Abstract:
:The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. The quantity of fibrillogenesis as well as the concomitant force output during the induced contractures are dependent on the Ca++-content and the temperature of the test solution. The method was developed to optimize the structure of the plasmodial strands before their subsequent transformation into cell-free models by permeabilization and extraction of the strands. Cryosections of plasmodial strands containing cytoplasmic actomyosin fibrils stained with fluorescently labeled phallotoxins offer a further assay for the study of their contraction physiology under cell-free conditions.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Pies NJ,Wohlfarth-Bottermann KEdoi
10.1007/BF00218017subject
Has Abstractpub_date
1985-01-01 00:00:00pages
365-74issue
2eissn
0302-766Xissn
1432-0878journal_volume
239pub_type
杂志文章abstract::Nucleoside di-phosphate kinase enzyme (NDPK) isoforms, encoded by the nm23 family of genes, may be involved in various cellular differentiation and proliferation processes. We have therefore analyzed the expression of nm23-M1, -M2, -M3, and -M4 during embryonic mouse development. In situ hybridization data has reveale...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0036-9
更新日期:2005-12-01 00:00:00
abstract::The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237730
更新日期:1979-06-08 00:00:00
abstract::The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223268
更新日期:1975-09-16 00:00:00
abstract::The formaldehyde-induced fluorescence technique had shown 5-hydroxytryptamine-containing enteric neurons in the intestine of the teleost Platycephalus bassensis, but did not reveal such neurons in the intestine of Tetractenos glaber or Anguilla australis. Re-examination of these animals with 5-hydroxytryptamine immuno...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226505
更新日期:1988-01-01 00:00:00
abstract::Male/female differences in enzyme activity and gene expression in the liver are known to be attenuated with ageing. Nevertheless, the effect of ageing on liver structure and quantitative cell morphology remains unknown. Male and female Wistar rats aged 2, 6, 12 and 18 months were examined by means of stereological tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2490-y
更新日期:2016-12-01 00:00:00
abstract::The ontogeny of two gonadotropin-releasing-hormone (GnRH) systems, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), was investigated in zebrafish (Danio rerio). In situ hybridization (ISH) first detected sGnRH mRNA-expressing cells at 1 day post-fertilization (pf) anterior to the developing olfactory organs. Subseq...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0279-0
更新日期:2007-02-01 00:00:00
abstract::The cellular response of non-neuronal elements of the pyramidal cell layer of the rat hippocampus, especially the area CA3, was observed electron microscopically following destruction of this formation by means of intraventricular administration of kainic acid (KA). The neuroglial cell types responding to the KA-induc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238652
更新日期:1981-01-01 00:00:00
abstract::Freeze-fracture replicas of the rat corpus callosum revealed prominent junctional strands in fractured cell membranes of the somata of oligodendrocytes. The junctional strands were characterized by an elaborate system of straight or slightly undulating rows of linear aggregates of particles or ridges in the P face and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220534
更新日期:1977-10-21 00:00:00
abstract::Golgi- and fluorescence-histochemical studies in the chicken shown the presence of a sharply delimited group of aminergic neurons beneath the floor of the fourth ventricle at the mesen-metencephalic boundary. According to the observations reported in other avian species a homology can be established between the mammal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216221
更新日期:1982-01-01 00:00:00
abstract::It is hard to fathom that one of the most highly differentiated cells in the body, the spermatozoon, spends over half of its developmental life without the capacity for nuclear protein biosynthesis. This is even more incredible when considering that protein synthesis is switched off long before the sperm is mature. As...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-015-2249-x
更新日期:2016-01-01 00:00:00
abstract::The thoracic legs of the moth Manduca sexta acquire a new form and develop a new complement of sensory organs and muscles during metamorphosis from larva to adult. Because of our interest in the reorganization of neural circuitry and the acquisition of new behaviors during metamorphosis, we are characterizing sensory ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318442
更新日期:1990-02-01 00:00:00
abstract::Studies with environmental levels of various metals typically focus on observable neurological symptoms in newborns and adults. Use of the C2C12 skeletal muscle cell line as a developmental model enabled us to test whether environmental insults prevented myotube formation or the assembly of the postsynaptic component ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0878-6
更新日期:2004-05-01 00:00:00
abstract::Andersen's syndrome (AS) is a rare autosomal disorder that has been defined by the triad of periodic paralysis, cardiac arrhythmia, and developmental anomalies. AS has been directly linked to over 40 different autosomal dominant negative loss-of-function mutations in the KCNJ2 gene, encoding for the tetrameric strong ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2696-7
更新日期:2018-02-01 00:00:00
abstract::Brain injuries trigger physiological reactions which are mediated by a number of cytokines including interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and leukemia inhibitory factor (LIF). Astrocytes and microglia, the protagonists in these traumatic responses, are known to secrete a variety of paracrine signa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0624-x
更新日期:2002-11-01 00:00:00
abstract::Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2453-3
更新日期:2016-11-01 00:00:00
abstract::The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were compa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209040
更新日期:1978-10-17 00:00:00
abstract::The prothoracicotropic hormone (PTTH), which stimulates ecdysteroid synthesis in the prothoracic glands, is produced, in the dorso-lateral protocerebrum of Manduca sexta, by paired peptidergic neurons, the lateral neurosecretory cell group III (L-NSC III). Our study revealed ultrastructural features of L-NSC III, iden...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00303082
更新日期:1994-07-01 00:00:00
abstract::The ultrastructural distribution of fibronectin immunoreactivity was investigated in the chicken embryo during late gastrulation. Sites of binding of anti-fibronectin antibodies were ascribed to the basal lamina and associated structures, and to the cell surface. The fibronectin-rich basal lamina was resolved into a l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217185
更新日期:1985-01-01 00:00:00
abstract::Vasodilator-stimulated phosphoprotein (VASP) and mammalian-enabled protein (MENA) share similar cellular localisation and functions (signal transduction pathways, regulation of actin cytoskeleton dynamics). Functional substitution and compensation among Ena/VASP proteins have been proposed as the reason for the absenc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0964-9
更新日期:2004-12-01 00:00:00
abstract::Identified dorsal unpaired median (DUM) neurones of the locust Locusta migratoria were stained intracellularly with large amounts of cobalt to reveal their extensive peripheral branching patterns. Two neurones of the suboesophageal ganglion were studied as well as several neurones of thoracic ganglia. The peripheral b...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050970
更新日期:1997-12-01 00:00:00
abstract::Olfactory marker protein (OMP) was first described as a protein expressed in olfactory receptor neurons (ORNs) in the nasal cavity. In particular, OMP, a small cytoplasmic protein, marks mature ORNs and is also expressed in the neurons of other nasal chemosensory systems: the vomeronasal organ, the septal organ of Mas...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03349-9
更新日期:2021-01-15 00:00:00
abstract::The insect midgut epithelium represents an interface between the internal and the external environment and it is the almost unique epithelial tissue by which these arthropods acquire nutrients. This epithelium is indeed able to produce digestive enzymes and to support vectorial transport of small organic nutrients, io...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03076-w
更新日期:2019-09-01 00:00:00
abstract::Brains and subesophageal ganglia from day 3.5 fifth stadium larvae of Manduca sexta were incubated in vitro with 4 nM tritiated ponasterone A, a 20-hydroxyecdysone analog, to determine whether uptake and specific binding of ecdysteroids occur at a cellular level. These tissues, which were taken just prior to the commi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318414
更新日期:1991-01-01 00:00:00
abstract::The effects of radiotherapy on neuropeptide expression in the rat larynx were studied. Irradiation was given for five days, 6 or 8 Gray daily. Ten days after the end of irradiation, the larynx, the laryngeal nerves and different ganglia related to the larynx were dissected out from irradiated and control animals and p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00300691
更新日期:1995-01-01 00:00:00
abstract::Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3 beta-hydroxysteroid dehydrogenase activity and electron microsc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222304
更新日期:1988-08-01 00:00:00
abstract::The use of porcine hepatocytes in xenotransplantation, bioartificial liver support or pharmacological approaches demands serum-free cryopreservation protocols yielding high quality, viable, functional hepatocytes. Here, primary porcine hepatocytes were frozen without serum in liquid nitrogen by the use of a computer-a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0894-6
更新日期:2004-07-01 00:00:00
abstract::Single Golgi impregnated visual cells and their axons were treated from the retina to the first synaptic layer (lamina) in serial electron microscopic sections. This analysis of the retina-lamina projection was undertaken in the upper dorso-median eye region which is known to be involved in the perception of polarized...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218590
更新日期:1975-11-17 00:00:00
abstract::Conventional two-dimensional cell monolayers do not provide the geometrical, biochemical and mechanical cues found in real tissues. Cells in real tissues interact through chemical and mechanical stimuli with adjacent cells and via the extracellular matrix. Such a highly interconnected communication network extends alo...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1589-7
更新日期:2013-04-01 00:00:00
abstract::The objectives of these studies were to determine if (1) hypertrophy-stimulated myogenic regulatory factor (MRF) mRNA increases occur in the absence of proliferating satellite cells, and (2) acute hypertrophy occurs without satellite cell proliferation. Adult and aged quails were exposed to 0 or 2500 Rads gamma irradi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051314
更新日期:1999-06-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. Since its discover...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03287-6
更新日期:2020-10-01 00:00:00