,Rieder M

    更新日期:1978-03-13 00:00:00

  • Precocene-induced necrosis and haemocyte-mediated breakdown of corpora allata in nymps of the locust Locusta migratoria.

    abstract::Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schooneveld H

    更新日期:1979-11-01 00:00:00

  • Unique features of secretory granules observed in the pituitary growth hormone-secreting (GH) cells of the musk shrew (Suncus murinus L).

    abstract::Unique rod-shaped secretory granules were observed among oval or spherical secretory granules in GH cells of the anterior pituitary gland of musk shrew using the protein A-gold procedure combined with electron microscopy. The rod-shaped and spherical secretory granules were both immunoreactive by the immuno-gold metho...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ishibashi T,Shiino M

    更新日期:1988-01-01 00:00:00

  • Functional morphology of the primary olfactory centers in the brain of the hermit crab Coenobita clypeatus (Anomala, Coenobitidae).

    abstract::Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their periphera...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Polanska MA,Kirchhoff T,Dircksen H,Hansson BS,Harzsch S

    更新日期:2020-06-01 00:00:00

  • Electron-microscopic immunohistochemical study of the localization of immunoglobulin G in the choroid plexus of the rat.

    abstract::The localization of autologous antiperoxidase immunoglobulin G (IgG) was studied in the choroid plexus of Lewis rats immunized against horseradish peroxidase (HRP). This experiment was performed to study the permeability of the choroid plexus to intravascular IgG. It was shown that autologous IgG was present in the ex...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fleury J,Bellon B,Bernaudin JF,Bouchaud C,Pinchon MC,Kuhn J,Poirier J

    更新日期:1984-01-01 00:00:00

  • Hypertrophy of radial and ulnar arteries following denervation of the chicken wing.

    abstract::Denervation of radial and ulnar arteries in the growing and adult domestic fowl was achieved by unilateral sectioning of the brachial plexus. Eight weeks later the denervated arteries and those of the contralateral wing were examined with light- and electron microscopy to determine the effect of denervation on arteria...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hartley L,Campbell GR

    更新日期:1987-12-01 00:00:00

  • Expression of stem cell factors in the adult sea cucumber digestive tube.

    abstract::Homeostatic cell turnover has been extensively characterized in mammals. In their adult tissues, lost or aging differentiated cells are replenished by a self-renewing cohort of stem cells. The stem cells have been particularly well studied in the intestine and are clearly identified by the expression of marker genes i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Mashanov V,Zueva O,Mashanova D,García-Arrarás JE

    更新日期:2017-12-01 00:00:00

  • Thrombin regulates nerve growth factor secretion from vascular, but not bladder smooth muscle cells.

    abstract::The production of nerve growth factor (NGF) in peripheral organs may play a role in the pathophysiology of hypertension and in obstructive disorders of the bladder outlet. We have been examining the cellular processes of NGF delivery and secretion in smooth muscle. NGF secretion from vascular smooth muscle cells (VSMC...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sherer TB,Spitsbergen JM,Steers WD,Tuttle JB

    更新日期:1997-07-01 00:00:00

  • Cell type-specific localization of Ephs pairing with ephrin-B2 in the rat postnatal pituitary gland.

    abstract::Sox2-expressing stem/progenitor cells in the anterior lobe of the pituitary gland form two types of micro-environments (niches): the marginal cell layer and dense cell clusters in the parenchyma. In relation to the mechanism of regulation of niches, juxtacrine signaling via ephrin and its receptor Eph is known to play...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yoshida S,Kato T,Kanno N,Nishimura N,Nishihara H,Horiguchi K,Kato Y

    更新日期:2017-10-01 00:00:00

  • Iron loading in the livers of metamorphosing lampreys, Petromyzon marinus L.

    abstract::Iron loading of hepatocytes was followed through the stages (1-7) of metamorphosis in lamprey (Petromyzon marinus L.) using light- and electron-microscopic histochemistry. Iron is present in ferric and ferrous forms in the hepatocytes of larval lampreys in levels that can only be detected in the electron microscope. D...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Youson JH,Sargent PA,Sidon EW

    更新日期:1983-01-01 00:00:00

  • Immunocytochemical distribution of FMRFamide-like substance in the brain of the cloudy dogfish, Scyliorhinus torazame.

    abstract::The distribution of the molluscan cardioexcitatory tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2) in the brain of the cloudy dogfish, Scyliorhinus torazame, was examined by immunocytochemistry. FMRFamide-like immunoreactivity was demonstrated to occur extensively in various regions of the dogfish brain, except for the c...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Chiba A,Oka S,Honma Y

    更新日期:1991-08-01 00:00:00

  • Motor innervation by enteric nerve fibers containing both nitric oxide synthase and galanin immunoreactivities in the striated muscle of the rat esophagus.

    abstract::The relationship between nitric oxide synthase (NOS)- and galanin-immunoreactive nerve terminals and the origin of NOS-immunoreactive nerve terminals on the motor endplates in the striated muscles of the rat esophagus was investigated. Double immunohistochemical staining revealed a dual innervation of motor endplates ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kuramoto H,Kawano H,Sakamoto H,Furness JB

    更新日期:1999-02-01 00:00:00

  • Uptake and accumulation of tritiated uteroglobin by day-6 rabbit blastocysts.

    abstract::Uptake of uteroglobin (UGL) by day-6 rabbit blastocysts and the intracellular fate of this protein were studied by light- and electron-microscopic autoradiography, immunocytochemistry and acid-phosphatase cytochemistry. UGL, labelled with N-succinimidyl-(2-3-3H)-propionate, was administered to embryos in vitro for 25 ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dannhorn DR,Kirchner C

    更新日期:1990-12-01 00:00:00

  • Immunohistochemical evidence for the presence of tryptophan hydroxylase in the brains of insects as revealed by sheep anti-tryptophan hydroxylase polyclonal antibody.

    abstract::Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bao X,Tian X,Zhao Z,Qu Y,Wang B,Zhang J,Liu T,Yang L,Lv J,Song C

    更新日期:2008-06-01 00:00:00

  • Changes in retinal neuronal populations in the DBA/2J mouse.

    abstract::DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness cau...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Moon JI,Kim IB,Gwon JS,Park MH,Kang TH,Lim EJ,Choi KR,Chun MH

    更新日期:2005-04-01 00:00:00

  • Light- and scanning electron microscopical studies of the hypothalamo-adenohypophysial portal vessels of the toad Bufo bufo (L.).

    abstract::The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lametschwandtner A,Simonsberger P

    更新日期:1975-09-16 00:00:00