Abstract:
:The present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. A marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed on the basis of changes in binding patterns of the following fluoroscein-isothiocyanate (FITC)-labelled lectins: wheat-germ agglutinin (WGA), Maclura pomifera agglutinin (MPA), Griffonia simplicifolia I agglutinin (GS I) and Bauhinea purpurea agglutinin (BPA). This was evidenced in the first three cases by a relative reversal of staining intensity between the acrosomal and post-acrosomal regions, and by a pronounced increase in the staining of the midpiece. Changes in the distribution of BPA binding sites were limited to the latter phenomenon. The results are compared with previous findings, discussed in the context of the hypermotility characteristic of ampullary sperm and related to previously reported differences in the lectin-binding patterns of the luminal and glandular epithelia.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Bergerson W,Amselgruber W,Sinowatz F,Bergerson Mdoi
10.1007/BF00318822subject
Has Abstractpub_date
1994-03-01 00:00:00pages
537-41issue
3eissn
0302-766Xissn
1432-0878journal_volume
275pub_type
杂志文章abstract::The intrapancreatic localization and the effects on basal and stimulated insulin secretion of neuropeptide Y (NPY) were investigated in the mouse. Immunocytochemistry showed NPY to be confined to intrapancreatic nerve fibers mainly associated with blood vessels. Fine varicose NPY fibers were also detected in the exocr...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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更新日期:1999-07-01 00:00:00