Abstract：
:Electron-dense granules (EDGs) are singular structures found in the tissues of several vertebrate and invertebrate organisms. Two types of EDGs were observed in hepatopancreatic cells of the crab Ucides cordatus: (1) a non-mineralized EDG, found mainly inside vacuoles, which reacted positively to acid phosphatase and D-amino acid oxidase, possibly formed by degradation of lipid membranes, and (2) a mineralized EDG surrounded by endoplasmic reticulum membranes that gave a positive reaction for glucose-6-phosphatase. In this study we show the fine structure and composition of the mineralized EDGs using cytochemistry, analytical transmission electron microscopy and field-emission scanning electron microscopy. They are formed of microvesicle-like structures that are arranged in concentric spherical layers in the most mineralized portions of the granule. Analytical microscopy of mineralized EDGs indicated that they are composed of amorphous calcium-magnesium phosphate. Isolated EDGs treated with NaOCl lose several elements, including P, when compared with EDGs treated with deionized water. Removal of the organic matrix by NaOCl induced marked changes in the mineralized EDGs, showing that the organic matrix plays an important role in its elemental composition and structure.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Corrêa Junior JD,Allodi S,Farina Mdoi
10.1007/s00441-002-0646-4keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
107-16issue
1eissn
0302-766Xissn
1432-0878journal_volume
311pub_type
杂志文章abstract：:A combination of light, transmission and scanning electron microscopy was employed to demonstrate the occurrence, arrangement and structure of taste buds in the oral mucosa of the soft palate of monkeys (Macaca irus). Taste buds are found in aggregates confined to 0.15 to 0.3 mm wide, round islands of keratinizing epi...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1984-01-01 00:00:00
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更新日期：1976-03-05 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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更新日期：1984-01-01 00:00:00
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