Abstract:
:Many efforts have been made to improve the diagnostic tools used to identify and to estimate the progress of ganglion cell and nerve fibre degeneration in glaucoma. Imaging by optical coherence tomography and measurements of the dimensions of the optic nerve head and the nerve fibre layer in central retinal areas is currently used to estimate the grade of pathological changes. The visualization and quantification of ganglion cells and nerve fibres directly in patients would dramatically improve glaucoma diagnostics. We have investigated the optical properties of cellular structures of retinal tissue in order to establish a means of visualizing and quantifying ganglion cells in the living retina without staining. We have characterized the optical properties of retinal tissue in several species including humans. Nerve fibres, blood vessels, ganglion cells and their cell processes have been visualized at high image resolution by means of the reflection mode of a confocal laser scanning microscope. The potential of adaptive optics in current imaging systems and the possibilities of imaging single ganglion cells non-invasively in patients are discussed.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Prasse M,Rauscher FG,Wiedemann P,Reichenbach A,Francke Mdoi
10.1007/s00441-013-1602-1subject
Has Abstractpub_date
2013-08-01 00:00:00pages
269-78issue
2eissn
0302-766Xissn
1432-0878journal_volume
353pub_type
杂志文章abstract::The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminifer...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218294
更新日期:1982-01-01 00:00:00
abstract::Three sites of somatostatin-synthesizing perikarya, or a related antigen, were determined by immunofluorescence in the hypothalamus of the tadpole, Alytes obstetricans (Amphibia, Anura). Two sites of neurosecretory perikarya were localized in the preoptic nuclei of the anterior hypothalamus; the axons extended either ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224373
更新日期:1978-02-24 00:00:00
abstract::The gene Ag-Aper14 encodes a novel peritrophic matrix (or peritrophic membrane; PM) protein in the mosquito Anopheles gambiae. The Ag-Aper14 protein is merely 89 amino acids long and has a single putative chitin-binding domain. Prior to blood feeding, the Ag-Aper14 protein is stored in secretory vesicles next to the e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-1067-3
更新日期:2005-04-01 00:00:00
abstract::To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232246
更新日期:1979-11-01 00:00:00
abstract::Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222069
更新日期:1975-01-01 00:00:00
abstract::Receptors for advanced glycation end-products (RAGE) are members of the immunoglobulin superfamily of cell-surface receptors implicated in mechanisms of pulmonary inflammation. In the current study, we test the hypothesis that RAGE mediates inflammation in primary alveolar macrophages (AMs) exposed to diesel particula...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1905-x
更新日期:2014-10-01 00:00:00
abstract::During the reproductive period of the male stickleback, structural and functional changes of the kidney take place, both in the glomeruli and in the renal tubule cells. The structural changes in the glomeruli involve almost all glomerular components and point to a reduction of the glomerular filtration rate. The purpo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210146
更新日期:1981-01-01 00:00:00
abstract::The origin and renewal of the argentaffin cells in the pyloric glands of hamsters were studied by flash, cumulative and pulse labelling autoradiography with 3H-thymidine. The argentaffin cells were identified by the Diazo Method using Fast Red B Salt. By flash labelling autoradiography, it was shown that the argentaff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222771
更新日期:1977-06-20 00:00:00
abstract::The notion that teeth contain stem cells is based on the well-known repairing ability of dentin after injury. Dental stem cells have been isolated according to their anatomical locations, colony-forming ability, expression of stem cell markers, and regeneration of pulp/dentin structures in vivo. These dental-derived s...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-007-0467-6
更新日期:2008-01-01 00:00:00
abstract::The pars tuberalis of the hypophysis of the Djungarian hamster, Phodopus sungorus, was investigated with regard to secretory activity by applying the tannic acid-Ringer perfusion technique. Two groups were maintained under long photoperiods (16 h light: 8 h dark) or short photoperiods (8 h light: 16 h dark), respectiv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00312830
更新日期:1993-08-01 00:00:00
abstract::Fibroblast growth factors (Fgfs) are proteins with diverse functions in development, repair, and metabolism. The human Fgf gene family with 22 members can be classified into three groups, canonical, intracellular, and hormone-like Fgf genes. In contrast to canonical and intracellular Fgfs identified in invertebrates a...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1024-2
更新日期:2010-10-01 00:00:00
abstract::We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within the cells which renders them more detectable, rather than by a real increase in proliferation. The incorporation...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00338049
更新日期:1992-04-01 00:00:00
abstract::Many lung diseases result in fibrotic remodelling. Fibrotic lung disorders can be divided into diseases with known and unknown aetiology. Among those with unknown aetiology, idiopathic pulmonary fibrosis (IPF) is a common diagnosis. Because of its progressive character leading to a rapid decline in lung function, it i...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-016-2543-2
更新日期:2017-03-01 00:00:00
abstract::Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0596-6
更新日期:2008-06-01 00:00:00
abstract::An investigation of the stereospecificity of beta-adrenergic insulin release, its relation to alpha-adrenergic blockade and the adrenergic innervation of the pancreatic islets was performed in the mouse. It was observed that in vivo beta-adrenergic stimulation of insulin release by isopropylnoradrenaline was stereospe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221602
更新日期:1978-10-06 00:00:00
abstract::The three-dimensional architecture of the lamina propria in the jejunal mucosa of the rat was studied by scanning electron microscopy. The lamina propria is a typical reticular tissue with fine nets of reticular fibers and free and fixed cells lying among them. However, the lamina propria in the core of villi displaye...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225563
更新日期:1985-01-01 00:00:00
abstract::The immunological identity of a uteroglobin-like protein, occurring in respiratory tract secretions and tissue, with uteroglobin from rabbit endometrial secretion is demonstrated. A uteroglobin-like antigen has been localized in bronchial epithelial cells and in bronchioles by immunofluorescence. This secretory protei...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210033
更新日期:1978-06-26 00:00:00
abstract::Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410051072
更新日期:1998-06-01 00:00:00
abstract::An immunohistochemical staining technique with the D2-40 antibody was undertaken to examine the functional and morphological features of lymphatic networks in tissue sections and whole-mount preparations of normal nasal mucosa and ethmoid sinus mucosa. In normal nasal mucosa, most lymphatic vessels were found in the s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0337-7
更新日期:2007-03-01 00:00:00
abstract::The responses of non-neuronal cells to the degeneration of neuroblasts were studied in the spinal ganglia of the chick embryo. Reactive changes of the non-neuronal cells were not apparent during the early stage of degeneration of the neuroblast, but during the later stages of degeneration evidence was found suggesting...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210032
更新日期:1978-06-26 00:00:00
abstract::Sox2-expressing stem/progenitor cells in the anterior lobe of the pituitary gland form two types of micro-environments (niches): the marginal cell layer and dense cell clusters in the parenchyma. In relation to the mechanism of regulation of niches, juxtacrine signaling via ephrin and its receptor Eph is known to play...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2646-4
更新日期:2017-10-01 00:00:00
abstract::S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313528
更新日期:1990-09-01 00:00:00
abstract::Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050943
更新日期:1997-11-01 00:00:00
abstract::The effects of light and dark on photoreceptors of the brackish-water polychaete annelid Nereis limnicola were studied by electron microscopy. Animals dark-adapted for one or two days exhibited well-formed straight microvilli (rhabdomeres) on the sensory cell processes. Continuous illumination of worms for one or two ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225427
更新日期:1985-01-01 00:00:00
abstract::The three-dimensional ultrastructure of the pineal body of the rat is described on the basis of freeze-fractured preparations. The pineal capsule consists of irregular cells with very flat and perforated processes. Through these openings, extremely branched canaliculi, extending to almost every pineal cell, communicat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238052
更新日期:1979-09-02 00:00:00
abstract::In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in en...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-02998-9
更新日期:2019-06-01 00:00:00
abstract::Ultrastructural and morphometric investigations were performed on the lung of the European salamander, Salamandra salamandra L. Folds of first and second order are covered with a ciliated epithelium containing goblet cells. The respiratory surface of the lung is lined by a single type of cell which, in amphibians, com...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222429
更新日期:1978-07-27 00:00:00
abstract::The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218204
更新日期:1987-05-01 00:00:00
abstract::The structure of guppy (Poecilia reticulata) spermatogonia and spermatocytes has been studied using electron microscopy. The spermatogonia, situated at the apex of the seminiferous tubule, are almost all surrounded by a network of Sertoli cells; they have very diffuse chromatin and one or two large nucleoli. The cytop...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217139
更新日期:1984-01-01 00:00:00
abstract::The effects of cytochalasin B (CB) on pancreatic structure and amylase release were studied by use of pancreatic fragments, isolated acini and isolated acinar cells. In pancreatic fragments and isolated acini CB caused the disappearance of microfilaments underlying the apical plasma membrane, loss of apical microvilli...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219848
更新日期:1977-04-29 00:00:00