Abstract:
:Over the past decade, adult stem cells have attracted great attention because of their ability to potentially regenerate desired tissues or entire organs. With the emergence of nanomaterial-based gene therapy, adult stem cells have been considered as a proper tool for the biomedical field. In this study, we utilized organically modified silica (ORMOSIL) nanoparticles to deliver small interfering RNA (siRNA) against pigment epithelium-derived factor (PEDF) and induce the differentiation of human cardiac stem cells (CSCs). We found that the down-regulation of PEDF can inhibit the proliferation of human CSCs and induce cell differentiation. To further study the mechanism, we have tested the Notch signalling pathway genes, Hes1 and Hes5, and found that their expressions were inhibited by the PEDF down-regulation. Furthermore, with the restoration of PEDF, both the proliferation of human CSCs and expressions of Hes1 and Hes5 were recovered. Our results suggest for the first time the use of ORMOSIL as nanocarriers for the delivery of PEDF siRNA in human CSCs, and demonstrated the cooperation between PEDF and the Notch signalling pathway in maintaining the self-renewal and pluripotency of stem cells. PEDF as the essential controller in differentiation may be a promising target for the regulation of cardiac homeostasis and damage repair, which opens new treatment strategies using nanomaterials for heart disease therapy.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Liang H,Duan W,Hou H,Yi W,Zhang J,Jin Z,Zhai M,Yu S,Liu J,Yi Ddoi
10.1007/s00441-015-2235-3subject
Has Abstractpub_date
2015-12-01 00:00:00pages
611-21issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-015-2235-3journal_volume
362pub_type
杂志文章abstract::Electron-microscopic autoradiography of rat neurohypophyses treated with [3H] clonidine, an alpha 2-agonist, showed that binding apparently occurred preferentially at the neurosecretory endings and blood vessels rather than on the pituicytes. Since it is known that clonidine has a high affinity for plasma proteins, th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219223
更新日期:1986-01-01 00:00:00
abstract::Rats were injected with liposomes containing iodixanol (CTP10 Injection; 100 mg iodine per kg body weight) followed by a second injection of 125I-tyramine-cellobiose-albumin microspheres. The amounts of phagocytosed and degraded labelled albumin in liver were measured. A reduced uptake and degradation of albumin micro...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000229
更新日期:2000-06-01 00:00:00
abstract::Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219221
更新日期:1986-01-01 00:00:00
abstract::The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals' intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric plexus (MP). This study quantifies enteric neurons in the ISP, OSP, and MP of the pig ascending (AC) and descending colon (DC) using the HuC/D,...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03286-7
更新日期:2020-09-23 00:00:00
abstract::Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN + erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitocho...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214924
更新日期:1985-01-01 00:00:00
abstract::The smooth muscle of rabbit portal vein was studied by electron microscopy with particular emphasis on the mechanical linkage between the muscle cells and on the distribution of connective tissue. The media of this vein is composed of inner circular and outer longitudinal muscle layers which are orientated almost perp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237614
更新日期:1980-01-01 00:00:00
abstract::The three-dimensional structure of the mitochondria and sarcoplasmic reticulum (SR) in the three types of twitch fibers, i.e., the red, white and intermediate skeletal muscle fibers, of the vastus lateralis muscle of the Japanese meadow frog (Rana nigromaculata nigromaculata Hallowell) was examined by high resolution ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218939
更新日期:1987-12-01 00:00:00
abstract::The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233613
更新日期:1980-01-01 00:00:00
abstract::An attempt has been made to reveal which of the nine different types of synapses in the fundus striati, discriminated in a previous study, degenerate following experimental lesions in the parafasciculo-center median complex of the cat. Two types of synaptic contacts were found to be degenerated two days after the lesi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226045
更新日期:1976-09-06 00:00:00
abstract::Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyL...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051256
更新日期:1999-03-01 00:00:00
abstract::Considerable evidence indicates that the state of ocular connective tissues and their response in glaucomatous disease affect the degree of glaucoma damage. Both experimental and clinical data suggest that improved diagnostic and prognostic information can be derived from the assessment of the mechanical responsivenes...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1603-0
更新日期:2013-08-01 00:00:00
abstract::The comparatively simple Caenorhabditis elegans intestine fulfills many of the complex functions of the mammalian digestive tract, liver, and fat tissues, while also having roles in pathogen defense, immunity, and longevity. In this review, we describe the structure of the C. elegans gut and how it develops from the e...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03036-4
更新日期:2019-09-01 00:00:00
abstract::Islet amyloid peptide (or diabetes-associated peptide), the major component of pancreatic islet amyloid found in type-2 diabetes, has been identified by electron-microscopic immunocytochemistry in pancreatic B-cells from five non-diabetic human subjects, and in islets from five type-2 diabetic patients. The greatest d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221649
更新日期:1989-07-01 00:00:00
abstract::SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1618-6
更新日期:2013-06-01 00:00:00
abstract::The endothelial layer of blood vessels controls the passage of cells and solutes from the blood into the surrounding tissue. Crucial for this regulation is the integrity of endothelial cell-cell junctions. Various molecular mechanisms control junctional integrity of the endothelial layer including GTPases, modulation ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-1812-1
更新日期:2014-03-01 00:00:00
abstract::Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their periphera...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03199-5
更新日期:2020-06-01 00:00:00
abstract::Apart from the characteristic and progressive motor- and movement-related problems, Parkinson's disease (PD) patients also suffer from several non-motor symptoms, including gastrointestinal dysfunction. The fact that the enteric nervous system (ENS) controls motility and that one of the typical PD hallmarks, α-synucle...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-018-2856-4
更新日期:2018-07-01 00:00:00
abstract::The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons i...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1077-9
更新日期:2005-05-01 00:00:00
abstract::With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00-19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally, daily variations in protein content and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221865
更新日期:1986-01-01 00:00:00
abstract::The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233914
更新日期:1979-03-19 00:00:00
abstract::The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chrom...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218655
更新日期:1983-01-01 00:00:00
abstract::Electron-dense granules (EDGs) are singular structures found in the tissues of several vertebrate and invertebrate organisms. Two types of EDGs were observed in hepatopancreatic cells of the crab Ucides cordatus: (1) a non-mineralized EDG, found mainly inside vacuoles, which reacted positively to acid phosphatase and ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0646-4
更新日期:2003-01-01 00:00:00
abstract::A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234742
更新日期:1979-02-28 00:00:00
abstract::While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051092
更新日期:1998-06-01 00:00:00
abstract::Overexpression of type XIII collagen molecules with an 83-amino-acid residue in-frame deletion of part of the ectodomain leads to fetal lethality in Col13a1COL2del transgenic mice. We characterize here the functional disturbances in the cardiovascular system of mouse fetuses overexpressing mutant type XIII collagen. D...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0617-5
更新日期:2008-07-01 00:00:00
abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238295
更新日期:1983-01-01 00:00:00
abstract::A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218082
更新日期:1986-01-01 00:00:00
abstract::A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050800
更新日期:1997-04-01 00:00:00
abstract::Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0430-6
更新日期:2007-09-01 00:00:00
abstract::In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell-substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2122-y
更新日期:2015-08-01 00:00:00