Effects of cytochalasin B on pancreatic acinar cell structure and secretion.


:The effects of cytochalasin B (CB) on pancreatic structure and amylase release were studied by use of pancreatic fragments, isolated acini and isolated acinar cells. In pancreatic fragments and isolated acini CB caused the disappearance of microfilaments underlying the apical plasma membrane, loss of apical microvilli and luminal swelling, the last of which was greatly enhanced by addition of protein secretagogues. CB had no effect on basal amylase release but inhibited bethanechol-stimulated amylase in both fragments and acini. Isolated acinar cells, while retaining overall polarity, had lost most of the apical specialization including the microfilament and microvillous complex. Cells were still able to release amylase in response to bethanechol but this release was not affected by CB. The only structural effect of CB on isolated cells was margination of zymogen granules against the plasma membrane. This was, however, not accompanied by increased amylase release. It is concluded that microfilaments are important in maintaining the pancreatic acinar structure. Interference with this structure by CB leads to inhibition of bethanechol-stimulated amylase release. Microfilaments, however, may not play a direct role in secretion.


Cell Tissue Res


Cell and tissue research


Williams JA




Has Abstract


1977-04-29 00:00:00












  • Ultrastructural demonstration of nerve endings containing a substance related to growth hormone-releasing factor in the guinea-pig paraventricular nucleus.

    abstract::By means of a preembedding immuno-electron-microscopic technique, a large number of nerve endings containing a substance related to human growth hormone-releasing factor (hGRF) have been demonstrated in the paraventricular nucleus of the guinea pig. They made synaptic contacts primarily with dendritic shafts: 80% of t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Beauvillain JC,Tramu G,Mazzuca M

    更新日期:1987-04-01 00:00:00

  • Immunohistochemical study on the morphology of enterochromaffin cells in the human fundic mucosa.

    abstract::The morphology of enterochromaffin (EC) cells in the human fundic mucosa was investigated at the light-microscopic level by means of the unlabeled peroxidase anti-peroxidase method, with the use of a highly specific anti-serotonin serum. EC-cells in the human fundic mucosa were sparsely distributed below the neck port...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Inokuchi H,Kawai K,Takeuchi Y,Sano Y

    更新日期:1984-01-01 00:00:00

  • Presumptive insect circadian pacemakers in vitro: immunocytochemical characterization of cultured pigment-dispersing hormone-immunoreactive neurons of Leucophaea maderae.

    abstract::The accessory medulla with its associated pigment-dispersing hormone-immunoreactive neurons appears to be the pacemaker that controls the circadian locomotor activity rhythm of the cockroach Leucophaea maderae. To permit studies at the level of individual, identified, pacemaker neurons, we developed specific long-term...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Petri B,Stengl M

    更新日期:1999-06-01 00:00:00

  • Depletion of macrophages and disappearance of postcapillary high endothelial venules in lymph nodes deprived of afferent lymphatic vessels.

    abstract::The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time period...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hendriks HR,Eestermans IL,Hoefsmit EC

    更新日期:1980-01-01 00:00:00

  • Degranulation and appearance of vesicular inclusions in canine C cells after administration of antithyroid drug.

    abstract::When stimulated by the feeding of ethylenethiourea for a period of 6 months, dog thyroid glands increased to approximately 30 times their normal size. Not only follicular cells but also C cells had hyperplastic and hypertrophic features, compared with normal controls there was in the C cells a marked decrease in secre...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kameda Y

    更新日期:1982-01-01 00:00:00

  • Leucokinin and diuretic hormone immunoreactivity of neurons in the tobacco hornworm, Manduca sexta, and co-localization of this immunoreactivity in lateral neurosecretory cells of abdominal ganglia.

    abstract::Because leucokinins stimulate diuresis in some insects, we wished to identify the neurosecretory cells in Manduca sexta that might be a source of leucokinin-like neurohormones. Immunostaining was done at various stages of development, using an antiserum to leucokinin IV. Bilateral pairs of neurosecretory cells in abdo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Chen Y,Veenstra JA,Hagedorn H,Davis NT

    更新日期:1994-12-01 00:00:00

  • Atrial natriuretic peptide binding in rat placenta, yolk sac, decidua, and maternal placental vessels.

    abstract::Using in vitro autoradiography, binding sites of 125I-ANP (atrial natriuretic peptide) were localized in the rat placenta, visceral yolk sac, and decidua at 16, 18, and 20 days of gestation. There was diffuse binding over the labyrinthine region of the placenta and an intense binding over the decidual gland and viscer...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Scott JN

    更新日期:1993-05-01 00:00:00

  • Studies of isolated and enriched rat antral mucosa gastrin cells.

    abstract::A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to re...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Forssmann WG,Lichtenberger LM,Helmstaedter V,Ito S

    更新日期:1979-08-01 00:00:00

  • The visual system of the guitar fish (Rhinobatos productus).

    abstract::The retinal projections in adult and juvenile guitar fish (Rhinobatos productus) were determined with the aid of the Nauta-Fink-Heimer techniques. The visual system was found to be more extensive and more differentiated than in any other elasmobranch studied to date. Massive projections exist to the dorsal and ventral...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ebbesson SO,Meyer DL

    更新日期:1980-01-01 00:00:00

  • Actin filament organization of foot processes in vertebrate glomerular podocytes.

    abstract::We investigated the actin filament organization and immunolocalization of actin-binding proteins (alpha-actinin and cortactin) in the podocyte foot processes of eight vertebrate species (lamprey, carp, newt, frog, gecko, turtle, quail, and rat). Three types of actin cytoskeleton were found in these foot processes. (1)...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ichimura K,Kurihara H,Sakai T

    更新日期:2007-09-01 00:00:00

  • Retrograde labeling of regenerated electromotor neurons with HRP in a teleost fish, Sternarchus albifrons: relation to cell death.

    abstract::Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Anderson MJ,Fong HL,Waxman SG

    更新日期:1985-01-01 00:00:00

  • Regeneration of static-load-degenerated articular cartilage extracellular matrix by vitamin C supplementation.

    abstract::The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various ma...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sharma G,Saxena RK,Mishra P

    更新日期:2008-10-01 00:00:00

  • NBCe1 (SLC4A4) a potential pH regulator in enamel organ cells during enamel development in the mouse.

    abstract::During the formation of dental enamel, maturation-stage ameloblasts express ion-transporting transmembrane proteins. The SLC4 family of ion-transporters regulates intra- and extracellular pH in eukaryotic cells by cotransporting HCO3 (-) with Na(+). Mutation in SLC4A4 (coding for the sodium-bicarbonate cotransporter N...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Jalali R,Guo J,Zandieh-Doulabi B,Bervoets TJ,Paine ML,Boron WF,Parker MD,Bijvelds MJ,Medina JF,DenBesten PK,Bronckers AL

    更新日期:2014-11-01 00:00:00

  • Enkephalin-immunoreactive cells in the mesencephalic tegmentum project to the optic tectum of the teleosts Salmo gairdneri and Salmo salar.

    abstract::Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Vecino E,Ekström P,Sharma SC

    更新日期:1991-04-01 00:00:00

  • Fine structure of crystalline inclusions in B-cells of the islets of Langerhans in the alligator.

    abstract::The ultrastructure of crystalline beta granules of the islets of Langerhans in the alligator has been investigated. From optical diffraction analysis and serial sectioning, the existence of four distinct types of crystalline inclusions was established in ultrathin sections. The first type is the most frequent and is i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Raska I,Komrska J,Titlbach M,Rieder M

    更新日期:1978-03-13 00:00:00

  • Early effects of prolactin on lactating rabbit mammary gland. Ultrastructural changes and stimulation of casein secretion.

    abstract::Effects of prolactin on the secretion of milk proteins have been investigated by incubating mammary tissue fragments from lactating rabbits. Within 15 min of adding the hormone to the incubation medium, cell morphology is modified: the relative volume occupied by the Golgi region is greatly increased. When prolactin i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ollivier-Bousquet M

    更新日期:1978-02-14 00:00:00

  • Effects of dexamethasone on expression and maintenance of cartilage in serum-containing cultures of calvaria cells.

    abstract::The effects of dexamethasone on the ability of cells enzymatically isolated from 21-day fetal rat calvaria to produce cartilage in vitro has been investigated. Primary cultures of single-cell suspensions of rat calvaria were grown for up to 28 days in vitro in alpha-minimal essential medium containing 15% fetal bovine...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bellows CG,Heersche JN,Aubin JE

    更新日期:1989-04-01 00:00:00

  • Cerebrospinal fluid-contacting neurons in the paraventricular organ and in the spinal cord of the quail embryo: a fluorescence-histochemical study.

    abstract::Although the cerebrospinal fluid-contacting neurons of the avian paraventricular organ exhibit considerable amounts of catecholamines, they show no tyrosine hydroxylase immunoreactivity. In the quail embryo, the development of these neurons has been studied using the paraformaldeyde-glutaraldeyde method for the fluore...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Guglielmone R

    更新日期:1995-07-01 00:00:00

  • Development of striated rootlets during ciliogenesis in the human oviduct epithelium.

    abstract::Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the sam...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hagiwara H,Aoki T,Ohwada N,Fujimoto T

    更新日期:1997-10-01 00:00:00

  • Photoreceptor membrane breakdown in the spider Dinopis: localisation of acid phosphatases.

    abstract::The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blest AD,Price GD,Maples J

    更新日期:1979-07-17 00:00:00

  • Capsules of duck muscle spindles.

    abstract::Duck muscle spindles show a large outer capsule enclosing a number of intrafusal muscle fibres which are individually encircled by an inner capsule. The outer capsule consists of a multilayer of 4-6 flattened cells with elongated nuclei, and are usually lined by a basement membrane. They resemble and are linked with p...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Adal MN,Cheng SB

    更新日期:1980-01-01 00:00:00

  • The ultrastructure of an olfactory sensillum on the maxillary palps of locusta migratoria (L.).

    abstract::The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blaney WM

    更新日期:1977-11-07 00:00:00

  • The structure of symplasmic early oocytes and their enveloping sheath cells in the polychaete, Platynereis dumerilii.

    abstract::1. Early oocytes of Platynereis dumerilii are found in clusters floating in the coelom. The oocytes of a cluster form a syncytium which is enveloped by several sheath cells. 2. At stage 2, only a single sheath cell per cluster remains, and it penetrates the group of rounded oocytes, enveloping each one of them. At sta...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fischer A

    更新日期:1975-07-16 00:00:00

  • Expression of heat-shock protein 47 in mouse liver.

    abstract::Expression of heat-shock protein 47 in intact and fibrotic liver and in hepatic constituent cells was investigated in mice. Immunohistochemical study of intact liver and Western blot analysis of the protein from isolated liver cells revealed that stellate cells and smooth muscle cells of interlobular vessels, but not ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kawada N,Kuroki T,Kobayashi K,Inoue M,Nakatani K,Kaneda K,Nagata K

    更新日期:1996-05-01 00:00:00

  • Distribution of P75 neurotrophin receptor in adult human cochlea--an immunohistochemical study.

    abstract::Mechanisms underlying the unique survival property of human spiral neurons are yet to be explored. P75 (p75(NTR)) is a low affinity receptor for neurotrophins and is known to interact with Trk receptors to modulate ligand binding and signaling. Up-regulation of this receptor was found to be associated with apoptosis a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Liu W,Glueckert R,Kinnefors A,Schrott-Fischer A,Bitsche M,Rask-Andersen H

    更新日期:2012-06-01 00:00:00

  • Effects of bunaftine on morphology, microfilament integrity, and mitotic activity in cultured human fibroblasts and HeLa cells.

    abstract::Human fibroblasts and HeLa cells were treated with bunaftine (N-butyl-N-/2-(diethylamino)ethyl/-1-naphthalenecarboxamide ) in vitro. At concentrations of 0.5-2.0 mM, the drug caused contraction and rounding of the cells with loss of microvilli-like processes. Aggregates of dense, partly granular, partly fibrillar mate...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Moskalewski S,Thyberg J

    更新日期:1984-01-01 00:00:00

  • Central innervation of the pineal organ of the Mongolian gerbil. A histochemical and lesion study.

    abstract::Nerve fibers connecting the brain with the pineal gland of the Mongolian gerbil (central pinealopetal fibers) were investigated by means of light and electron microscopy. Several myelinated fibers penetrate from the brain into the deep pineal gland, extend further into the pineal stalk and continue to the superficial ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Møller M,Korf HW

    更新日期:1983-01-01 00:00:00

  • Elevated expression of iASPP correlates with poor prognosis and chemoresistance/radioresistance in FIGO Ib1-IIa squamous cell cervical cancer.

    abstract::iASPP (inhibitory member of the ASPP family), the conserved key inhibitor of p53, is overexpressed and plays an important oncogenetic role in many human cancers. However, its function in cervical cancer remains unknown. The objective of this study was to investigate the expression and clinical relevance of iASPP in ce...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Cao L,Huang Q,He J,Lu J,Xiong Y

    更新日期:2013-05-01 00:00:00

  • Ultrastructural localization of dipeptidyl peptidase IV in rat salivary glands by immunocytochemistry.

    abstract::The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical locali...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sahara N,Suzuki K

    更新日期:1984-01-01 00:00:00

  • Sympathectomy causes increased root resorption after orthodontic tooth movement in rats: immunohistochemical study.

    abstract::Accumulating evidence suggests that the sympathetic nervous system modulates inflammatory responses and bone remodeling. We have studied the effects of sympathectomy and orthodontic tooth movement (OTM) on root resorption, immunocompetent cell recruitment, neuropeptide, neurokinin-1 receptor (NK1-R), and interleukin 6...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Haug SR,Brudvik P,Fristad I,Heyeraas KJ

    更新日期:2003-08-01 00:00:00