Immunohistochemical evidence for the presence of tryptophan hydroxylase in the brains of insects as revealed by sheep anti-tryptophan hydroxylase polyclonal antibody.

Abstract:

:Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion. In the current study, sheep anti-tryptophan hydroxylase polyclonal antibody has been applied to test for tryptophan hydroxylase immunoreactivity in a broader taxonomic range of insect brains, including holometabolous and hemimetabolous insects: one species each of Coleoptera, Hymenoptera, Diptera, and Blattaria, and two species of Lepidoptera. All species show consistent tryptophan hydroxylase immunoreactivity with distribution patterns matching that of serotonin. The immuno-positive results of such an antibody in brains from diverse orders of insects suggest that specific tryptophan hydroxylase responsible for central serotonin synthesis is probably present in the brains of all insects.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Bao X,Tian X,Zhao Z,Qu Y,Wang B,Zhang J,Liu T,Yang L,Lv J,Song C

doi

10.1007/s00441-008-0596-6

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

555-63

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

332

pub_type

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