Abstract：
:The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment showing MAP2 immunoreactivity. MAP2 immunolabeling was mainly associated associated with filaments which reacted with a monoclonal anti-actin antibody. Also, by immunogold double-labeling we colocalized MAP2 with actin on the endomembranes of the spine apparatus, smooth endoplasmic reticulum, and in the postsynaptic density. Labeling was nearly absent in axons and axonal terminals. These results indicate that MAP2 is an actin-associated protein in dendritic spines. Thus, MAP2 may organize actin filaments in the spine and endow the actin network of the spine with dynamic properties that are necessary for synaptic plasticity.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Morales M,Fifkova Edoi
10.1007/BF00225592subject
Has Abstractpub_date
1989-06-01 00:00:00pages
447-56issue
3eissn
0302-766Xissn
1432-0878journal_volume
256pub_type
杂志文章abstract：:The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223268
更新日期：1975-09-16 00:00:00
abstract：:A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218082
更新日期：1986-01-01 00:00:00
abstract：:Protein kinase C (PKC) is overexpressed in cancer, including pancreatic cancer, compared with normal tissue. Moreover, PKCα is considered one of the biomarkers for the diagnosis of cancers. In several human cancers, the claudin tight junction molecules are abnormally regulated and are thus promising molecular targets ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1287-2
更新日期：2011-12-01 00:00:00
abstract：:The expression of the gene for vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) in the human gastrointestinal tract was studied by in situ hybridization and Northern blotting for PHM/VIP mRNA and immunocytochemistry using specific antisera against the bioactive peptides PHM and VIP. In th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00306108
更新日期：1994-05-01 00:00:00
abstract：:The present immunocytochemical study concerns the distribution of four neuropeptides, FMRF-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of Diphyllobothrium dendriticum. Anti-FMRF-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the CNS ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222332
更新日期：1985-01-01 00:00:00
abstract：:The electric organs of embryonic Torpedo marmorata have been reacted with three cationic stains to evaluate the appearance and distribution of anionic sites. Ruthenium red, alcian blue and lysozyme were used at different pHs and found to react in a time-related manner to anionic components within the interelectrocyte ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214662
更新日期：1987-10-01 00:00:00
abstract：:The effects of high dose injections of corticotropin-releasing hormone (CRH) on the adrenal cortex of hypophysectomized rats were studied at the light- and electron-microscopical levels. Adrenocortical atrophy induced by hypophysectomy could be reduced by daily i.p. injection of 10 micrograms (3 nmol) CRH given for 3 ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297501
更新日期：1990-04-01 00:00:00
abstract：:Aromatase, the enzyme converting androgens into estrogens, is involved in many brain processes such as neural differentiation and plasticity or the prevention of cell death. We have previously observed an increase in aromatase immunoreactivity in sheep neurons exposed in vitro to the oxidant 3-nitro-L: -tyrosine. Howe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1160-3
更新日期：2011-06-01 00:00:00
abstract：:The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time period...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234394
更新日期：1980-01-01 00:00:00
abstract：:The distribution of FMRFamide-like immunoreactive cell bodies and fibers in the nervous system of the earthworm Lumbricus terrestris has been studied by means of immunocytochemistry. The cerebral ganglion contains 150-170 immunoreactive nerve cells that are organized into six major groups in the rostral and five major...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050843
更新日期：1997-06-01 00:00:00
abstract：:Employing electron-microscopic methods that help retain polyanionic materials, we describe the extracellular coverings of a sea urchin (Lytechinus variegatus) throughout ontogeny. The surface of the embryo is covered by a two-layered cuticle (commonly called the hyaline layer), which in turn is covered by a granular l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218028
更新日期：1985-01-01 00:00:00
abstract：:Chondrocytes were isolated enzymatically from rabbit ear cartilage, grown in vitro or as autotransplants for 1, 2 or 5 weeks and then examined by transmission electron microscopy. A confluent monolayer formed rapidly in vitro and the cells later grew in multiple overlapping layers, producing thick sheets of cartilagin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00227600
更新日期：1977-05-31 00:00:00
abstract：:Testicular neoplasms occur spontaneously in androgen insensitive mice with testicular feminization (tfm/y); they are composed of Leydig cells, lipid-laden cells, fibroblastlike cells, and macrophages. The small Leydig cells in the periphery of the tumor are structurally similar to nontumorous tfm/y Leydig cells, where...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234172
更新日期：1980-01-01 00:00:00
abstract：:A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0561-9
更新日期：2008-04-01 00:00:00
abstract：:The developmental mutant of Drosophila (oraJK84) is characterized by nonfunctional photoreceptor cells (R1-6), while the R7/R8 cells are normal. A fundamental question is: Does the near absence of photosensitive membranes inhibit development of the R1-6 axons and their synapses at the other end of the cell? The retina...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238298
更新日期：1983-01-01 00:00:00
abstract：:Quantitative studies on the population of the gonadotropic cells in the pituitary of adult male rats were performed after trypsic dissociation of the pituitary glands and immunoenzymatic staining with anti-beta-LH or anti-beta-FSH antisera. Number, area and extinction of labelled cells were measured by use of an image...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217273
更新日期：1982-01-01 00:00:00
abstract：:The effects of dexamethasone on the ability of cells enzymatically isolated from 21-day fetal rat calvaria to produce cartilage in vitro has been investigated. Primary cultures of single-cell suspensions of rat calvaria were grown for up to 28 days in vitro in alpha-minimal essential medium containing 15% fetal bovine...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224728
更新日期：1989-04-01 00:00:00
abstract：:The presence of immunoreactive enkephalin, dynorphin, vasoactive intestinal polypeptide, cholecystokinin, substance P and neuropeptide Y in nerve fibers that project to the guinea-pig inferior mesenteric ganglion was analysed, after different denervation and ligation procedures. A quantitative analysis demonstrates th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219741
更新日期：1988-09-01 00:00:00
abstract：:Acute necrotizing pancreatitis (ANP) is a common gastrointestinal cause of emergency admissions in dogs and humans and can lead to a systemic inflammatory response syndrome resulting in multiple organ dysfunction syndrome. Among the various complications associated with ANP, acute lung injury (ALI) or acute respirator...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03211-y
更新日期：2020-05-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP) is the latest member of the vasoactive intestinal polypeptide (VIP) family of neuropeptides present in nerve fibres in many peripheral organs. Using double immunohistochemistry, with VIP as a marker for intrinsic innervation and calcitonin-gene related peptide...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900124
更新日期：2000-01-01 00:00:00
abstract：:More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217555
更新日期：1985-01-01 00:00:00
abstract：:The periodontal ligament (PDL) is a highly specialized tissue connecting the cementum with the tooth socket bone and affects the life span of the tooth. However, little is known about the precise characteristics and regenerative mechanism of PDL cells because of the absence of specific markers and cell lines. Therefor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0101-4
更新日期：2006-04-01 00:00:00
abstract：:Serine proteases, cysteine proteases, aspartic proteases and matrix metalloproteinases play an essential role in extracellular matrix remodeling and turnover through their proteolytic action on collagens, proteoglycans, fibronectin, elastin and laminin. Proteases can also act on chemokines, receptors and anti-microbia...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1390-z
更新日期：2013-02-01 00:00:00
abstract：:(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209140
更新日期：1978-06-08 00:00:00
abstract：:Aquaporins (AQPs) are integral membrane proteins that function as channels for the transfer of water and small solutes across membranes. In mammalian cells, 13 isoforms (AQP0-12) have been identified, and these exhibit unique patterns of expression in various cell types and tissues. Among these isoforms, AQP6 is consi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0558-4
更新日期：2008-04-01 00:00:00
abstract：:Rhabdomeres of the Drosophila melanogaster canoemisl mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900107
更新日期：1999-12-01 00:00:00
abstract：:Neuropeptide F in invertebrates is a homolog of neuropeptide Y in mammals and it is a member of FMRFamide-related peptides. In arthropods, such as insects, there are two types of neuropeptide F comprising long neuropeptide F (NPF) and short neuropeptide F (sNPF). Both NPFs are known to play a crucial role in the regul...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-02999-8
更新日期：2019-06-01 00:00:00
abstract：:The three-dimensional ultrastructure of the pineal body of the rat is described on the basis of freeze-fractured preparations. The pineal capsule consists of irregular cells with very flat and perforated processes. Through these openings, extremely branched canaliculi, extending to almost every pineal cell, communicat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238052
更新日期：1979-09-02 00:00:00
abstract：:Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only ca...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100395
更新日期：2001-07-01 00:00:00
abstract：:The therapeutic potency of delivered mesenchymal stem cells (MSCs) in tissue engineering applications may be improved by priming cells toward a differentiated state via coculture with native, differentiated cells prior to implantation; however, there is a lack of understanding in what may be the most efficacious metho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1265-8
更新日期：2012-03-01 00:00:00
