Abstract：
:The ultrastructure of crystalline beta granules of the islets of Langerhans in the alligator has been investigated. From optical diffraction analysis and serial sectioning, the existence of four distinct types of crystalline inclusions was established in ultrathin sections. The first type is the most frequent and is interpreted as a rhombohedron with a base, the ortho-hexagonal unit-cell edges being a equal to 18.9 nm, c equal to 23.0 nm. The second type of crystal (not observed in serial sections) is found compatible with a rhomb-dodecahedron which indexes on a cubic cell with a equal to 9.6 nm. The third type of crystal was assigned to dipyramids. Dipyramids are extremely rare, and only two diffraction patterns were obtained; their crystal system could not be determined. Prisms, which are second in abundance, represent the fourth type of crystal. Spacings as well as the symmetry differ from those of the above three crystal types and indicate a tetragonal cell with a equal to 4.2 nm, c equal to 14.2 nm. The data for the prismatic crystals are strikingly similar to those of proinsulin and may represent the first case of agreement between crystals (i) formed in vitro and studied by X-ray diffraction and (ii) those investigated in situ by electron microscopy.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Raska I,Komrska J,Titlbach M,Rieder Mdoi
10.1007/BF00229618subject
Has Abstractpub_date
1978-03-13 00:00:00pages
535-50issue
3eissn
0302-766Xissn
1432-0878journal_volume
187pub_type
杂志文章abstract：:Retinae of Abyssinian cats homozygous for a retinal degeneration gene, and normal controls, have been investigated using antibodies directed against opsin, transducin alpha (TD-alpha), S-antigen (48K protein), interphotoreceptor retinoid-binding protein (IRBP), and cone outer segments. IRBP-immunoreactivity (IR) is mu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221474
更新日期：1989-09-01 00:00:00
abstract：:Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2726-5
更新日期：2018-02-01 00:00:00
abstract：:Whether environmental exposure to bisphenol A (BPA) may induce reproductive disorders is still controversial but certain studies have reported that BPA may cause meiotic abnormalities in C. elegans and female mice. However, little is known about the effect of BPA on meiosis in adult males. To determine whether BPA exp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1723-6
更新日期：2014-01-01 00:00:00
abstract：:The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-act...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215699
更新日期：1982-01-01 00:00:00
abstract：:The isolated perfused heart model was used to examine the structure of rat cardiac capillaries and their permeability to macromolecules of various sizes. Haemoglobin (diameter 6.4 nm) and catalase (10.4 nm) did not cross the endothelium but remained on the luminal side. Cytochrome C (3 nm) and horseradish peroxidase (...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213826
更新日期：1988-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0036-9
更新日期：2005-12-01 00:00:00
abstract：:Ornithine decarboxylase (ODC) activity was measured in homogenates of neonatal rabbit ovaries from the day of birth to day 22, an interval that corresponds to the progressing stages of the meiotic prophase from oogonia to diplotene and follicle formation. The ODC-activity was high on days 1 and 3 when most germ cells ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218114
更新日期：1983-01-01 00:00:00
abstract：:Developmental alterations of paranodal fiber segments have not been investigated systematically in human nerve fibers at the light- and electron-microscopic level. We have therefore analyzed developmental changes in the fine structure of the paranode in 43 human sural nerves during the axonal growth period up to 5 yea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00333704
更新日期：1993-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219856
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00309876
更新日期：1990-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319432
更新日期：1994-02-01 00:00:00
abstract：:The dental follicle is an ectomesenchymal tissue that surrounds developing tooth germ and that contains osteoblastic-lineage-committed stem/progenitor cells. We examined the osteogenic potential of human dental follicle cells (hDFC) by microarray analysis. We first compared the characteristics of hDFC with those of hu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1477-6
更新日期：2012-11-01 00:00:00
abstract：:The spleen of the oriental fire-bellied toad, Bombina orientalis, consists of well-developed white pulp, separated from the lymphocytic marginal zone by the connective tissue boundary layer. Injection of peroxidase-conjugated rabbit anti-peroxidase revealed that these immune complexes were localized on the surface of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297553
更新日期：1993-01-01 00:00:00
abstract：:During the first postnatal weeks of the developing rodent retina, rhodopsin can be detected in a number of neuron-like cells in the inner retina. In the present study, we aim to characterize the morphology, number and staining characteristics of this peculiar population. Misplaced rhodopsin-positive cells (MRCs) were ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1788-2
更新日期：2014-04-01 00:00:00
abstract：:TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and seem to influence the rheological properties of mucous gels. Here, localization studies of TFF-peptides in human salivary glands are presented. Expression studies (polymerase chain reaction) revealed ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900087
更新日期：1999-10-01 00:00:00
abstract：:Prostatic carcinoma affects 1 in 11 men and targets bone with sclerotic metastases. The study of prostate carcinoma growth in bone has been hampered by the lack of suitable animal models. We have developed an in vivo model of prostate carcinoma growth in bone by inoculating three human prostate carcinoma cell lines (P...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-001-0503-x
更新日期：2002-03-01 00:00:00
abstract：:The transient receptor potential (TRP) superfamily comprises a group of non-selective cation channels that have been implicated in both receptor and store-operated channel functions. The family of the classical TRPs (TRPCs) consists of seven members (TRPC1-7). The presence of TRPC1 and TRPC5 mRNA in the brain has prev...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0004-4
更新日期：2005-11-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:The colocalization of immunoreactivities to substance P and calcitonin gene-related peptide (CGRP) in nervous structures and their correlation with other peptidergic structures were studied in the stellate ganglion of the guinea pig by the application of double-labelling immunofluorescence. Three types of fibre were d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318563
更新日期：1993-06-01 00:00:00
abstract：:The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2127-6
更新日期：2015-04-01 00:00:00
abstract：:The olfactory epithelium of mice after axotomy was investigated to clarify the stem cells of olfactory cells by double immunostaining using antikeratin (MA903) and anti-bromodeoxyuridine (BrdU) antibodies and by conventional electron microscopy. When a single dose of BrdU was given to mice 9 days after axotomy, immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318179
更新日期：1991-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00304610
更新日期：1993-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/BF00215904
更新日期：1986-01-01 00:00:00
abstract：:Kruppel-like factor 4 (KLF4) is a zinc-finger-type transcription factor with a restricted expression pattern during skeletal development. We have previously shown that KLF4 represses osteoblast mineralization concomitant with a down-regulation in the expression of a number of osteoblastic genes, both in vivo and in vi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1931-8
更新日期：2014-10-01 00:00:00
abstract：:To investigate the role of RhoA on the intracellular membrane dynamics of lysosomes in rat hepatoma cells (MM1), we analyzed the localization of lysosomal aspartic proteinase cathepsin D by confocal immunofluorescence microscopy in the dominant active RhoA-transfected cells. Here we show that the transfection of the d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051032
更新日期：1998-04-01 00:00:00
abstract：:The ontogeny of gut innervation in the anuran amphibian Xenopus laevis was studied using immunohistochemistry on sections of whole larvae from NF stages 38-52. Immunoreactivity to acetylated tubulin confirmed the presence of nerve fibres as early as stages 38-39. Actin immunoreactivity was found at stage 41, indicatin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100423
更新日期：2001-10-01 00:00:00
abstract：:Variations in gene expression patterns represent a powerful source of evolutionary innovation. In a rodent living about 70 million years ago, a genomic accident led an immune formyl peptide receptor (FPR) gene to hijack a vomeronasal receptor regulatory sequence. This gene shuffling event forced an immune pathogen sen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03393-5
更新日期：2021-01-16 00:00:00
abstract：:The distribution and chemical neuroanatomy of nervous elements and certain pharmacological-physiological characteristics of the innervation of the body wall in earthworms are described. Solitary sensory bipolar cells can be found among the epithelial cells. These bipolar cells contain serotonin, tyrosine hydroxylase, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1134-4
更新日期：2005-09-01 00:00:00
abstract：:Bone marrow (BM) from human and rodent species contains a population of multipotential cells referred to as mesenchymal stem cells (MSCs). Currently, MSCs are isolated indirectly by using a culture step and then the generation of fibroblast colony-forming units (CFU-fs). Unprocessed or native BM MSCs have not yet been...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0292-3
更新日期：2007-03-01 00:00:00
