Abstract：
:In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer's patches, and uptake by M cells was examined by electron microscopy. PLGA microspheres visualized as electron-lucent, spherical particles were taken up by M cells by pseudopod-like extensions of the M cell apical membrane and translocated to the pocket region containing mononuclear leukocytes within 60 min. These results indicate that PLGA microspheres can be directed to M cell apical surfaces for delivery to immunocompetent cells in gut-associated lymphoid tissues.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ermak TH,Dougherty EP,Bhagat HR,Kabok Z,Pappo Jdoi
10.1007/BF00318501subject
Has Abstractpub_date
1995-02-01 00:00:00pages
433-6issue
2eissn
0302-766Xissn
1432-0878journal_volume
279pub_type
杂志文章abstract：:Transforming growth factor-β (TGFβ) is the prototype for a large family of pleiotropic factors that signal via heterotetrameric complexes of type I and type II serine/threonine kinase receptors. Important intracellular mediators of TGFβ signaling are members of the Smad family. Smad2 and 3 are activated by C-terminal ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-011-1190-x
更新日期：2012-01-01 00:00:00
abstract：:We chose synaptic terminals of rat rod bipolar cells as a model system to study activity-related changes in the overall morphology and the fine structure of synaptic sites. Using confocal laser scanning microscopy in conjunction with three-dimensional reconstruction and electron microscopy, we examined the effect of l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051174
更新日期：1998-11-01 00:00:00
abstract：:Fueled by the discovery of head regeneration in triclads (planarians) two and a half centuries ago, flatworms have been the focus of regeneration research. But not all flatworms can regenerate equally well and to obtain a better picture of the characteristics and evolution of regeneration in flatworms other than plana...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03302-w
更新日期：2020-11-07 00:00:00
abstract：:Pituitary somatotrops were localized in four species from the genus Oncorhynchus using an antiserum to chum salmon (Oncorhynchus keta) growth hormone. The antiserum cross-reacted specifically with the acidophils in the caudal pars distalis of all species tested. Corroborative immunodiffusion studies were also conducte...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218126
更新日期：1983-01-01 00:00:00
abstract：:Ultrastructural post-embedding immuno-gold techniques were applied to the supraoptic nucleus and the neurohypophysis of mice and rats. The primary antibodies were three different monoclonal antineurophysins, used in protein A-gold and immunoglobulin-gold procedures. Conventional plastic embedding as well as hydrophili...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221868
更新日期：1986-01-01 00:00:00
abstract：:Three types of sensilla occurring on the lips and on the antennae of Peripatopsis moseleyi have been investigated by scanning and transmission electron microscopy. On the lips sensory spines can be found which contain numerous cilia originating from bipolar receptor cells. They reach the tip of the spine where the cut...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220613
更新日期：1977-02-14 00:00:00
abstract：:The location of the perikarya of LH-RH neurons in the rat hypothalamus and their pathways to the median eminence were studied by immunohistochemistry and radioimmunoassay after placing stereotaxic electrolytic lesions in several parts of the hypothalamus. The principal location of the cell somata was found to be in th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234184
更新日期：1979-05-25 00:00:00
abstract：:Single- and dual-labelling immunohistochemistry were used to determine the distribution and coexistence of neuropeptides in perivascular nerves of the large arteries and veins of the snake, Elaphe obsoleta, using antibodies for vasoactive intestinal polypeptide, substance P, calcitonin gene-related peptide, neuropepti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00353904
更新日期：1992-09-01 00:00:00
abstract：:Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0437-z
更新日期：2007-09-01 00:00:00
abstract：:The innervation pattern of distal muscle fibers of the opener muscle of walking legs of crayfish (Astacus leptodactylus) was investigated using methylene-blue staining, cobalt infiltration, and electron microscopy. A quantitative analysis of the entire innervation of single muscle fibers was attempted. It was found th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213750
更新日期：1982-01-01 00:00:00
abstract：:The three-dimensional structure of the mitochondria and sarcoplasmic reticulum (SR) in the three types of twitch fibers, i.e., the red, white and intermediate skeletal muscle fibers, of the vastus lateralis muscle of the Japanese meadow frog (Rana nigromaculata nigromaculata Hallowell) was examined by high resolution ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218939
更新日期：1987-12-01 00:00:00
abstract：:Changes in the organisation and composition of extracellular matrix in human endometrium during the menstrual cycle and early pregnancy have been assessed by immunofluorescence. Amongst interstitial components, type-III and type V-collagens and fibronectin are present in endometrial stroma throughout the menstrual cyc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221758
更新日期：1988-07-01 00:00:00
abstract：:Cells binding anti-bovine TASH beta serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundanc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234834
更新日期：1979-04-30 00:00:00
abstract：:Steroid hormones intervene in the structural and functional regulation of neuronal processes during development and thus determine brain differentiation. The effects of estrogens are mediated by two transcription factors, namely estrogen receptor α (ER-α) and estrogen receptor β (ER-β), that regulate the expression of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2023-5
更新日期：2015-02-01 00:00:00
abstract：:In fresh-water turtles, the bridge connecting the proximal and caudal stumps of transected spinal cords consists of regenerating axons running through a glial cellular matrix. To understand the process leading to the generation of the scaffold bridging the lesion, we analyzed the mitotic activity triggered by spinal i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1173-y
更新日期：2011-06-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:An immunohistochemical study for islet amyloid polypeptide (IAPP) was made on the gastrointestinal (GI) tract and pancreas of man and rat, using antisera raised against a synthetic peptide of C-terminal human IAPP (24-37) and a synthetic peptide of rat IAPP (18-37). A large number of IAPP-immunoreactive cells were fou...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305236
更新日期：1990-12-01 00:00:00
abstract：:The epithelial surface of the taste disc on the fungiform papillae of the frog was studied by means of freeze-fracture and conventional electron microscopy. The plasma membrane of the apical process of taste receptor cells was found to exhibit a uniform population of 10-nm intramembrane particles on its fracture face ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216883
更新日期：1982-01-01 00:00:00
abstract：:In the small intestine of the pig, neuromedin U (NMU)-immunoreactivity was mainly confined to the nerve plexus of the inner submucosal and mucosal regions. After colchicine treatment, a high number of immunoreactive nerve cell bodies was observed in the plexus submucosus internus (Meissner), whereas only a low number ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239453
更新日期：1989-11-01 00:00:00
abstract：:The formaldehyde-induced fluorescence technique had shown 5-hydroxytryptamine-containing enteric neurons in the intestine of the teleost Platycephalus bassensis, but did not reveal such neurons in the intestine of Tetractenos glaber or Anguilla australis. Re-examination of these animals with 5-hydroxytryptamine immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226505
更新日期：1988-01-01 00:00:00
abstract：:Besides their well-known function in cellular bioenergetics, the role of mitochondria in signaling regulation of cells homeostasis and survival has been uncovered during the past few decades. Possessing an independent genome and a unique genetic code, mitochondria biosynthesize protective stress response factors, the ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-019-03049-z
更新日期：2019-08-01 00:00:00
abstract：:The multicellular epithelial organs in Proteus anguinus, which Bugnion (1873) assumed to be developing neuromasts, have been analyzed by light- and electron-microscopy. Their fundamental structure consists of single ampullae with sensory and accessory cells with apical parts that extend into the pit of the ampulla, an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217865
更新日期：1984-01-01 00:00:00
abstract：:Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell population...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0282-5
更新日期：2007-01-01 00:00:00
abstract：:The effects of thyroidectomy, adrenalectomy, and castration on the pars distalis of male Japanese quail, and of injection of LH-RH on sexually inactive females, were investigated by light and electron microscopy. Correlation between light and electron microscopy was attained by use of alternate thin and thick sections...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219152
更新日期：1975-06-09 00:00:00
abstract：:Growth hormone, prolactin and somatostatin-like immunoreactivities were demonstrated in the brains of larval, young adult (parasitic) and upstream migrant adult sea lampreys, Petromyzon marinus, by means of immunoperoxidase techniques. Growth hormone (GH) and prolactin (PRL) were observed within separate perikarya in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218994
更新日期：1986-01-01 00:00:00
abstract：:Serial sections of human vaginal and keratinized oral-gingival epithelia were investigated for ciliary structures. Most melanocytes of the gingival epithelium lacked cilia, whereas almost all basal keratinocytes of the deeper portion of the epithelial ridges possessed one cilium each. In the suprabasal layers of the r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215830
更新日期：1988-02-01 00:00:00
abstract：:The neurosecretory cells of the ventral ganglia in the adult dragonfly, Orthetrum chrysis, are classified into A, B, C1 and C2 cells. The neurosecretory material in the ventral ganglia is composed of PAS-positive material with 1-, 2-glycol groups and some proteins. The proteins rich in cystine or cysteine occur predom...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221087
更新日期：1977-02-09 00:00:00
abstract：:Mice and rats emit and perceive calls in the ultrasonic range, i.e., above the human hearing threshold of about 20 kHz: so-called ultrasonic vocalizations (USV). Juvenile and adult rats emit 22-kHz USV in aversive situations, such as predator exposure and fighting or during drug withdrawal, whereas 50-kHz USV occur in...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1607-9
更新日期：2013-10-01 00:00:00
abstract：:An antiserum against conjugated histamine and two oligonucleotide probes that detect the mRNA encoding L-histidine decarboxylase (HDC) involved in histamine synthesis were used to study the appearance of histamine and its location in the kidneys of fetal, newborn and young postnatal rats and in the kidneys of pregnant...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302960
更新日期：1992-02-01 00:00:00
abstract：:Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217578
更新日期：1985-01-01 00:00:00
