Localization of juxtacrine factor ephrin-B2 in pituitary stem/progenitor cell niches throughout life.

Abstract:

:We have recently reported that Sox2-expressing pituitary stem/progenitor cells contact each other via a tight-junction protein CAR to form stem/progenitor cell niches in the marginal cell layer facing the lumen and in the clusters scattered in the parenchyma of the anterior lobe. However, the microenvironment of the niche for the maintenance of stem cell function in the pituitary remains obscure. In this study of pituitary stem/progenitor cell niches, we have attempted to identify the expression of juxtacrine factor ephrin and its receptor. We have found that ephrin-B2 is expressed in the pituitary throughout development but changes its localization pattern. Notably, in the adult pituitary, ephrin-B2 immuno-signals occur in SOX2-, E-cadherin-, and CAR-triple-positive stem/progenitor cells in the niches. Our data suggest that ephrin-B2 signaling has an important role in the formation of pituitary stem/progenitor cell niches and in pituitary organogenesis.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Yoshida S,Kato T,Higuchi M,Chen M,Ueharu H,Nishimura N,Kato Y

doi

10.1007/s00441-014-2054-y

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

755-66

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

359

pub_type

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