Abstract:
:Hepatic stellate cells become activated and aquire contractility on being cultured. In order to characterize the morphology of contracted and relaxed stellate cells, we performed light- and electron-microscopic analyses of cultured stellate cells on collagen gels. Incubation of stellate cells with medium alone, 10 nM endothelin (ET)-1, or 1 mM N6,2' dibutyryladenosine 3':5'-cyclic monophosphate (dBcAMP) for 48 h induced contraction of the underlying collagen gels to 83%, 57%, and 97%, respectively, of their original size. Stellate cells relaxed by dBcAMP exhibited a round cell body and extended several long thin cytoplasmic processes with several varicosities. Culture with ET-1 accelerated spreading of the stellate cells on collagen gels and decreased the number of processes. Each such flattened stellate cell attached itself to the underlying collagen matrix by bending its cell body. Collagen fibers around the cell were pulled toward the cell and stretched. Thus, the present study has revealed that ET-1-stimulated cultured stellate cells adduct associated collagen fibers by the retraction of cytoplasmic processes and the bending of their spread cell bodies.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Kawada N,Harada K,Ikeda K,Kaneda Kdoi
10.1007/s004410050717subject
Has Abstractpub_date
1996-12-01 00:00:00pages
477-86issue
3eissn
0302-766Xissn
1432-0878journal_volume
286pub_type
杂志文章abstract::The peritoneal mesothelium of mouse embryos (12 to 18 day of gestation) was studied by freeze-fracture and in sections in order to reveal the initial formation of the tight junctions. Freeze-fracture observations showed three types of tight junctions. Type I consists of belt-like meshworks of elevations on the P face ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232008
更新日期:1979-05-18 00:00:00
abstract::The amniotic epithelium constitutes a diffusion barrier controlling the passage of solutes and water between the amniotic cavity and maternal circulation. With the present immunocytochemical approach, we have shown that several major components of the cyto-skeleton, i.e., actin, alpha-actinin, spectrin and ezrin, are ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318194
更新日期:1991-11-01 00:00:00
abstract::The distribution of serotonin-immunoreactive cells in the lung of 4 species of reptiles was investigated. Serotonin-containing cells were found forming groups in the interconnecting septa in all 4 species studied, and also as solitary cells in Testudo graeca and Mauremys caspica. Serotonin-containing cells were also l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216504
更新日期:1987-06-01 00:00:00
abstract::Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0596-6
更新日期:2008-06-01 00:00:00
abstract::The expression of corticosteroids and regional expression 16 (CR16) and neural Wiscott-Aldrich syndrome protein (N-WASP) was studied in the testes of men with idiopathic azoospermia by (1) immunohistochemistry and Western blot to detect the expression of CR16 and N-WASP protein, (2) reverse transcription with the poly...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-011-1159-9
更新日期:2011-06-01 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiot...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0636-2
更新日期:2008-08-01 00:00:00
abstract::The distribution of immunoreactive thyrotropin-releasing hormone (TRH) in the forebrain and hypophysis of Rana catesbeiana was studied by means of specific radioimmunoassay and immunohistochemistry based on peroxidase-antiperoxidase (PAP) techniques. A relatively high concentration of immunoassayable TRH is present in...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00212220
更新日期:1983-01-01 00:00:00
abstract::Golgi- and fluorescence-histochemical studies in the chicken shown the presence of a sharply delimited group of aminergic neurons beneath the floor of the fourth ventricle at the mesen-metencephalic boundary. According to the observations reported in other avian species a homology can be established between the mammal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216221
更新日期:1982-01-01 00:00:00
abstract::Neurointermediate lobes from adult or 10-day-old rats were implanted by a stereotaxic procedure into the third ventricle of adult male rats, in an area close to the paraventricular nucleus. They were examined, using immunocytochemical and ultrastructural techniques, at times ranging from 1 week to 8 months. All grafts...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224123
更新日期:1989-02-01 00:00:00
abstract::Peptidyl-glycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is an enzyme that catalyzes conversion of glycine-extended peptides to alpha-amidated bioactive peptides. Two peptides that are processed at their carboxyl-termini by this enzyme are neuropeptide Y and anglerfish peptide Y, both of which possess a C-te...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00304521
更新日期:1995-04-01 00:00:00
abstract::The existence of a neural crest cell migration pathway from occipital levels of the hindbrain into the heart was suspected in mammalian embryos because it had previously been identified in avian embryos and because the Di George anomaly, an association between craniofacial and cardiac malformations, is most easily exp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00338048
更新日期:1992-04-01 00:00:00
abstract::Cobaltous-lysine is transported anterogradely from the optic nerve of the teleost, Lethrinus chrysostomus (Lethrinidae, Perciformes). The marginal optic tract is labelled in longitudinal bands of light and dark staining fibres which persists caudally within the ventral division but not in the dorsal division. This spe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218889
更新日期:1989-01-01 00:00:00
abstract::The morphology of enterochromaffin (EC) cells in the human fundic mucosa was investigated at the light-microscopic level by means of the unlabeled peroxidase anti-peroxidase method, with the use of a highly specific anti-serotonin serum. EC-cells in the human fundic mucosa were sparsely distributed below the neck port...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226973
更新日期:1984-01-01 00:00:00
abstract::Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their periphera...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03199-5
更新日期:2020-06-01 00:00:00
abstract::A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03241-6
更新日期:2020-11-01 00:00:00
abstract::During tooth development, the inner and outer enamel epithelia fuse by mitotic activity to produce a bilayered epithelial sheath termed Hertwig's epithelial root sheath (HERS). The epithelial rests of Malassez (ERM) are the developmental residues of HERS and remain in the adult periodontal ligament (PDL). Although the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-1076-3
更新日期:2010-12-01 00:00:00
abstract::A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-1036-x
更新日期:2005-09-01 00:00:00
abstract::Unfertilized eggs commonly lack centrioles, which are usually provided by the male gamete at fertilization, and are unable to assemble functional reproducing centrosomes. However, some insect species lay eggs that develop to adulthood without a contribution from sperm. We report that the oocyte of the parthenogenetic ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0253-x
更新日期:2006-12-01 00:00:00
abstract::Although Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dent...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0655-z
更新日期:2008-09-01 00:00:00
abstract::An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218363
更新日期:1982-01-01 00:00:00
abstract::Urocortin (UCN) is a 40-amino-acid peptide and a member of the corticotropin-releasing hormone (CRH) family, which includes CRH, urotensin I, sauvagine, UCN2 and UCN3. The biological actions of CRH family peptides are mediated via two types of G-protein-coupled receptors, namely CRH type 1 receptor (CRHR1) and CRH typ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1450-4
更新日期:2012-10-01 00:00:00
abstract::Antibodies against cartilage proteins are highly prevalent in the sera and synovial fluids of rheumatoid arthritis (RA) patients and also precede disease induction in various spontaneous and induced animal models of arthritis. These antibodies play an important role in the induction and perpetuation of the clinical di...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-009-0816-8
更新日期:2010-01-01 00:00:00
abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1701-z
更新日期:2013-12-01 00:00:00
abstract::Prolonged secretory stimulation of the exocrine pancreas in the rat by in vivo infusion of caerulein leads to a rapid degranulation of the organ associated with a progressive reduction in the size of the zymogen granules. During the first six to twelve hours of stimulation Golgi complexes are enlarged and several stru...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224474
更新日期:1976-01-28 00:00:00
abstract::The mammalian airways are known to be richly innervated by several types of peptide-containing nerve fibers. Galanin-containing fibers are, however, comparatively few. The results of the present immunocytochemical study indicate that the chicken airways receive a notably dense supply of galanin-storing fibers. Other m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224722
更新日期:1989-04-01 00:00:00
abstract::The formation of secretory granules in chronically hypersecretory melanotrophs in the rat pituitary was studied. Hypersecretion was induced by treatment with the dopamine antagonist haloperidol (1.5 mg/kg daily for 7 days), which releases the normal neural dopaminergic inhibition of secretion from the melanotroph. Mor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319432
更新日期:1994-02-01 00:00:00
abstract::Cholinergic neurons have been revealed in the enteric nervous system by functional and biochemical studies but not by antibodies that provide excellent localisation of the synthesising enzyme, choline acetyltransferase (ChAT), in the central nervous system. In order to determine whether a newly described peripheral fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0652-6
更新日期:2003-01-01 00:00:00
abstract::Blue-green fluorescent subependymal cells with intraventricular processes were shown by the fluorescent histochemical method to be distributed from the preoptic recess to the infundibular recess of the frog hypothalamus. Electron microscopy revealed at least two types of CSF-contacting subependymal cells, type 1 conta...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221791
更新日期:1977-01-20 00:00:00
abstract::The immunoreactive CRF-neurons of the rat hypothalamus have been examined immunohistochemically employing anti-rat CRF serum. These neurons are confined to the paraventricular nucleus, dorsomedial-lateral hypothalamic area, and suprachiasmatic nucleus, and are, respectively, also immunoreactive to anti-Met-enk, -alpha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216347
更新日期:1985-01-01 00:00:00