Abstract:
:Transforming growth factor beta (TGF-beta) is a major member of a cytokine family with pluripotent biological activity. In the liver, TGF-beta has pathophysiological significance with respect to hepatic fibrogenesis, regulation of liver cell growth, tumor development, and induction of hepatocellular apoptosis. We show that the expression of immunocytochemically detectable TGF-beta in cultured hepatocytes is strongly dependent on culture conditions and cellular microenvironment. Hepatocytes in situ and freshly isolated cells are TGF-beta negative. In contrast, hepatocytes in incubated liver slices are stained with a patch-like pattern, whereas parenchymal cells cultured as a monolayer exhibit strong diffuse positive immunostaining for TGF-beta within 2 h after seeding. The intensity of immunocytochemical staining is dependent on culture substrata, major expression being observed in cells maintained on glass or plastic surfaces. When collagen type-I and type-IV, or EHS-matrix are used as substrata, TGF-beta is absent or only weakly immunocytochemically apparent in cultured hepatocytes, irrespective of the culture medium used. The positive immunocytochemical expression of TGF-beta in hepatocytes arises neither by transcriptional and translational pathways nor by disturbed intracellular calcium homeostasis. However, calcium-dependent proteinases, such as calpain-I and -II, might be involved in the immunochemical presentation of intracellular TGF-beta, because respective calpain inhibitors strongly reduce the appearance of TGF-beta in cultured parenchymal liver cells. Thus, hepatocytes are a major cellular source of (latent) TGF-beta in liver, as becomes evident during culture.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Gressner AM,Wulbrand Udoi
10.1007/s004410050740subject
Has Abstractpub_date
1997-01-01 00:00:00pages
143-52issue
1eissn
0302-766Xissn
1432-0878journal_volume
287pub_type
杂志文章abstract::The LIM homeodomain transcription factor Lmx1a shows a dynamic expression in the developing mouse ear that stabilizes in the non-sensory epithelium. Previous work showed that Lmx1a functional null mutants have an additional sensory hair cell patch in the posterior wall of a cochlear duct and have a mix of vestibular a...
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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