Expression of Slc35f1 in the murine brain.

Abstract:

:The solute carrier (SLC) group of membrane transport proteins includes about 400 members organized into more than 50 families. The SLC family that comprises nucleoside-sugar transporters is referred to as SLC35. One of the members of this family is SLC35F1. The function of SLC35F1 is still unknown; however, recent studies demonstrated that SLC35F1 mRNA is highly expressed in the brain and in the kidney. Therefore, we examine the distribution of Slc35f1 protein in the murine forebrain using immunohistochemistry. We could demonstrate that Slc35f1 is highly expressed in the adult mouse brain in a variety of different brain structures, including the cortex, hippocampus, amygdala, thalamus, basal ganglia, and hypothalamus. To examine the possible roles of Slc35f1 and its subcellular localization, we used an in vitro glioblastoma cell line expressing Slc35f1. Co-labeling experiments were performed to reveal the subcellular localization of Slc35f1. Our results indicate that Slc35f1 neither co-localizes with markers for the Golgi apparatus nor with markers for the endoplasmic reticulum. Time-lapse microscopy of living cells revealed that Slc35f1-positive structures are highly dynamic and resemble vesicles. Using super-resolution microscopy, these Slc35f1-positive spots clearly co-localize with the recycling endosome marker Rab11.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Farenholtz J,Artelt N,Blumenthal A,Endlich K,Kroemer HK,Endlich N,von Bohlen Und Halbach O

doi

10.1007/s00441-019-03008-8

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

167-176

issue

2

eissn

0302-766X

issn

1432-0878

pii

10.1007/s00441-019-03008-8

journal_volume

377

pub_type

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