Ultracytochemical evidence for a proton-pump adenosine triphosphatase in chick osteoclasts.

Abstract:

:The distribution of Mg++-ATPase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline pH cytochemical methods at the electron-microscopic level. Reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. Levamisole, ouabain, and vanadate did not affect the enzymatic activity. Para-chloromercuribenzoic acid (PCMB) prevented staining of mitochondria, ruffled border, and most cytoplasmic vesicles. Tri-n-butyltin decreased the amount of reaction product in cytoplasmic vesicles and ruffled-border membrane, but did not inhibit reaction product formation within mitochondria. Duramycin, which is a potent inhibitor for proton-pump ATPase, blocked reaction-product formation along the ruffled-border membrane, in mitochondria, and in cytoplasmic vesicles at alkaline pH, but not at neutral pH. It is concluded that the alkaline pH method for Mg++-ATPase appears to demonstrate sites of proton-pump ATPase activity.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Akisaka T,Gay CV

doi

10.1007/BF00218550

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

507-12

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

245

pub_type

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