Abstract：
:The effects of cytochalasin B (CB) on pancreatic structure and amylase release were studied by use of pancreatic fragments, isolated acini and isolated acinar cells. In pancreatic fragments and isolated acini CB caused the disappearance of microfilaments underlying the apical plasma membrane, loss of apical microvilli and luminal swelling, the last of which was greatly enhanced by addition of protein secretagogues. CB had no effect on basal amylase release but inhibited bethanechol-stimulated amylase in both fragments and acini. Isolated acinar cells, while retaining overall polarity, had lost most of the apical specialization including the microfilament and microvillous complex. Cells were still able to release amylase in response to bethanechol but this release was not affected by CB. The only structural effect of CB on isolated cells was margination of zymogen granules against the plasma membrane. This was, however, not accompanied by increased amylase release. It is concluded that microfilaments are important in maintaining the pancreatic acinar structure. Interference with this structure by CB leads to inhibition of bethanechol-stimulated amylase release. Microfilaments, however, may not play a direct role in secretion.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Williams JAdoi
10.1007/BF00219848subject
Has Abstractpub_date
1977-04-29 00:00:00pages
453-66issue
4eissn
0302-766Xissn
1432-0878journal_volume
179pub_type
杂志文章abstract：:The morphology of the antennal hair-sensilla of Periplaneta americana, their distribution and frequency on the antennal flagellum have been examined by transmission- and scanning-electron microscopy. The types of sensilla were distinguished with respect to physiologically relevant criteria such as wall structure and n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223221
更新日期：1978-07-13 00:00:00
abstract：:With an indirect immunofluorescence technique and an antiserum raised against synthetic LHRH, immunofluorescent granules were observed in fine radially oriented fibres in the palisade layer of the zoma externa in both the anterior and the posterior divisions of the median eminence in the greenfinch. There was no speci...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223264
更新日期：1975-09-16 00:00:00
abstract：:The diabetes (db/db) mutation (leptin-receptor defect) induces a hyperglycemic-hyperinsulinemic endometabolic environment that promotes hypercytolipidemic ovarian involution in C57BL/KsJ mice, resulting in reproductive sterility and eventual organoatrophy. The effectiveness of low-dose (1.0 microg/sc/3.5 day intervals...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0967-6
更新日期：2004-11-01 00:00:00
abstract：:Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318878
更新日期：1995-12-01 00:00:00
abstract：:The course of the Nervus connectivus (N.c.), its branches, and synaptic connections within the frontal ganglion (FG) were investigated electron microscopically after cobalt iontophoresis of the N.c. The subsequent treatment of ultrathin sections with Timm's method was found to be very suitable for identifying the smal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235172
更新日期：1979-01-01 00:00:00
abstract：:We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within the cells which renders them more detectable, rather than by a real increase in proliferation. The incorporation...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00338049
更新日期：1992-04-01 00:00:00
abstract：:The expression patterns of three microtubule-associated proteins (MAP1A, MAP1B, and MAP2A&B) were investigated in the developing optic tectum. Expression of MAP1B and middle-molecular-weight peptide of neurofilament (NF-M) was first observed in the same mesencephalic cells on day 3 of incubation, indicating that neuro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0796-z
更新日期：2003-12-01 00:00:00
abstract：:Intracellular sporozoan parasites invade the host cell through the invagination of the plasma membrane of the host and a vacuole is formed which accommodates the entering parasite. The vacuole may disappear and the invaginated membrane of the host then becomes closely apposed to that of the parasite's own membrane. As...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219605
更新日期：1976-06-28 00:00:00
abstract：:The peritoneal mesothelium of mouse embryos (12 to 18 day of gestation) was studied by freeze-fracture and in sections in order to reveal the initial formation of the tight junctions. Freeze-fracture observations showed three types of tight junctions. Type I consists of belt-like meshworks of elevations on the P face ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232008
更新日期：1979-05-18 00:00:00
abstract：:This study provides information on the rates of DNA synthesis, sites of DNA synthesis, and DNA content of the avian salt gland during the osmotic stressing (plasma membrane synthesis) and destressing (plasma membrane turnover) cycle, in an effort to better understand the relationship of cell turnover to the initial ev...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214782
更新日期：1982-01-01 00:00:00
abstract：:Retinal pigment epithelial cells from chicks at various stages of development were examined by transmission electron microscopy to determine how the adult form of the zonula adhaerens, composed of subunits termed zonula adhaerens complexes, is acquired. During early stages of development, between embryonic day 4 and e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01740771
更新日期：1990-03-01 00:00:00
abstract：:Neurointermediate lobes from adult or 10-day-old rats were implanted by a stereotaxic procedure into the third ventricle of adult male rats, in an area close to the paraventricular nucleus. They were examined, using immunocytochemical and ultrastructural techniques, at times ranging from 1 week to 8 months. All grafts...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224123
更新日期：1989-02-01 00:00:00
abstract：:In adrenalectomized rats the influence of salt loading or salt deprivation on the vasopressin and oxytocin content of the median eminence (ME) and the neural lobe (NL) was studied by means of various methods: morphometric and microphotometric analysis of aldehyde fuchsin-stained sections of ME and NL; immunohistochemi...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/BF00215904
更新日期：1986-01-01 00:00:00
abstract：:Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1149-x
更新日期：2005-09-01 00:00:00
abstract：:Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217887
更新日期：1983-01-01 00:00:00
abstract：:The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation of myenteric neurons and glial cells in paraffin-embedded colonic ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0770-5
更新日期：2009-05-01 00:00:00
abstract：:Type 1 diabetes is an autoimmune disorder characterized by a lack of insulin production by the beta cells of the pancreas. This lack of insulin causes a variety of systemic effects on whole-body metabolism. Poorly managed type 1 diabetes can lead to cardiovascular disease, diabetic neuropathy, and diabetic retinopathy...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1387-7
更新日期：2012-09-01 00:00:00
abstract：:The immunocytochemical localization of bovine oviduct-specific glycoproteins was investigated by light and electron microscopy. Using monoclonal antibodies (MAbs) specific for bovine oviductal glycoproteins, 3 regions (fimbriae, ampulla, and isthmus) of the epithelium in the bovine oviduct we studied during the follic...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327983
更新日期：1993-10-01 00:00:00
abstract：:Placenta-derived stem cells (PDSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal and differentiation and their immunomodulatory properties. Although many studies have characterized various PDSCs biologically, the properties of the sel...
journal_title：Cell and tissue research
pub_type： 临床试验,杂志文章
doi：10.1007/s00441-011-1249-8
更新日期：2011-10-01 00:00:00
abstract：:We demonstrate the existence of nerve fibers possessing substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactivity in the mouse cervical ventral roots. The distribution of the SP and CGRP fibers was similar, but CGRP fibers were generally more numerous. Both types entered the ventral pia mater or for...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327774
更新日期：1994-08-01 00:00:00
abstract：:The ciliary axoneme in photoreceptors from the retina of Xenopus laevis was examined by immunofluorescent staining of tubulin throughout the light/dark cycle. The immunofluorescent axoneme extended along only part of the length of rod outer segments but the entire length of cone outer segments. Both the cone axoneme a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050632
更新日期：1996-07-01 00:00:00
abstract：:The evolution of aquaporin-5 (AQP5) expression during postnatal development has not been defined in the sweat gland. Previous studies have suggested that AQP isoforms in several peripheral targets are regulated by a neural mechanism. We have examined, in rat sweat glands, the expression of AQP5 during postnatal develo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0399-1
更新日期：2007-07-01 00:00:00
abstract：:Ependymal cells located above the ventricular zone of the lateral, third, and fourth ventricles and the spinal cord are thought to form part of the adult neurogenic niche. Many studies have focused on ependymal cells as potential adult neural stem/progenitor cells. To investigate the functions of ependymal cells, a si...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03132-5
更新日期：2020-03-01 00:00:00
abstract：:With the use of immunocytochemistry, it was shown that both the supraoptic and paraventricular hypothalamic nuclei in humans contain at least two different neurophysins. These two human neurophysins are immunologically related to bovine neurophysin I and neurophysin II, respectively. One human neurophysin is associate...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00240096
更新日期：1979-02-15 00:00:00
abstract：:Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03044-4
更新日期：2019-11-01 00:00:00
abstract：:An attempt has been made to reveal which of the nine different types of synapses in the fundus striati, discriminated in a previous study, degenerate following experimental lesions in the parafasciculo-center median complex of the cat. Two types of synaptic contacts were found to be degenerated two days after the lesi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226045
更新日期：1976-09-06 00:00:00
abstract：:The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233613
更新日期：1980-01-01 00:00:00
abstract：:It is a well-established fact that the sympathetic, parasympathetic and enteric nervous systems are affected at early stages in Parkinson's disease (PD). However, it is not yet clarified whether the earliest pathological events preferentially occur in any of these three divisions of the autonomic nervous system (ANS)....
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-018-2851-9
更新日期：2018-07-01 00:00:00
abstract：:The thyroid cells of the cream hamster, characterized by abundance of microtubules and stratification of the organelles, undergo a particular evolution when the animals grow older. These changes are characterized by an increase of the number of lysosomes which in extreme cases become so prominent that they occupy the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215124
更新日期：1976-02-06 00:00:00
abstract：:R*-cells of the digestive gland of Carcinus maenas have been investigated functionally and morphologically. A comparison of the capacity of separated cell suspensions to synthesize glycogen gave support to the hypothesis that R and R* cells belong to the same cell line. The unexpected observation of R* cells in gastri...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318696
更新日期：1992-01-01 00:00:00
