Abstract：
:Intracellular insulin-binding sites were directly traced in fixed monolayer cultures of a variety of cell types with the use of two fluorescent derivatives of insulin, viz. fluorescein isothiocyanate (FITC)-labelled and tetramethyl rhodamine isothiocyanate (TMRITC)-labelled insulin. Both derivatives retained the property of stimulating DNA synthesis in fibroblasts. Insulin-binding sites were found in the nuclear envelope, nucleoplasm, nucleoli, and in mitochondria and rough endoplasmic reticulum. The identity of these structures was established by concomitant studies on the same cell by means of phase contrast optics and immunocytochemical tracing with specific antibodies to nuclei, mitochondria, or ribosomes. Binding of insulin to the nuclear and cytoplasmic structures was rapid, reversible and saturable, temperature and pH-dependent, and inhibited by an excess of native, but not other, hormones. The staining reactions were sensitive to treatment by the nonionic detergents, NP-40 and TX-100, and to trypsin and pronase, but not to DNase and RNase, suggesting that the binding sites are protein in nature.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Lolait SJ,Toh BHdoi
10.1007/BF00232150subject
Has Abstractpub_date
1980-01-01 00:00:00pages
145-53issue
1eissn
0302-766Xissn
1432-0878journal_volume
210pub_type
杂志文章abstract：:Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1113-2
更新日期：2011-03-01 00:00:00
abstract：:Effects of steroids on the accumulation of glycoprotein gonadotropin (GTH) in pituitaries of juvenile trout were investigated by means of scanning cytophotometry applied to immunocytochemical preparations, and with the use of a radioimmunoassay. Effects on other aspects of GTH-cell activity were analyzed by measuring ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226962
更新日期：1984-01-01 00:00:00
abstract：:Rat ovarian perifollicular contractile tissue was examined at specified intervals prior to ovulation to determine the type, relative number, and length of cellular junctions. Rat ovaries were taken for electron-microscopic observation at 1500 h on the afternoon of proestrus (proestrus 0-h group), at 2000 h (proestrus ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210023
更新日期：1981-01-01 00:00:00
abstract：:The otoliths of embryos and young animals of the lizard Podarcis s. sicula were studied by X-ray diffraction and scanning electron microscopy. Two types of crystal that give different X-ray diffraction patterns were found in the membranous labyrinth of Podarcis. The crystals consist of calcite or aragonite and are eas...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210342
更新日期：1981-01-01 00:00:00
abstract：:Synaptogyrins are conserved components of the exocytic apparatus and function as regulators of Ca(2+)-dependent exocytosis. The synaptogyrin family comprises three isoforms: two neuronal (synaptogyrin-1 and -3) and one ubiquitous (synaptogyrin-2) form. Although the expression patterns of the exocytic proteins synaptot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1629-3
更新日期：2013-09-01 00:00:00
abstract：:Because leucokinins stimulate diuresis in some insects, we wished to identify the neurosecretory cells in Manduca sexta that might be a source of leucokinin-like neurohormones. Immunostaining was done at various stages of development, using an antiserum to leucokinin IV. Bilateral pairs of neurosecretory cells in abdo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331367
更新日期：1994-12-01 00:00:00
abstract：:Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050943
更新日期：1997-11-01 00:00:00
abstract：:The system of lateral-line organs in urodeles was examined by the use of various light- and electron-microscopical techniques. The results show that, in addition to the well-known mechanoreceptive neuromast organs, a second type of receptor can be identified. This second type of organ was presumably seen by earlier wo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00207693
更新日期：1983-01-01 00:00:00
abstract：:Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug. In the distal elongating tips of myotubes, microtubules were the major cytoskeletal el...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214368
更新日期：1988-05-01 00:00:00
abstract：:Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222069
更新日期：1975-01-01 00:00:00
abstract：:Sex pheromones orient male moths toward conspecific female moths; the presence of visual information modulates this behavior. In the current study, we explore candidate neuronal pathways for the interaction between vision and the locomotor signal for pheromone orientation. We describe the connectivity between visual n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2892-0
更新日期：2018-12-01 00:00:00
abstract：:During the first postnatal weeks of the developing rodent retina, rhodopsin can be detected in a number of neuron-like cells in the inner retina. In the present study, we aim to characterize the morphology, number and staining characteristics of this peculiar population. Misplaced rhodopsin-positive cells (MRCs) were ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1788-2
更新日期：2014-04-01 00:00:00
abstract：:Previous studies have indicated that 19-day-old fetal long bones of the rat contain an adenylyl cyclase-stimulating activity antigenically related to parathyroid hormone-related peptide. To ascertain its origin, Northern blotting and in situ hybridization histochemistry were performed. Results demonstrate that mRNA of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645063
更新日期：1992-12-01 00:00:00
abstract：:An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect specie...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050601
更新日期：1996-06-01 00:00:00
abstract：:Fibres growing from neurons of explanted dorsal root ganglia from 10 day chick embryos were transected and subsequently observed by light and electron microscopy after periods of a few to fifty minutes. Changes immediately proximal and distal to the cut together with alterations further away from the site of injury on...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219562
更新日期：1978-07-10 00:00:00
abstract：:Peeling skin syndrome is a heterogeneous group of rare disorders. Peeling skin, leukonychia, acral punctate keratoses, cheilitis and knuckle pads (PLACK syndrome, OMIM616295) is a newly described form of PSS with an autosomal recessive mode of inheritance. We report a 5.5-year-old boy with features of PLACK syndrome. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03077-9
更新日期：2019-11-01 00:00:00
abstract：:Pituitaries from normal, young and adult male rats were fixed either in sublimate-formalin or in glutaraldehyde-osmium. In adjacent Paraplast sections, almost all the gonadotrophs were immunostained with both LH and FSH antisera. The rat LH beta and FSH antisera used were shown to be highly specific by the absorption ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233820
更新日期：1981-01-01 00:00:00
abstract：:Eosinophils are innate immune leukocytes found in relatively low numbers within the blood. Terminal effector functions of eosinophils, deriving from their capacity to release their content of tissue-destructive cationic proteins, have historically been considered primary effector mechanisms against specific parasites,...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1049-6
更新日期：2011-01-01 00:00:00
abstract：:We previously described the presence of MHC class II (HLA-DR) antigens, structurally similar to those on lymphoid cells and bearing the genetically-appropriate allotypic determinants, on human adrenocortical cells in the zona reticularis of normal glands. We now report a similar expression by granulosa-lutein cells (G...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00309876
更新日期：1990-11-01 00:00:00
abstract：:The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particul...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213955
更新日期：1986-01-01 00:00:00
abstract：:Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1885-x
更新日期：2014-06-01 00:00:00
abstract：:The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Im...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1110-z
更新日期：2005-07-01 00:00:00
abstract：:The effects of thyroidectomy, adrenalectomy, and castration on the pars distalis of male Japanese quail, and of injection of LH-RH on sexually inactive females, were investigated by light and electron microscopy. Correlation between light and electron microscopy was attained by use of alternate thin and thick sections...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219152
更新日期：1975-06-09 00:00:00
abstract：:The use of antibodies to alpha-(17--39) ACTH, beta-LPH, and alpha- and beta-endorphins has enabled the detection of immunoreactive cells as early as the eight week of fetal life. Immunoreactive cells are located in the anterior and intermediate lobes of the pituitary gland in normal human fetuses. The same cells react...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225339
更新日期：1978-10-30 00:00:00
abstract：:Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0480-9
更新日期：2008-01-01 00:00:00
abstract：:The startle is a relatively simple ubiquitous reflex. Interestingly, it has a "non-zero baseline", i.e., its magnitude can be reduced or enhanced. We reflect here on the translational value of prepulse inhibition and fear-related potentiation as endophenotypes that can be used for the investigation of complex psychiat...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1599-5
更新日期：2013-10-01 00:00:00
abstract：:Prostatic carcinoma affects 1 in 11 men and targets bone with sclerotic metastases. The study of prostate carcinoma growth in bone has been hampered by the lack of suitable animal models. We have developed an in vivo model of prostate carcinoma growth in bone by inoculating three human prostate carcinoma cell lines (P...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-001-0503-x
更新日期：2002-03-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:The course of the Nervus connectivus (N.c.), its branches, and synaptic connections within the frontal ganglion (FG) were investigated electron microscopically after cobalt iontophoresis of the N.c. The subsequent treatment of ultrathin sections with Timm's method was found to be very suitable for identifying the smal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235172
更新日期：1979-01-01 00:00:00
abstract：:Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their periphera...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03199-5
更新日期：2020-06-01 00:00:00
