Abstract：
:Male/female differences in enzyme activity and gene expression in the liver are known to be attenuated with ageing. Nevertheless, the effect of ageing on liver structure and quantitative cell morphology remains unknown. Male and female Wistar rats aged 2, 6, 12 and 18 months were examined by means of stereological techniques and immunohistochemical tagging of hepatocytes (HEP), liver sinusoidal endothelial cells (LSEC), Kupffer cells (KC) and hepatic stellate cells (HSC) in order to assess the total number and number per gram of these cells throughout life. The mean cell volume of HEP and HSC, the lobular position and the collagen content of the liver were also evaluated with stereological techniques. The number per gram of HSC was similar for both genders and was maintained throughout ageing. The mean volume of HSC was also conserved but differences in the cell body and lobular location were observed. Statistically significant gender differences in HEP were noted in young rats (females had smaller and more binucleated HEP) but were attenuated with ageing. The same occurred for KC and LSEC, since the higher number per gram in young females disappeared in older animals. Liver collagen increased with ageing but only in males. Thus, the numbers of these four cell types are related throughout ageing, with well-defined cell ratios. The shape and lobular position of HSC change with ageing in both males and females. Gender dimorphism in HEP, KC and LSEC of young rat liver disappears with ageing.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Marcos R,Correia-Gomes Cdoi
10.1007/s00441-016-2490-ysubject
Has Abstractpub_date
2016-12-01 00:00:00pages
639-649issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-016-2490-yjournal_volume
366pub_type
杂志文章abstract：:Two different monoclonal antibodies raised against choline acetyltransferase were used, together with preembedding immunocytochemical techniques, to visualize the possible cholinergic innervation of the supraoptic and paraventricular nuclei of the rat hypothalamus. Light microscopy confirmed the presence of a group of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220024
更新日期：1988-10-01 00:00:00
abstract：:The use of antibodies to alpha-(17--39) ACTH, beta-LPH, and alpha- and beta-endorphins has enabled the detection of immunoreactive cells as early as the eight week of fetal life. Immunoreactive cells are located in the anterior and intermediate lobes of the pituitary gland in normal human fetuses. The same cells react...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225339
更新日期：1978-10-30 00:00:00
abstract：:The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment sh...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225592
更新日期：1989-06-01 00:00:00
abstract：:Calcitonin gene-related peptide-immunoreactive cells were identified within the epithelium of distal conducting airways in the human fetus and infant. Several peptides and amines, including calcitonin, have been identified previously within a specific population of airway epithelial cells. These cells, referred to as ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218949
更新日期：1987-12-01 00:00:00
abstract：:Variations in gene expression patterns represent a powerful source of evolutionary innovation. In a rodent living about 70 million years ago, a genomic accident led an immune formyl peptide receptor (FPR) gene to hijack a vomeronasal receptor regulatory sequence. This gene shuffling event forced an immune pathogen sen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03393-5
更新日期：2021-01-16 00:00:00
abstract：:Caveolae or membrane vesicles are commonly observed in smooth and skeletal muscle as well as in working heart muscle. Using sections of fixed tissue and replicas of freeze-cleaved material, we show in this study that caveolae are also very numerous in sinus node cells of the rabbit, and to a lesser degree, in the atri...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234869
更新日期：1980-01-01 00:00:00
abstract：:Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultrastructural similar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221380
更新日期：1976-10-06 00:00:00
abstract：:Box jellyfish have the most elaborate sensory system and behavioural repertoire of all cnidarians. Sensory input largely comes from 24 eyes situated on four club-shaped sensory structures, the rhopalia, and behaviour includes obstacle avoidance, light shaft attractance and mating. To process the sensory input and conv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0393-7
更新日期：2007-07-01 00:00:00
abstract：:The distribution pattern of adrenergic fibres innervating the ocular choroid membrane of the chicken was studied by means of fluorescence and electron microscopy. In addition, the origin of these fibres was investigated after superior cervical ganglionectomy. Adrenergic axons reach the choroid, partly forming the peri...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213222
更新日期：1982-01-01 00:00:00
abstract：:The ontogeny of protein gene product 9.5 (PGP 9.5), serotonin (5-HT), calcitonin gene-related peptide (CGRP), and calcitonin (CT) immunoreactivity was evaluated in small-granule endocrine cells of hamster laryngotracheal epithelium from fetal day 11 to adulthood. Two centrifugal (proximal-to-distal) patterns of differ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305382
更新日期：1994-01-01 00:00:00
abstract：:The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis vi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0744-z
更新日期：2009-03-01 00:00:00
abstract：:Gastrin stimulates gastric acid secretion by acting on the cholecystokinin B/gastrin receptor (CCK-BR). The localization of this receptor at the cellular level showed conflicting results in animal studies and has not been described in man by immunohistochemistry. The aim of the present study is to characterize the pre...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900114
更新日期：2000-02-01 00:00:00
abstract：:Pax genes are expressed in specific patterns in the nervous system during development and in the adult. Recent findings suggest a link between the expression of Pax-2 and axonal guidance. Mice with a targeted deletion of Pax-2 are an excellent tool for studying axonal pathfinding at the molecular level, especially wit...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050923
更新日期：1997-11-01 00:00:00
abstract：:Interstitial cells of Cajal (ICCs) are believed to be a major element in generating the spontaneous rhythm of the gastrointestinal tract. A prominent problem in the study of these cells has been the difficulty in observing them in intact tissues. We used the lipophilic dye DiI to stain ICCs in the submucosal-circular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051058
更新日期：1998-05-01 00:00:00
abstract：:Cell degeneration, as a phenomenon accompanying developmental processes, was originally described over a century ago. Apoptosis, a term introduced approximately three decades ago, has occupied investigators particularly with respect to cell and tissue kinetics, emphasizing its role in the disposal of supernumerary, ma...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410000227
更新日期：2000-07-01 00:00:00
abstract：:As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233538
更新日期：1981-01-01 00:00:00
abstract：:The structure of the two integumental layers comprising the carapace of female D. magna was examined at several points through the molt cycle. The epicuticle and procuticle are simple in organisation; pore canalsare absent but intracuticular fibres are present, forming complexes with invaginations of the epidermal pla...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214213
更新日期：1976-06-14 00:00:00
abstract：:The present immunocytochemical study concerns the distribution of four neuropeptides, FMRF-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of Diphyllobothrium dendriticum. Anti-FMRF-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the CNS ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222332
更新日期：1985-01-01 00:00:00
abstract：:Many efforts have been made to improve the diagnostic tools used to identify and to estimate the progress of ganglion cell and nerve fibre degeneration in glaucoma. Imaging by optical coherence tomography and measurements of the dimensions of the optic nerve head and the nerve fibre layer in central retinal areas is c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1602-1
更新日期：2013-08-01 00:00:00
abstract：:The hydrozoan medusa Aglantha digitale (Müller 1776) has eight syncytical giant motor axons, up to 40 micrometers in diameter, running from the margin, up the inside of the bell towards the apex. Giant motor axons injected with Lucifer Yellow CH are connected with lateral neurons running circumferentially across the s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258491
更新日期：1982-01-01 00:00:00
abstract：:The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224663
更新日期：1976-08-26 00:00:00
abstract：:The present study attempts to elucidate the role of TRPV1 cation channel receptor on primary repair in an incision-wounded mouse cornea in vivo. Previous study revealed that blocking TRPV1 suppressed myofibroblast formation and expression of transforming growth factor β1 (TGFβ1) in cultured keratocytes or ocular fibro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2878-y
更新日期：2018-11-01 00:00:00
abstract：:The maternal-fetal interface undergoes dynamic changes that promote successful development of the embryo/fetal allograft during pregnancy. This immune privilege of the conceptus is mediated through local and systemic cellular responses. In species in which endometrial decidualization accompanies pregnancy, unique immu...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-015-2315-4
更新日期：2016-01-01 00:00:00
abstract：:To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232246
更新日期：1979-11-01 00:00:00
abstract：:Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217887
更新日期：1983-01-01 00:00:00
abstract：:Neurosecretory pathways were examined in normal male rats by the use of the immunoperoxidase technique for the localization of neurophysin in Bouin-fixed, deparaffinized sections. Using this technique two projections of extrahypothalamic neuorosecretory fibers can be traced to the sites of origin of the choid plexuses...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232829
更新日期：1977-03-01 00:00:00
abstract：:Interstitial cells in the pineal gland of the rat were characterized immunocytochemically using the monoclonal antibodies MRC OX-42 and ED1 for macrophages/microglia, and MRC OX-6, which recognizes major histocompatibility complex (MHC) class II antigen. A polyclonal antibody against GFAP was used to identify astrocyt...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302731
更新日期：1993-05-01 00:00:00
abstract：:This study investigates the relationship between pituitary LHRH responsiveness and the depletion of LH in pubertal rats. The anterior pituitaries of 7-week-old rats of both sexes were stimulated for a maximum of 24 h with either a continuous, or pulsatile exposure to LHRH in vitro. Immunohistochemical examination reve...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218057
更新日期：1986-01-01 00:00:00
abstract：:The startle is a relatively simple ubiquitous reflex. Interestingly, it has a "non-zero baseline", i.e., its magnitude can be reduced or enhanced. We reflect here on the translational value of prepulse inhibition and fear-related potentiation as endophenotypes that can be used for the investigation of complex psychiat...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1599-5
更新日期：2013-10-01 00:00:00
abstract：:The fine structure of boar Leydig cells is re-examined in samples post-fixed with OSO4-K4Fe(CN)6, an elective method to preserve the ultrastructural detail of the endocrine testis. The cells show a pleomorphic arrangement of the smooth endoplasmic reticulum. Large "vesicle-like" dilatations are interspersed in the tub...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00211483
更新日期：1983-01-01 00:00:00
