Attenuated regenerative properties in human periodontal ligament-derived stem cells of older donor ages with shorter telomere length and lower SSEA4 expression.


:Periodontal ligament (PDL) stem cell properties are critical in the periodontal tissue regeneration for periodontitis. Previously, we have demonstrated that cigarette smoking attenuates PDL-derived stem cell (PDLSC) regenerative properties. Here, we report the findings on the regenerative properties of human PDLSCs with different donor ages and the underlying mechanisms. Human PDLSCs from 18 independent donors were divided into different age groups (≤ 20, 20-40, and > 40 years old). The proliferation of PDLSCs with donor age of ≤ 20 years old was significantly higher than that of the 20-40- and > 40-years-old groups, whereas the migration of PDLSCs with donor age of ≤ 20 and 20-40 years old was significantly higher than that of the > 40-years-old group. Moreover, the mesodermal lineage differentiation capabilities of PDLSCs were also higher in the donor age group of ≤ 20 years old than the donor age of > 40 years old. In addition, shorter telomere length and lower expression of SSEA4 were found in PDLSCs with donor age of > 40 years old, compared with those with donor age of ≤ 20-years-old group. Besides, PDLSCs with donor age of 20-40 and > 40 years old had higher IL6 and CXCL8 gene expressions. In summary, results from this study revealed the attenuated proliferation, migration, and mesodermal lineage differentiation properties in human PDLSCs with older donor ages. Donor age of PDLSCs should be considered as the selection criteria for the periodontal tissue regeneration treatment.


Cell Tissue Res


Cell and tissue research


Ng TK,Chen CB,Xu C,Xu Y,Yao X,Huang L,Liang JJ,Cheung HS,Pang CP,Huang Y




Has Abstract


2020-07-01 00:00:00














  • The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa.

    abstract::The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schürmann A,Axer H,Scheepers A,Doege H,Joost HG

    更新日期:2002-02-01 00:00:00

  • Sema3A chemorepellant regulates the timing and patterning of dental nerves during development of incisor tooth germ.

    abstract::Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Shrestha A,Moe K,Luukko K,Taniguchi M,Kettunen P

    更新日期:2014-07-01 00:00:00

  • Sequential expression of endothelial nitric oxide synthase, inducible nitric oxide synthase, and nitrotyrosine in odontoblasts and pulp cells during dentin repair after tooth preparation in rat molars.

    abstract::Nitric oxide (NO) stimulates osteoblast differentiation, but whether NO contributes to odontoblast differentiation during dentin repair is unknown. By using reverse transcription/polymerase chain reaction and immunostaining, we investigated the gene expression and/or immunolocalization of endothelial NO synthase (eNOS...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Mei YF,Yamaza T,Atsuta I,Danjo A,Yamashita Y,Kido MA,Goto M,Akamine A,Tanaka T

    更新日期:2007-04-01 00:00:00

  • The carbohydrates of secretory granules and the glycocalyx in developing mucoid cells.

    abstract::Complex carbohydrates in secretory granules and at the apical cell surface of mouse gastric mucoid cells were studied during embryogenesis and in the early postnatal period by various cytochemical methods; the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) and tannic acid-uranyl acetate (TA-UA) procedu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pipan N,Psenicnik M

    更新日期:1985-01-01 00:00:00

  • The ultrastructure of the protonephridial flame cell of the freshwater planarian Bdellocephala brunnea.

    abstract::The protonephridial flame cell of the freshwater planarian Bdellocephala brunnea was studied by electron microscopy. This basketlike cell with luminal cilia and fenestrae is known as a cyrtocyte. Careful examination revealed the actual continuity of the fenestrae over the surface of the cell and the microvillous natur...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ishii S

    更新日期:1980-01-01 00:00:00

  • Morphology and composition of the midgut bacterial community of Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae).

    abstract::The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Cossolin JFS,Lopes DRG,Martínez LC,Santos HCP,Fiaz M,Pereira MJB,Vivan LM,Mantovani HC,Serrão JE

    更新日期:2020-11-01 00:00:00

  • Synthesis of membrane and secretory glycoproteins by the acinar pancreatic cells as visualized by radioautography.

    abstract::Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labele...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Haddad A,Brasileiro IL

    更新日期:1983-01-01 00:00:00

  • Cell differentiation during early development of Nassarius reticulatus L. (Gastropoda, Prosobranchia). II. Morphological changes of nuclei and nucleoi.

    abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schmekel L,Fioroni P

    更新日期:1976-05-13 00:00:00

  • Synaptic input to the axon hillock and initial segment of inhibitory interneurons in the cerebellar cortex of the rat. An electron microscopic study.

    abstract::The axon hillock (AH) and initial segment (IS) of 10 Golgi neurons and 6 basket cells in the cerebellar cortex of the rat were investigated by electron microscopy using serial sections. An average of 10.4 and 11.3 synaptic terminals were observed to establish synaptic contact with the axon hillock region of Golgi and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hámori J

    更新日期:1981-01-01 00:00:00

  • A new type of gap junction in the phylum Brachiopoda.

    abstract::Gap junctions in the phylum Brachiopoda are shown to be of the P type as has been previously reported in invertebrates only in molluscs and possibly bryozoans. However, the mollusc gap junctions appear identical to vertebrae gap junctions in the fracture face on which particles remain, in particle spacing and in parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Flower NE,Green CR

    更新日期:1982-01-01 00:00:00

  • Regulation of secretory granule formation in chronically hypersecretory melanotrophs in the rat pituitary.

    abstract::The formation of secretory granules in chronically hypersecretory melanotrophs in the rat pituitary was studied. Hypersecretion was induced by treatment with the dopamine antagonist haloperidol (1.5 mg/kg daily for 7 days), which releases the normal neural dopaminergic inhibition of secretion from the melanotroph. Mor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bäck N,Soinila S

    更新日期:1994-02-01 00:00:00

  • The effect of sex hormones on the proximal tubules in the rat kidney.

    abstract::The three segments (S1, S2, S3) of the proximal tubule of the rat kidney were investigated, with special reference to lysosomes, after castration, estradiol application, and at the end of pregnancy. Especially in S1 and S2 castration induces an increase of cellular autophagy. The nuclei become smaller; endoplasmic ret...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schiebler TH,Danner KG

    更新日期:1978-09-26 00:00:00

  • Dislocated type-A spermatogonia in human seminiferous tubules.

    abstract::Type-A spermatogonia can be found in different locations throughout the germinal epithelium of human seminiferous tubules. Generally they represent the population of germ cells adjacent to the basal lamina, but under special conditions they may be located in the adluminal compartment. This aberrant location is found n...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Holstein AF,Bustos-Obregón E,Hartmann M

    更新日期:1984-01-01 00:00:00

  • Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT).

    abstract::This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Zhang J,Li F,Williamson KM,Tan S,Scott D,Onishi K,Hogan MV,Wang JH

    更新日期:2021-01-26 00:00:00

  • Candidate chemosensory cells in the stomach mucosa of young postnatal mice during the phases of dietary changes.

    abstract::The complex physiology of the gastrointestinal (GI) tract must permanently be adjusted according to the composition of ingested food, which requires continuous monitoring by appropriate sensory systems. Sensing the dietary constituents is thought to be mediated by chemosensory cells residing in the mucosa of the GI tr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sothilingam V,Hass N,Breer H

    更新日期:2011-05-01 00:00:00

  • High affinity choline transporter immunoreactivity in rat ileum myenteric nerves.

    abstract::Recently, an antibody against the choline transporter (CHT), an essential molecule involved in ACh uptake, was used to label cholinergic nerves in the central nervous system; however, the enteric nervous system (ENS) was not examined. The present study localised CHT immunoreactivity (CHT-IR) within the rat ileum ENS a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Harrington AM,Hutson JM,Southwell BR

    更新日期:2007-03-01 00:00:00

  • Immunocytochemical demonstration of met-enkephalin in the central nervous system of the domestic fowl.

    abstract::In the central nervous system of the male domestic flow, metenkephalin (ENK) immunoreactive perikarya and fiber tracts as well as extensive but sharply delimited fiber networks were visualized by means of the PAP technique. The most striking results were: (1) The demonstration of an association of ENK-containing struc...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blähser S,Dubois MP

    更新日期:1980-01-01 00:00:00

  • Ultrastructural changes in contents of membrane-coating granules after extrusion from epithelial cells of hamster cheek pouch.

    abstract::Membrane-coating granules of the epithelium of the hamster cheek pouch displayed a lamellated internal structure consisting of alternating thick and thin electron dense bands separated by translucent bands of equal width. The repeat distance of the contents was 8.5 nm +/- sd. 0.7 nm and the thickness of the major elec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hayward AF

    更新日期:1978-02-24 00:00:00

  • The midgut of Tomocerus minor Lubbock (insecta, collembola): ultrastructure, cytochemistry, ageing and renewal during a moulting cycle.

    abstract::The midgut cells of Tomocerus minor (Insecta, Collembola) were examined with the electron microscope and cytochemically. The midgut epithelium consists of a series of cells characterised by numerous mineral concretions scattered throughout the cytoplasm. Mitochondria are abundant; microvilli are well developed at the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Humbert W

    更新日期:1979-01-30 00:00:00

  • Quantitative histochemical studies of the effect of levamisole on splenic T-cells in thymectomized Bufo bufo larvae.

    abstract::The ability of levamisole (LVS) to correct the effect of thymectomy on splenic T-cell population in Bufo bufo larvae was tested. Using the nonspecific acid alpha-naphthyl acetate esterase (ANAE) technique, the T-lymphocytes displayed a characteristic reddish brown spot (T-pattern). The total number of white cells per ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Garavini C,Rossini MC,Mantovani B

    更新日期:1982-01-01 00:00:00

  • Metabolic preconditioning of mammalian cells: mimetic agents for hypoxia lack fidelity in promoting phosphorylation of pyruvate dehydrogenase.

    abstract::Induction of HIF-1α by oxygen limitation promotes increased phosphorylation and catalytic depression of mitochondrial pyruvate dehydrogenase (PDH) and an enhanced glycolytic poise in cells. Cobalt chloride and desferrioxamine are widely used as mimics for hypoxia because they increase the levels of HIF-1α. We evaluate...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Borcar A,Menze MA,Toner M,Hand SC

    更新日期:2013-01-01 00:00:00

  • The development and maintenance of smooth muscle in control and aneurogenic amphibians (Ambystoma).

    abstract::Ultrastructural investigations showed that development and maintenance of smooth muscle was similar in control and aneurogenic amphibian larvae. This applies to both multi-unit and unitary smooth muscles. The gut musculature displayed a regional variation in smooth muscle morphology and a variety of intermuscular appo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Tweedle CD

    更新日期:1976-02-12 00:00:00

  • The cercal receptor system of the praying mantid, Archimantis brunneriana Sauss. II. Cercal nerve structure and projection and electrophysiological responses of the individual receptors.

    abstract::The bilaterally paired cercal nerves of Archimantis brunneriana Sauss. leave the terminal ganglion posteriorly and then turn dorsally through muscles at the rear of the abdomen to enter the cerci, where each splits into two branches; successive branchings occur further distally in each cercus. In the distal nerve bran...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ball EE,Boyan GS,Stone RC

    更新日期:1982-01-01 00:00:00

  • Islet amyloid polypeptide (IAPP) in the gastrointestinal tract and pancreas of man and rat.

    abstract::An immunohistochemical study for islet amyloid polypeptide (IAPP) was made on the gastrointestinal (GI) tract and pancreas of man and rat, using antisera raised against a synthetic peptide of C-terminal human IAPP (24-37) and a synthetic peptide of rat IAPP (18-37). A large number of IAPP-immunoreactive cells were fou...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Toshimori H,Narita R,Nakazato M,Asai J,Mitsukawa T,Kangawa K,Matsuo H,Matsukura S

    更新日期:1990-12-01 00:00:00

  • Distribution of MAP1A, MAP1B, and MAP2A&B during layer formation in the optic tectum of developing chick embryos.

    abstract::The expression patterns of three microtubule-associated proteins (MAP1A, MAP1B, and MAP2A&B) were investigated in the developing optic tectum. Expression of MAP1B and middle-molecular-weight peptide of neurofilament (NF-M) was first observed in the same mesencephalic cells on day 3 of incubation, indicating that neuro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yasuda Y,Fujita S

    更新日期:2003-12-01 00:00:00

  • Interdigitating cells in the lymphoid tissues of bovine fetuses and calves. An electron-microscopic study.

    abstract::Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bielefeldt Ohmann H,Basse A

    更新日期:1984-01-01 00:00:00

  • Expression patterns of nm23 genes during mouse organogenesis.

    abstract::Nucleoside di-phosphate kinase enzyme (NDPK) isoforms, encoded by the nm23 family of genes, may be involved in various cellular differentiation and proliferation processes. We have therefore analyzed the expression of nm23-M1, -M2, -M3, and -M4 during embryonic mouse development. In situ hybridization data has reveale...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Amrein L,Barraud P,Daniel JY,Pérel Y,Landry M

    更新日期:2005-12-01 00:00:00

  • Autoradiographic localization of 3H-glucocorticoids and 3H-cortexolone in mouse pituitary.

    abstract::Autoradiograms of mouse pituitaries were prepared 30 min after injection of 3H-dexamethasone (3HDM), 3H-corticosterone (3HB) and 3H-cortexolone (3HS) either alone or in the presence of competing unlabelled steroids. 3H-dexamethasone accumulated in cell nuclei of both the pars distalis and the pars nervosa but not in t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Coutard M,Osborne-Pellegrin MJ,Funder J

    更新日期:1979-08-01 00:00:00

  • Regulation of glucose transport by thyroid hormone in rat ovary.

    abstract::Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ding Y,Tian Y,Guo M,Liu J,Heng D,Zhu B,Yang Y,Zhang C

    更新日期:2016-11-01 00:00:00

  • Precocene-induced necrosis and haemocyte-mediated breakdown of corpora allata in nymps of the locust Locusta migratoria.

    abstract::Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schooneveld H

    更新日期:1979-11-01 00:00:00