Abstract：
:Ultrastructural aspects of hypertrophic chondrocytes in hamster and mouse epiphysial cartilage were examined in relation to their metabolic activities. With the hypertrophic change, cytoplasmic vacuolization proceeded leaving the partially intact endoplasmic reticulum (ER). In the hypertrophic cells, cytoplasmic hyaluronan was stained with the biotinylated hyaluronan-binding region (b-HABR) of aggrecan, and mRNAs of hyaluronan synthase (Has 1, Has 2 and Has 3) were detected by in situ hybridization. When the epiphysial cartilage was cultured in the presence of 35S, 3H-GlcNAc, 3H-proline or 14C-palmitic acid, vacuolated late hypertrophic chondrocytes were labeled with these radioactive precursors. The evidence indicates that late hypertrophic chondrocytes are metabolically active, which appears to be essential for the enlargement of chondrocytes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Takada Y,Sakiyama H,Kuriiwa K,Masuda R,Inoue N,Nakagawa K,Itano N,Saito T,Yamada T,Kimata Kdoi
10.1007/s004419900082keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
317-25issue
2eissn
0302-766Xissn
1432-0878journal_volume
298pub_type
杂志文章abstract：:Rat ovarian perifollicular contractile tissue was examined at specified intervals prior to ovulation to determine the type, relative number, and length of cellular junctions. Rat ovaries were taken for electron-microscopic observation at 1500 h on the afternoon of proestrus (proestrus 0-h group), at 2000 h (proestrus ...
journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1987-07-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236132
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218550
更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233562
更新日期：1979-03-09 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233584
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224940
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1988-01-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1995-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1993-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214559
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236448
更新日期：1980-01-01 00:00:00
