Abstract:
:In marine cartilaginous fish, reabsorption of filtered urea by the kidney is essential for retaining a large amount of urea in their body. However, the mechanism for urea reabsorption is poorly understood due to the complexity of the kidney. To address this problem, we focused on elephant fish (Callorhinchus milii) for which a genome database is available, and conducted molecular mapping of membrane transporters along the different segments of the nephron. Basically, the nephron architecture of elephant fish was similar to that described for elasmobranch nephrons, but some unique features were observed. The late distal tubule (LDT), which corresponded to the fourth loop of the nephron, ran straight near the renal corpuscle, while it was convoluted around the tip of the loop. The ascending and descending limbs of the straight portion were closely apposed to each other and were arranged in a countercurrent fashion. The convoluted portion of LDT was tightly packed and enveloped by the larger convolution of the second loop that originated from the same renal corpuscle. In situ hybridization analysis demonstrated that co-localization of Na(+),K(+),2Cl(-) cotransporter 2 and Na(+)/K(+)-ATPase α1 subunit was observed in the early distal tubule and the posterior part of LDT, indicating the existence of two separate diluting segments. The diluting segments most likely facilitate NaCl absorption and thereby water reabsorption to elevate urea concentration in the filtrate, and subsequently contribute to efficient urea reabsorption in the final segment of the nephron, the collecting tubule, where urea transporter-1 was intensely localized.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Kakumura K,Takabe S,Takagi W,Hasegawa K,Konno N,Bell JD,Toop T,Donald JA,Kaneko T,Hyodo Sdoi
10.1007/s00441-015-2234-4subject
Has Abstractpub_date
2015-12-01 00:00:00pages
677-88issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-015-2234-4journal_volume
362pub_type
杂志文章abstract::Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051032
更新日期:1998-04-01 00:00:00
abstract::The dental follicle is an ectomesenchymal tissue that surrounds developing tooth germ and that contains osteoblastic-lineage-committed stem/progenitor cells. We examined the osteogenic potential of human dental follicle cells (hDFC) by microarray analysis. We first compared the characteristics of hDFC with those of hu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1477-6
更新日期:2012-11-01 00:00:00
abstract::Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224731
更新日期:1989-04-01 00:00:00
abstract::The pattern of innervation on individual iliotibialis muscle fibres from axolotl (Ambystoma mexicanum) has been investigated histologically and electrophysiologically. These polyneural fibres were found to be innervated on average at five end plate sites. The sites were distributed irregularly along each fibre. Averag...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219663
更新日期:1978-01-09 00:00:00
abstract::Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-1113-2
更新日期:2011-03-01 00:00:00
abstract::The fine structure of boar Leydig cells is re-examined in samples post-fixed with OSO4-K4Fe(CN)6, an elective method to preserve the ultrastructural detail of the endocrine testis. The cells show a pleomorphic arrangement of the smooth endoplasmic reticulum. Large "vesicle-like" dilatations are interspersed in the tub...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00211483
更新日期:1983-01-01 00:00:00
abstract::In humans, pathogenesis in cyclosporine A (CsA)-induced gingival overgrowth (GO) includes the accumulation of extracellular matrix (ECM) constituents, viz., collagen type-1 and type-3 and proteoglycans, in subgingival connective tissue. However, whether this increase is associated with alterations of molecules pivotal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0200-x
更新日期:2006-09-01 00:00:00
abstract::Ependymal cells located above the ventricular zone of the lateral, third, and fourth ventricles and the spinal cord are thought to form part of the adult neurogenic niche. Many studies have focused on ependymal cells as potential adult neural stem/progenitor cells. To investigate the functions of ependymal cells, a si...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-03132-5
更新日期:2020-03-01 00:00:00
abstract::The effects, on the maternal mammary gland, of diets containing similar lipid percentages but differing in composition of polyunsaturated fatty acids (PUFA) have been assessed in rats during pregnancy and lactation. For this purpose, tuna fish oil (an n-3-PUFA-enriched oil) and corn oil (an n-6-PUFA-enriched oil) were...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1523-4
更新日期:2013-03-01 00:00:00
abstract::We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0865-y
更新日期:2004-05-01 00:00:00
abstract::Cobaltous-lysine is transported anterogradely from the optic nerve of the teleost, Lethrinus chrysostomus (Lethrinidae, Perciformes). The marginal optic tract is labelled in longitudinal bands of light and dark staining fibres which persists caudally within the ventral division but not in the dorsal division. This spe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218889
更新日期:1989-01-01 00:00:00
abstract::We have recently examined the electrophysiology and ultrastructure of approximately 100 tactile spines from the metathoracic legs of adult cockroaches. In only one animal the single sensory neuron that innervates the spine was replaced with a pair of apparently identical neurons which we believe were both functional. ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216522
更新日期:1984-01-01 00:00:00
abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2857-3
更新日期:2018-10-01 00:00:00
abstract::Apoptotic and inflammatory processes occur in human arteriosclerotic lesions. We examined the hypothesis whether both processes are possibly associated by studying the colocalization of corresponding markers. In 11 human arteriosclerotic carotid arteries, proapoptotic markers (CPP32 (caspase-3), poly(ADP-ribose) polym...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0024-0
更新日期:2005-12-01 00:00:00
abstract::The fine structure and primary sensory projections of sensilla located in the labial-palp pit organ of the cotton bollworm Helicoverpa armigera (Insecta, Lepidoptera) are investigated by scanning electron and transmission electron microscopy combined with confocal laser scanning microscopy. The pit organ located on th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1657-z
更新日期:2013-09-01 00:00:00
abstract::Changes in the ultrastructure of the toad epidermis during the moulting cycle are described on the basis of 17 skin preparations fixed in consecutive phases of the cycle. Our previous light microscopical findings that morphological changes are mainly restricted to a short period prior to and after shedding are confirm...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221703
更新日期:1975-06-24 00:00:00
abstract::The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218204
更新日期:1987-05-01 00:00:00
abstract::The chemical nature of the thickened plasma membrane of cornified cells in stratified squamous epithelium was investigated in comparison with that in noncornified epithelium. Localizations of transglutaminase, molecular weight 92,000 daltons, and detection of epidermal cysteine proteinase inhibitor were effected with ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215516
更新日期:1987-08-01 00:00:00
abstract::Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultrastructural similar...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221380
更新日期:1976-10-06 00:00:00
abstract::The purpose of this study was to determine the cellular distribution and degradation in rat liver following intravenous injection of superparamagnetic iron oxide nanoparticles used for magnetic resonance imaging (NC100150 Injection). Relaxometric and spectrophotometric methods were used to determine the concentration ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0884-8
更新日期:2004-06-01 00:00:00
abstract::The membranes of the microvilli of UV- and green-photoreceptors of the ant Myrmecia gulosa have been studied with the freeze-fracture technique. Both inner fracture faces, the cytoplasmic P-face and the extracellular E-face, are covered by globular particles. The P-face particles appear to be randomly distributed, occ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218715
更新日期:1976-12-10 00:00:00
abstract::Electron-microscopic autoradiography of rat neurohypophyses treated with [3H] clonidine, an alpha 2-agonist, showed that binding apparently occurred preferentially at the neurosecretory endings and blood vessels rather than on the pituicytes. Since it is known that clonidine has a high affinity for plasma proteins, th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219223
更新日期:1986-01-01 00:00:00
abstract::Glutamate is the major excitatory neurotransmitter of the mammalian retina and glutamate uptake is essential for normal transmission at glutamatergic synapses. The reverse transcriptase-polymerase chain reaction (RT-PCR) has revealed the presence of three different high-affinity glutamate transporters in the rat retin...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050976
更新日期:1998-01-01 00:00:00
abstract::Periderm granules in the support cells of regenerating feathers of mature male Jungle Fowls were studied ultrastructurally and histochemically. Histochemical results showed the absence of carbohydrate and lipid, and the presence of protein in the periderm granules. The periderm granules were measured at successive lev...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220749
更新日期:1978-09-05 00:00:00
abstract::The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis vi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0744-z
更新日期:2009-03-01 00:00:00
abstract::The influence of adrenalectomy and administration of hypertonic saline on the amount of vasopressin, oxytocin, and neurophysin contained in the median eminence and the neural lobe of rats was studied by means of the following methods: (i) morphometric and microphotometric analyses of aldehyde fuchsin-stained histologi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00206271
更新日期:1983-01-01 00:00:00
abstract::The olfactory epithelium of mice after axotomy was investigated to clarify the stem cells of olfactory cells by double immunostaining using antikeratin (MA903) and anti-bromodeoxyuridine (BrdU) antibodies and by conventional electron microscopy. When a single dose of BrdU was given to mice 9 days after axotomy, immuno...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318179
更新日期:1991-11-01 00:00:00
abstract::Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050641
更新日期:1996-08-01 00:00:00
abstract::Fc and C3 receptors, which are characteristically present on macrophages, could not be demonstrated on osteoclasts maintained in situ on their normal substrates when assayed for by use of sheep red blood cells coated with immunoglobulin (Shapiro et al. 1979). The present study tested the hypotheses that Fc receptors a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236514
更新日期:1980-01-01 00:00:00
abstract::The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptid...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051042
更新日期:1998-04-01 00:00:00