Attenuated regenerative properties in human periodontal ligament-derived stem cells of older donor ages with shorter telomere length and lower SSEA4 expression.


:Periodontal ligament (PDL) stem cell properties are critical in the periodontal tissue regeneration for periodontitis. Previously, we have demonstrated that cigarette smoking attenuates PDL-derived stem cell (PDLSC) regenerative properties. Here, we report the findings on the regenerative properties of human PDLSCs with different donor ages and the underlying mechanisms. Human PDLSCs from 18 independent donors were divided into different age groups (≤ 20, 20-40, and > 40 years old). The proliferation of PDLSCs with donor age of ≤ 20 years old was significantly higher than that of the 20-40- and > 40-years-old groups, whereas the migration of PDLSCs with donor age of ≤ 20 and 20-40 years old was significantly higher than that of the > 40-years-old group. Moreover, the mesodermal lineage differentiation capabilities of PDLSCs were also higher in the donor age group of ≤ 20 years old than the donor age of > 40 years old. In addition, shorter telomere length and lower expression of SSEA4 were found in PDLSCs with donor age of > 40 years old, compared with those with donor age of ≤ 20-years-old group. Besides, PDLSCs with donor age of 20-40 and > 40 years old had higher IL6 and CXCL8 gene expressions. In summary, results from this study revealed the attenuated proliferation, migration, and mesodermal lineage differentiation properties in human PDLSCs with older donor ages. Donor age of PDLSCs should be considered as the selection criteria for the periodontal tissue regeneration treatment.


Cell Tissue Res


Cell and tissue research


Ng TK,Chen CB,Xu C,Xu Y,Yao X,Huang L,Liang JJ,Cheung HS,Pang CP,Huang Y




Has Abstract


2020-07-01 00:00:00














  • Fibronectin and its relation to the basal lamina and to the cell surface in the chicken blastoderm.

    abstract::The ultrastructural distribution of fibronectin immunoreactivity was investigated in the chicken embryo during late gastrulation. Sites of binding of anti-fibronectin antibodies were ascribed to the basal lamina and associated structures, and to the cell surface. The fibronectin-rich basal lamina was resolved into a l...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Harrisson F,Vanroelen C,Vakaet L

    更新日期:1985-01-01 00:00:00

  • Enkephalin-immunoreactive cells in the mesencephalic tegmentum project to the optic tectum of the teleosts Salmo gairdneri and Salmo salar.

    abstract::Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Vecino E,Ekström P,Sharma SC

    更新日期:1991-04-01 00:00:00

  • Synaptic organization of the indoleamine-accumulating neurons in the cyprinid retina.

    abstract::The distribution and synaptic connections of the indoleamine-accumulating neurons in the retinae of the goldfish and carp were studied by means of fluorescence and electron microscopy. The indoleamine-accumulating neurons were visualized after intravitreal injection and uptake of the indoleamine 5,6-dihydroxytryptamin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Holmgren-Taylor I

    更新日期:1983-01-01 00:00:00

  • Chiton integument: ultrastructure of the sensory hairs of Mopalia muscosa (Mollusca: Polyplacophora).

    abstract::The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Leise EM,Cloney RA

    更新日期:1982-01-01 00:00:00

  • Evidence for neural progenitor cells in the human adult enteric nervous system.

    abstract::Putative neural stem cells have been identified within the enteric nervous system (ENS) of adult rodents and cultured from human myenteric plexus. We conducted studies to identify neural stem cells or progenitor cells within the submucosa of adult human ENS. Jejunum tissue was removed from adult human subjects undergo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Azan G,Low WC,Wendelschafer-Crabb G,Ikramuddin S,Kennedy WR

    更新日期:2011-05-01 00:00:00

  • Quantitative analysis of enteric neurons containing choline acetyltransferase and nitric oxide synthase immunoreactivities in the submucosal and myenteric plexuses of the porcine colon.

    abstract::The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals' intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric plexus (MP). This study quantifies enteric neurons in the ISP, OSP, and MP of the pig ascending (AC) and descending colon (DC) using the HuC/D,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Mazzoni M,Caremoli F,Cabanillas L,de Los Santos J,Million M,Larauche M,Clavenzani P,De Giorgio R,Sternini C

    更新日期:2020-09-23 00:00:00

  • The innervation pattern of crustacean skeletal muscle. Morphometry and ultrastructure of terminals and synapses.

    abstract::The innervation pattern of distal muscle fibers of the opener muscle of walking legs of crayfish (Astacus leptodactylus) was investigated using methylene-blue staining, cobalt infiltration, and electron microscopy. A quantitative analysis of the entire innervation of single muscle fibers was attempted. It was found th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Florey E,Cahill MA

    更新日期:1982-01-01 00:00:00

  • TrkA and TrkC neurotrophin receptor-like proteins in the lizard gut.

    abstract::The tyrosine kinase proteins (Trk), encoded by the trk family of proto-oncogenes, mediate, in mammals, the action of neurotrophins, a family of growth factors acting on the development and maintenance of the nervous system. Neurotrophins and their specific receptors, TrkA, TrkB and TrkC, seem to be phylogenetically we...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lucini C,de Girolamo P,Lamanna C,Botte V,Vega JA,Castaldo L

    更新日期:2001-03-01 00:00:00

  • Distribution of FMRFamide-like immunoreactivity in the brain, retina and nervus terminalis of the sockeye salmon parr, Oncorhynchus nerka.

    abstract::Neurons displaying FMRFamide(Phe - Met - Arg - Phe - NH2)-like immunoreactivity have recently been implicated in neural plasticity in salmon. We now extend these findings by describing the extent of the FMRF-like immunoreactive (FMRF-IR) system in the brain, retina and olfactory system of sockeye salmon parr using the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ostholm T,Ekström P,Ebbesson SO

    更新日期:1990-09-01 00:00:00

  • FGF2 and dexamethasone increase the production of hyaluronan in two-dimensional culture of elastic cartilage-derived cells: in vitro analyses and in vivo cartilage formation.

    abstract::Elastic cartilage-derived cells cultured two-dimensionally with FGF2 and corticosteroid produce gel-type masses that become mature cartilage when injected into a subcutaneous pocket. This unique method has previously been clinically applied for treatments of nasal augmentation. However, the components of the gel-type ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yokoyama A,Muneta T,Nimura A,Koga H,Mochizuki T,Hata Y,Sekiya I

    更新日期:2007-09-01 00:00:00

  • The major ganglion in the pelvic plexus of the male rat: a histochemical and ultrastructural study.

    abstract::To further evaluate the role of autonomic ganglia in the regulation of pelvic visceral activity, the neural elements in the major pelvic ganglion of the male rat have been studied with histochemixal and electron microscopic techniques. The principal findings are that the ganglion is composed of cholinergic and adrener...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dail WG,Evan AP,Eason HR

    更新日期:1975-05-27 00:00:00

  • Regeneration of static-load-degenerated articular cartilage extracellular matrix by vitamin C supplementation.

    abstract::The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various ma...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sharma G,Saxena RK,Mishra P

    更新日期:2008-10-01 00:00:00

  • Effects of dexamethasone on expression and maintenance of cartilage in serum-containing cultures of calvaria cells.

    abstract::The effects of dexamethasone on the ability of cells enzymatically isolated from 21-day fetal rat calvaria to produce cartilage in vitro has been investigated. Primary cultures of single-cell suspensions of rat calvaria were grown for up to 28 days in vitro in alpha-minimal essential medium containing 15% fetal bovine...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bellows CG,Heersche JN,Aubin JE

    更新日期:1989-04-01 00:00:00

  • Spermatogenesis in Anthozoa: differentiation of the spermatid.

    abstract::The fine structure of spermatids has been examined in Calliactis, Protanthea, Gonactinia and Parazoanthus (Cnidaria, Anthozoa). The sperm cells are relatively simple and lack distinct acrosomes. Their nuclei, spherical in the zoanthid, in the actinians are slendertipped cones. Condensation of the chromatin is interpre...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lyke EB,Robson EA

    更新日期:1975-01-01 00:00:00

  • Primary alveolar macrophages exposed to diesel particulate matter increase RAGE expression and activate RAGE signaling.

    abstract::Receptors for advanced glycation end-products (RAGE) are members of the immunoglobulin superfamily of cell-surface receptors implicated in mechanisms of pulmonary inflammation. In the current study, we test the hypothesis that RAGE mediates inflammation in primary alveolar macrophages (AMs) exposed to diesel particula...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Barton DB,Betteridge BC,Earley TD,Curtis CS,Robinson AB,Reynolds PR

    更新日期:2014-10-01 00:00:00

  • Structure of the auditory system of the weta Hemideina crassidens (blanchard, 1851) (Orthoptera, Ensifera, Gryllacridoidea, Stenopelmatidae). 1. Morphology and histology.

    abstract::The morphology and histology of the tibial auditory system of the New Zealand weta, Hemideina crassidens, are described. The groups of acoustic sensilla conform closely to the subgenual organ, intermediate organ and crista acoustica of the Tettigoniidae. Each prothoracic tibia bears two thick (40-100 micrometers) tymp...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ball EE,Field LH

    更新日期:1981-01-01 00:00:00

  • Morphological characterization of single fan-shaped body neurons in Drosophila melanogaster.

    abstract::The fan-shaped body is the largest substructure of the central complex in Drosophila melanogaster. Two groups of large-field neurons that innervate the fan-shaped body, viz., F1 and F5 neurons, have recently been found to be involved in visual pattern memory for "contour orientation" and "elevation" in a rut-dependent...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Li W,Pan Y,Wang Z,Gong H,Gong Z,Liu L

    更新日期:2009-06-01 00:00:00

  • Development and retention of phenotypically specialized cells in pituitary allografts in the hamster (Mesocricetus auratus).

    abstract::We used immunohistochemistry to identify cells present in pituitary allografts in the hamster. Hypophyses removed from neonatal hamsters or adenohypophyses removed from adult females were placed beneath renal capsules of hypophysectomized adult females. Serum PRL, LH, and GH concentrations were measured at two, five a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Campbell GT,Wagoner J,Colosi P,Soares MJ,Talamantes F

    更新日期:1988-01-01 00:00:00

  • Transient expression of the calcitonin receptor by enteric neurons of the embryonic and early post-natal mouse.

    abstract::Calcitonin receptor-immunoreactivity (CTR-ir) was found in enteric neurons of the mouse gastrointestinal tract from embryonic day 13.5 (E13.5) to post-natal day 28 (P28). CTR-ir occurred in cell bodies in ganglia of the myenteric plexus extending from the esophagus to the colon and in nerve cells of the submucosal gan...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Wookey PJ,Turner K,Furness JB

    更新日期:2012-02-01 00:00:00

  • Fine structure of the compound eyes and interfacetal mechanoreceptors of Cicindela tranquebarica Herbst (Coleoptera: Cicindelidae).

    abstract::The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kuster JE

    更新日期:1980-01-01 00:00:00

  • Neurotoxicant-induced animal models of Parkinson's disease: understanding the role of rotenone, maneb and paraquat in neurodegeneration.

    abstract::The etiologic basis of Parkinson's disease (PD), the second most common age-related neurodegenerative disorder, is unknown. Recent epidemiological and experimental studies indicate that exposure to environmental agents, including a number of agricultural chemicals, may contribute to the pathogenesis of this disorder. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Uversky VN

    更新日期:2004-10-01 00:00:00

  • Ultrastructural localization of radiolabelled L-dopa in the endocrine hypothalamus of the rat.

    abstract::Light and electron microscopic autoradiography has been employed to define the neuroanatomical patterns of uptake and binding of radiolabelled L-dopa in the endocrine hypothalamus of the rat. A dorsomedial continuum of arcuate and periventricular neurons selectively sequester 3HL-dopa 20 min following its intraventric...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Scott DE,Scott PM,Krobisch-Dudley G

    更新日期:1978-12-14 00:00:00

  • Acid phosphatase activity and intracellular collagen degradation by fibroblasts in vitro.

    abstract::Human gingival fibroblasts were cultured with collagen fibrils. The precise process of collagen phagocytosis and the relationship between acid phosphatase activity and intracellular degradation of collagen were investigated by cytochemical methods at the ultrastructural level. The collagen fibrils were first engulfed ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yajima T

    更新日期:1986-01-01 00:00:00

  • Lymph node macrophages and reticulum cells in the immune response. I. The primary response to paratyphoid vaccine.

    abstract::The reactivity of rat lymph node macrophages and reticulum cells has been studied in relation to the course of the immune response. Special attention was paid to the interdigitating cells (IDC) in the paracortex and the dendritic reticulum cells (DRC) in the germinal centre. Different types of macrophage predominate i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kamperdijk EW,Raaymakers EM,de Leeuw JH,Hoefsmit EC

    更新日期:1978-08-25 00:00:00

  • Candidate chemosensory cells in the stomach mucosa of young postnatal mice during the phases of dietary changes.

    abstract::The complex physiology of the gastrointestinal (GI) tract must permanently be adjusted according to the composition of ingested food, which requires continuous monitoring by appropriate sensory systems. Sensing the dietary constituents is thought to be mediated by chemosensory cells residing in the mucosa of the GI tr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sothilingam V,Hass N,Breer H

    更新日期:2011-05-01 00:00:00

  • Fine structure of the malaria parasite Plasmodium falciparum in human hepatocytes in vitro.

    abstract::Recent advances in the ability to culture the hepatic forms of mammalian malaria parasites, particularly of the important human pathogen Plasmodium falciparum have provided novel opportunities to study the ultrastructural organisation of the parasite in its natural host cell the human hepatocyte. In this electron-micr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Meis JF,Rijntjes PJ,Verhave JP,Ponnudurai T,Hollingdale MR,Smith JE,Sinden RE,Jap PH,Meuwissen JH,Yap SH

    更新日期:1986-01-01 00:00:00

  • Cutaneous glands of male and female impalas (Aepyceros melampus): seasonal activity changes and secretory mechanisms.

    abstract::The cutaneous glands of the forehead and the metatarsus were studied by histological and histochemical methods and electron microscopy in adult male and female impalas in various seasons of the year. All glandular areas consist of apocrine and holocrine glands, which, however, occur in different proportions. Our findi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Welsch U,van Dyk G,Moss D,Feuerhake F

    更新日期:1998-05-01 00:00:00

  • Scanning electron microscopic study of spermatozoa from gossypol-treated rats.

    abstract::Spermatozoa from the cauda epididymidis of gossypol-treated rats exhibit distinctive departures from the morphology of spermatozoa from control rats: wrinkled and disorganized cell membrane in the head and tail regions, cell membrane missing from segments of the tail midpiece and principal piece regions, malformed hea...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bozek SA,Jensen DR,Tone JN

    更新日期:1981-01-01 00:00:00

  • Cardiac dysfunction in transgenic mouse fetuses overexpressing shortened type XIII collagen.

    abstract::Overexpression of type XIII collagen molecules with an 83-amino-acid residue in-frame deletion of part of the ectodomain leads to fetal lethality in Col13a1COL2del transgenic mice. We characterize here the functional disturbances in the cardiovascular system of mouse fetuses overexpressing mutant type XIII collagen. D...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Tahkola J,Räsänen J,Sund M,Mäkikallio K,Autio-Harmainen H,Pihlajaniemi T

    更新日期:2008-07-01 00:00:00

  • Fine structure of the sensilla of Peripatopsis moseleyi (Onychophora).

    abstract::Three types of sensilla occurring on the lips and on the antennae of Peripatopsis moseleyi have been investigated by scanning and transmission electron microscopy. On the lips sensory spines can be found which contain numerous cilia originating from bipolar receptor cells. They reach the tip of the spine where the cut...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Storch V,Ruhberg H

    更新日期:1977-02-14 00:00:00