Abstract:
:The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and electron-microscopic in situ hybridization, that H1t mRNA is detectable even in spermatogonia. Just a faint H1t band is seen in Western blots of nuclear protein from 9-day-old mice. This indicates that the H1t gene is expressed at premeiotic stages, albeit at a low level. In contrast to H1t mRNA, the H1t protein has not been detected in spermatogonia by electron microscopy after immunogold staining.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Drabent B,Bode C,Miosge N,Herken R,Doenecke Ddoi
10.1007/s004410050986subject
Has Abstractpub_date
1998-01-01 00:00:00pages
127-32issue
1eissn
0302-766Xissn
1432-0878journal_volume
291pub_type
杂志文章abstract::Chronic tinnitus (ringing of the ears) is a medically untreatable condition that reduces quality of life for millions of individuals worldwide. Most cases are associated with hearing loss that may be detected by the audiogram or by more sensitive measures. Converging evidence from animal models and studies of human ti...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-1992-8
更新日期:2015-07-01 00:00:00
abstract::SCO-spondin is a newly identified protein, strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When secreted into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, it condenses and forms Reissner's fiber. Several conserved domains have previously been charact...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051132
更新日期:1998-09-01 00:00:00
abstract::Structures identified as subsurface cisterns (SSC's) were found in neurons of the paraventricular nuclei of the rat hypothalamus. They appeared as cytoplasmic organelles consisting most often of stacks of parallel cisterns apposed to the neuronal plasmalemma. These SSC's were located in the interneurons of the parvoce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236138
更新日期:1979-06-27 00:00:00
abstract::Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of R...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050513
更新日期:1996-01-01 00:00:00
abstract::Growth/differentiation factor-15 (Gdf-15) is a member of the transforming growth factor-β (Tgf-β) superfamily and has been shown to be a potent neurotrophic factor for midbrain dopaminergic (DAergic) neurons both in vitro and in vivo. Gdf-15 has also been shown to be involved in inflammatory processes. The aim of this...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2406-x
更新日期:2016-08-01 00:00:00
abstract::The existence of the surface-connected canalicular system (SCCS) has been demonstrated in semithick sections of the frog thrombocytes by the use of a high voltage electron microscope. The SCCS of the thrombocytes in Rana catesbeiana and Rana nigromaculata consists of numerous canaliculi and vesicles with a diameter of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237001
更新日期:1979-10-01 00:00:00
abstract::Tanycytes in the median eminence (ME) of the rat exhibit morphological features suggesting their possible participation in transport phenomena. After adrenalectomy, which modifies the hypothalamo-hypophyseal axis, they undergo morphological changes characterized by an accumulation of lipid droplets, an increased numbe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215708
更新日期:1982-01-01 00:00:00
abstract::The spleen of the oriental fire-bellied toad, Bombina orientalis, consists of well-developed white pulp, separated from the lymphocytic marginal zone by the connective tissue boundary layer. Injection of peroxidase-conjugated rabbit anti-peroxidase revealed that these immune complexes were localized on the surface of ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00297553
更新日期:1993-01-01 00:00:00
abstract::Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug. In the distal elongating tips of myotubes, microtubules were the major cytoskeletal el...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214368
更新日期:1988-05-01 00:00:00
abstract::To obtain more information concerning the origin of dendritic reticulum cells, the development of germinal centers in the spleens of rabbits was investigated by conventional light microscopy, enzyme histochemistry, and electron microscopy. Washed sheep erythrocytes were used as antigen. Splenic tissue was examined on ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237632
更新日期:1980-01-01 00:00:00
abstract::Osteoarthritis (OA) is the most common joint disease with an unsatisfactory therapy outcome and characterized by the degradation of articular cartilage and synovial inflammation. Here, we isolated bone marrow mesenchymal stem cells (BMSCs) from rat's bone marrow and BMSC-derived exosome (BMSCs-Exo) from BMSCs successf...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03319-1
更新日期:2021-01-06 00:00:00
abstract::The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured fro...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00323584
更新日期:1993-04-01 00:00:00
abstract::Whether environmental exposure to bisphenol A (BPA) may induce reproductive disorders is still controversial but certain studies have reported that BPA may cause meiotic abnormalities in C. elegans and female mice. However, little is known about the effect of BPA on meiosis in adult males. To determine whether BPA exp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1723-6
更新日期:2014-01-01 00:00:00
abstract::The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP3R1) is predominantly expressed in cereb...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00307790
更新日期:1995-05-01 00:00:00
abstract::Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of sperma...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319144
更新日期:1995-10-01 00:00:00
abstract::The chemical composition of intermediate filaments (IF's) in the ependyma of the subcommissural organ (SCO) of the Mongolian gerbil (Meriones unguiculatus) was investigated immunohistochemically in paraffin-embedded tissue. Antibodies against glial fibrillary acidic protein (GFAP), vimentin, neurofilament proteins and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218796
更新日期:1989-03-01 00:00:00
abstract::M cells in Peyer's patch epithelium conduct transepithelial transport of luminal antigens to cells of the mucosal immune system. To determine the distribution of specific lectin-binding sites on luminal membranes of living M cells and to follow the transport route of membrane-bound molecules, lectin-ferritin conjugate...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215747
更新日期:1987-03-01 00:00:00
abstract::Neuromuscular terminals of a single motoneuron to four muscles (CPV7a, GM5a, CV2, and CV3) in the stomach of the blue crab Callinectes sapidus showed structural evidence for the exocytotic release of dense-core vesicles exclusively at synapses. The primary evidence was the appearance of dense cores in the synaptic cle...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050897
更新日期:1997-09-01 00:00:00
abstract::Since the advent of transmission electron microscopy of tissues capable of growth and regeneration, cell and developmental biologists have postulated that the undifferentiated cells observed within these tissues represent tissue-specific stem or progenitor cells. However, no studies have addressed the issue of whether...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0641-9
更新日期:2002-12-01 00:00:00
abstract::Iron loading of hepatocytes was followed through the stages (1-7) of metamorphosis in lamprey (Petromyzon marinus L.) using light- and electron-microscopic histochemistry. Iron is present in ferric and ferrous forms in the hepatocytes of larval lampreys in levels that can only be detected in the electron microscope. D...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217405
更新日期:1983-01-01 00:00:00
abstract::A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytoc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050884
更新日期:1997-08-01 00:00:00
abstract::We demonstrate the existence of nerve fibers possessing substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactivity in the mouse cervical ventral roots. The distribution of the SP and CGRP fibers was similar, but CGRP fibers were generally more numerous. Both types entered the ventral pia mater or for...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00327774
更新日期:1994-08-01 00:00:00
abstract::The apposition compound eyes of gonodactyloid stomatopods are divided into a ventral and a dorsal hemisphere by six equatorial rows of enlarged ommatidia, the mid-band (MB). Whereas the hemispheres are specialized for spatial vision, the MB consists of four dorsal rows of ommatidia specialized for colour vision and tw...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1118-4
更新日期:2005-08-01 00:00:00
abstract::Two populations of thymic lymphocytes from eight-week-old mice were separated based on the light scatter intensity they generated while passing through a flow cell analyser. The two populations were subsequently studied morphologically and analysed for the relative amount of DNA present in each cell. Cells having a lo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234855
更新日期:1979-09-01 00:00:00
abstract::Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2597-9
更新日期:2017-08-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. Since its discover...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03287-6
更新日期:2020-10-01 00:00:00
abstract::The tyrosine kinase proteins (Trk), encoded by the trk family of proto-oncogenes, mediate, in mammals, the action of neurotrophins, a family of growth factors acting on the development and maintenance of the nervous system. Neurotrophins and their specific receptors, TrkA, TrkB and TrkC, seem to be phylogenetically we...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000342
更新日期:2001-03-01 00:00:00
abstract::Ultrastructural aspects of hypertrophic chondrocytes in hamster and mouse epiphysial cartilage were examined in relation to their metabolic activities. With the hypertrophic change, cytoplasmic vacuolization proceeded leaving the partially intact endoplasmic reticulum (ER). In the hypertrophic cells, cytoplasmic hyalu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900082
更新日期:1999-11-01 00:00:00
abstract::Placing the clawed toad Xenopus laevis on a black background stimulates the melanotrope cells in the pars intermedia of the pituitary gland to release proopiomelanocortin (POMC)-derived peptides, including alpha-MSH and N-acetyl-beta-endorphin. In this study three types of secretory granules, electron-dense (approxima...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00314557
更新日期:1993-12-01 00:00:00
abstract::The spermatogonial stem cell line in prepubertal and adult bovine testis was studied by electron microscopy and protein gene product 9.5 immunohistochemistry. Three successive spermatogonia precursor cell configurations were observed. Small basal stem cells were found to possess a spherical shape and nuclei with two t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00583394
更新日期:1995-08-01 00:00:00