Enrichment of fibrillar cytoplasmic actomyosin in protoplasmic strands of Physarum polycephalum for the production of cell-free models.

Abstract:

:The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. The quantity of fibrillogenesis as well as the concomitant force output during the induced contractures are dependent on the Ca++-content and the temperature of the test solution. The method was developed to optimize the structure of the plasmodial strands before their subsequent transformation into cell-free models by permeabilization and extraction of the strands. Cryosections of plasmodial strands containing cytoplasmic actomyosin fibrils stained with fluorescently labeled phallotoxins offer a further assay for the study of their contraction physiology under cell-free conditions.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Pies NJ,Wohlfarth-Bottermann KE

doi

10.1007/BF00218017

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

365-74

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

239

pub_type

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