Abstract:
:An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect species that synthesize identical or structurally similar peptides. In the corpora cardiaca of species producing peptides with a different C-terminus, these cells remain unstained. Pea-CAH-I-like immunoreactivity has also been found in neurons of the central nervous system of all invertebrate orders studied. The antiserum recognizes the C-terminal sequence Pro-Asn-Trp-NH2 of the Pea-CAH-I molecule as established by enzyme immunoassay. The widespread Pea-CAH-I-like immunoreactivity in all nervous systems of the studied animals probably does not reflect the presence of Pea-CAH-I but the occurrence of peptides carrying similar epitopes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Eckert M,Gabriel J,Birkenbeil H,Greiner G,Rapus J,Gäde Gdoi
10.1007/s004410050601subject
Has Abstractpub_date
1996-06-01 00:00:00pages
401-13issue
3eissn
0302-766Xissn
1432-0878journal_volume
284pub_type
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