The influence of high pressure freezing on mammalian nerve tissue.

Abstract:

:Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture repicas from rat cerebellar cortex and subfornical organ prepared by this method are compared to conventionally processed material using aldehyde fixation, glycerination and freezing with Freon. The formation of large ice crystals is prevented in tissue blocks up to 0.5 mm thick; deep etching is markedly enhanced. Cytoplasmic microstructures such as mitochondrial cristae, microtubules and microfilaments, are readily observable against a finely granulated cytosol matrix. An additional advantage is the combined application with freeze-substitution.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Moor H,Bellin G,Sandri C,Akert K

doi

10.1007/BF00237626

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

201-16

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

209

pub_type

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