Abstract:
:The localization of (Na+-K+) ATPase in the intact electrocyte of the electric organ of Electrophorus electricus (L.) and its subcellular fractions was investigated by biochemical and cytochemical methods. The distribution of AChE activity in the subcellular fractions was also comparatively analysed with this enzyme serving as a marker of the innervated membranes of the electrocyte. After application of cytochemical method of Farquhar and Palade to glutaraldehyde-fixed tissue, reaction was observed only at the membranes of vesicles localized at the periphery of the electrocyte. Previously fixed electrocytes, incubated in Ernst's medium showed reaction only at the vesicles whereas in unfixed tissue reaction also appeared at other membranes (surface and invaginations) of the anterior and posterior faces. This reaction was significantly inhibited in the presence of ouabain or in the absence of K+. Inhibition of Na+-K+-ATPase by glutaraldehyde fixation was also confirmed by biochemical analysis.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Somló C,de Souza W,Machado RD,Hassón-Voloch Adoi
10.1007/BF00226673keywords:
subject
Has Abstractpub_date
1977-11-30 00:00:00pages
115-28issue
1eissn
0302-766Xissn
1432-0878journal_volume
185pub_type
杂志文章abstract::The fan-shaped body is the largest substructure of the central complex in Drosophila melanogaster. Two groups of large-field neurons that innervate the fan-shaped body, viz., F1 and F5 neurons, have recently been found to be involved in visual pattern memory for "contour orientation" and "elevation" in a rut-dependent...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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