Abstract：
:Microinjection of DNAase I, which is known to form a specific complex with G-actin, induces characteristic changes in cytoplasmic streaming, locomotion and morphology of the contractile apparatus of A. proteus. Light microscopical studies show pronounced streaming originating from the uroid and/or the retracting pseudopods, which ceases 10--15 min after injection of DNAase I, at a time when ultrasctructural studies show that the actin filament system is very much reduced. These results suggest that a controlled reversible equilibrium between soluble and polymerized forms of actin is a necessary requirement for amoeboid movement. The topographic distribution of contractile filaments beneath the plasma membrane visualized by correlated light- and electron microscopy of DNAase I-injected cells establishes the importance of the membrane-bound filamentous layer for three major aspects of streaming: (1) Streaming originates by local contractions of a cell membrane-associated filament layer at the uroid and/or retracting pseudopods, creating a pressure flow. (2) This flow continues beneath the membrane, which is stabilized by filaments in the lateral regions between the posterior end, with a high hydrostatic pressure, and the anterior end, with a low hydrostatic pressure. (3) Pseudopods or extending areas are created by a local destabilization of the cell periphery caused by the separation of the filamentous layer from the plasma membrane.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Wehland J,Weber K,Gawlitta W,Stockem Wdoi
10.1007/BF00236075subject
Has Abstractpub_date
1979-07-17 00:00:00pages
353-72issue
3eissn
0302-766Xissn
1432-0878journal_volume
199pub_type
杂志文章abstract：:From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
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journal_title：Cell and tissue research
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更新日期：1979-09-02 00:00:00
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pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-06-01 00:00:00
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更新日期：2002-10-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1976-07-20 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1990-09-01 00:00:00
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更新日期：1988-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1989-11-01 00:00:00
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更新日期：1978-06-08 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1981-01-01 00:00:00
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更新日期：1992-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2004-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1975-01-01 00:00:00
