Abstract:
:The ontogeny of two gonadotropin-releasing-hormone (GnRH) systems, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), was investigated in zebrafish (Danio rerio). In situ hybridization (ISH) first detected sGnRH mRNA-expressing cells at 1 day post-fertilization (pf) anterior to the developing olfactory organs. Subsequently, cells were seen along the ventral olfactory organs and the olfactory bulbs, reaching the terminal nerve (TN) ganglion at 5-6 days pf. Some cells were detected passing posteriorly through the ventral telencephalon (10-25 days pf), and by 25-30 days pf, sGnRH cells were found in the hypothalamic/preoptic area. Continuous documentation in live zebrafish was achieved by a promoter-reporter expression system. The expression of enhanced green fluorescent protein (EGFP) driven by the sGnRH promoter allowed the earlier detection of cells and projections and the migration of sGnRH neurons. This expression system revealed that long leading processes, presumably axons, preceded the migration of the sGnRH neuron somata. cGnRH-II mRNA expressing cells were initially detected (1 day pf) by ISH analysis at lateral aspects of the midbrain and later on (starting at 5 days pf) at the midline of the midbrain tegmentum. Detection of red fluorescent protein (DsRed) driven by the cGnRH-II promoter confirmed the midbrain expression domain and identified specific hindbrain and forebrain cGnRH-II-cells that were not identified by ISH. The forebrain DsRed-expressing cells seemed to emerge from the same site as the sGnRH-EGFP-expressing cells, as revealed by co-injection of both constructs. These studies indicate that zebrafish TN and hypothalamic sGnRH cell populations share a common embryonic origin and migratory path, and that midbrain cGnRH-II cells originate within the midbrain.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Palevitch O,Kight K,Abraham E,Wray S,Zohar Y,Gothilf Ydoi
10.1007/s00441-006-0279-0subject
Has Abstractpub_date
2007-02-01 00:00:00pages
313-22issue
2eissn
0302-766Xissn
1432-0878journal_volume
327pub_type
杂志文章abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2857-3
更新日期:2018-10-01 00:00:00
abstract::The distribution of radioactivity in the rat hypothalamus after intraventricular injection of tritiated luteinizing hormone-releasing hormone (LH-RH) was studied by autoradiography and compared with that expected from Fick's second law which defines the character of ordinary diffusion. The results suggest that LH-RH p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215317
更新日期:1976-05-13 00:00:00
abstract::Bone morphogenetic proteins (BMPs) induce cartilage and bone formation at both bony and non-bony sites. We examined the possibility whether BMP-2 induces differentiation of osteoblast progenitors into chondroblast lineage cells using organ culture and cell culture prepared from the calvaria of newborn mouse. BMP-2 sti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050562
更新日期:1996-04-01 00:00:00
abstract::Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209276
更新日期:1978-05-29 00:00:00
abstract::Exposure of adult brown bullheads Ictalurus nebulosus (120-450 g) to environmental hypercapnia (2% carbon dioxide in air) and subsequent recovery caused transient changes in whole body net sodium flux (JnetNa+) and net chloride flux (JnetCl-) resulting largely from changes in whole body sodium influx (JNa+in) and chlo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319161
更新日期:1992-06-01 00:00:00
abstract::The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225806
更新日期:1988-11-01 00:00:00
abstract::The effects of light and dark on photoreceptors of the brackish-water polychaete annelid Nereis limnicola were studied by electron microscopy. Animals dark-adapted for one or two days exhibited well-formed straight microvilli (rhabdomeres) on the sensory cell processes. Continuous illumination of worms for one or two ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225427
更新日期:1985-01-01 00:00:00
abstract::Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only ca...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100395
更新日期:2001-07-01 00:00:00
abstract::The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminifer...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218294
更新日期:1982-01-01 00:00:00
abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238295
更新日期:1983-01-01 00:00:00
abstract::Sections of glutaraldehyde-OsO4-fixed, plastic-embedded rat incisor enamel were left untreated, stained, decalcified (1% formic acid in 10% sodium citrate), or decalcified-stained. The presence of apatite crystals was monitored with electron diffraction. After brief decalcification and staining, apatite crystals and m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215908
更新日期:1986-01-01 00:00:00
abstract::Early in postnatal life, the first wave of spermatogenesis is accompanied by an initial wave of germ cell apoptosis. This may reflect an adjustment in the number of germ cells that can be adequately maintained by Sertoli cells. Two major pathways (intrinsic and extrinsic) are involved in the process of caspase activat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0186-4
更新日期:2006-09-01 00:00:00
abstract::Stellate cells in the rabbit adenohypophysis were observed electron microscopically under normal and experimental conditions such as lactation, thyroidectomy, adrenalectomy, or castration. In control animals stellate cells had a scanty cytoplasm surrounding the nucleus and possessed slender processes extending between...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234784
更新日期:1980-01-01 00:00:00
abstract::A body of evidence points to the existence of stem cell stores in adult tissues, in addition to the well-known hematopoietic stem cells from bone marrow. Many reports describe the ability of these multipotent cells (developmentally non-compromised with their organs of origin) to give rise to many different cell types ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-006-0172-x
更新日期:2006-09-01 00:00:00
abstract::The meninges of albino Wistar rat embryos, aged between the 11th embryonic day (ED) and birth, were sectioned using a specially constructed device. This technique permits optimal microanatomical preservation of all tissues covering the convexity of the brain: skin, muscle, cartilage or bone, and the meninges. At ED11,...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221652
更新日期:1989-07-01 00:00:00
abstract::A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytoc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050884
更新日期:1997-08-01 00:00:00
abstract::The thyroid cells of the cream hamster, characterized by abundance of microtubules and stratification of the organelles, undergo a particular evolution when the animals grow older. These changes are characterized by an increase of the number of lysosomes which in extreme cases become so prominent that they occupy the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215124
更新日期:1976-02-06 00:00:00
abstract::The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicle...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100351
更新日期:2001-04-01 00:00:00
abstract::The accessory medulla with its associated pigment-dispersing hormone-immunoreactive neurons appears to be the pacemaker that controls the circadian locomotor activity rhythm of the cockroach Leucophaea maderae. To permit studies at the level of individual, identified, pacemaker neurons, we developed specific long-term...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051324
更新日期:1999-06-01 00:00:00
abstract::The distribution and ultrastructure of the mitochondria-rich (MR) cells in the gills of larval (ammocoetes) and adult lampreys (Petromyzon marinus and Geotria australis) have been studied. One type of MR cell, which is found only in ammocoetes, occurs in groups on and between gill lamellae. Freeze-fracture replicas sh...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051003
更新日期:1998-02-01 00:00:00
abstract::The use of porcine hepatocytes in xenotransplantation, bioartificial liver support or pharmacological approaches demands serum-free cryopreservation protocols yielding high quality, viable, functional hepatocytes. Here, primary porcine hepatocytes were frozen without serum in liquid nitrogen by the use of a computer-a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0894-6
更新日期:2004-07-01 00:00:00
abstract::Rat ovarian perifollicular contractile tissue was examined at specified intervals prior to ovulation to determine the type, relative number, and length of cellular junctions. Rat ovaries were taken for electron-microscopic observation at 1500 h on the afternoon of proestrus (proestrus 0-h group), at 2000 h (proestrus ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210023
更新日期:1981-01-01 00:00:00
abstract::It is believed that differentiation and maintenance of taste buds in vertebrates is dependent on the trophic function of their sensory nerve supply. In the present work, colchicine was injected into the circumvallate papilla of the rat. This produced a reversible blockade of neuroplasmic transport and disappearance of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00231023
更新日期:1978-08-25 00:00:00
abstract::The innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (Tranzer and Richards, 1976). Three types of nerve profiles were revealed: (1) 60-70% of the profiles are adrenergic; (2) less than 5% o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236418
更新日期:1979-08-01 00:00:00
abstract::There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2612-1
更新日期:2017-08-01 00:00:00
abstract::The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0667-8
更新日期:2008-10-01 00:00:00
abstract::Dexamethasone (Dex) regulates osteoblastic and adipocytic differentiation in mesenchymal progenitor cells through regulation of Wnt/β-catenin signaling. To elucidate the regulatory mechanisms underlying the effects of Dex, we examine the expression of Axin2, which is an intracellular inhibitor of Wnt/β-catenin signali...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1696-5
更新日期:2013-12-01 00:00:00
abstract::Injection of wheat-germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the superior cervical ganglion (SCG) of the rat results in accumulation of WGA-HRP in sympathetic postganglionic neurons in the contralateral SCG. The sympathetic pathways involved and the mechanism underlying the labeling were investig...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00327745
更新日期:1990-10-01 00:00:00
abstract::The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03197-7
更新日期:2020-11-01 00:00:00
abstract::We have analyzed the distribution of neural crest-derived precursors and the expression of catecholaminergic and neuronal markers in developing adrenal tissue of chick embryos. Undifferentiated neural crest cells are found in presumptive adrenal regions from embryonic day 3 (E3) onward. An increasing proportion of cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0996-1
更新日期:2005-01-01 00:00:00