Abstract:
:Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN + erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitochondria, autophagosomes, autophagocytosed mitochondria, autophagocytosed cytoplasm and degraded material inside autophagosomes were calculated per unit volume of cytoplasm. The results demonstrate that (1) the volume density of mitochondria in the cytoplasm decreases by 34% during maturation from (EN-)- to (EN +)-erythroblasts (P less than 0.001) and by 60% during differentiation from (EN +)-erythroblasts to reticulocytes (P less than 0.001), (2) a fivefold increase in the volume density of autophagosomes in the cytoplasm is noted during maturation from (EN-)- to (EN +)-erythroblasts (P less than 0.01), whereas the value of this parameter remains essentially unchanged during the subsequent differentiation to reticulocytes, (3) no mitochondria are found inside autophagosomes of (EN-)-erythroblasts, whereas mitochondria occupy 26% and 35%, respectively, of the autophagosomal volume in (EN +)-erythroblasts and in reticulocytes. Our results show that autophagocytosis of mitochondria starts at the moment of nuclear extrusion and continues in the bone marrow reticulocytes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Heynen MJ,Tricot G,Verwilghen RLdoi
10.1007/BF00214924subject
Has Abstractpub_date
1985-01-01 00:00:00pages
235-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
239pub_type
杂志文章abstract::An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect specie...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050601
更新日期:1996-06-01 00:00:00
abstract::Glaucoma, a disease of the optic nerve and retina, causes blindness in millions of people worldwide. Currently available therapies for this disease only attempt to reduce intraocular pressure, the major risk factor, without addressing the associated optic neuropathy and retinopathy. Development of glaucoma neuroprotec...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1584-z
更新日期:2013-08-01 00:00:00
abstract::Evidence has already been adduced suggesting that an increase in microtubular number occurs in the tractus hypophyseus of rats stressed by the administration of hypertonic saline, and of rats with congenital diabetes insipidus (CDI). Since the tractus hypophyseus in these animals shows high secretory activity, it seem...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219611
更新日期:1976-06-28 00:00:00
abstract::Light and electron microscopic autoradiography has been employed to define the neuroanatomical patterns of uptake and binding of radiolabelled L-dopa in the endocrine hypothalamus of the rat. A dorsomedial continuum of arcuate and periventricular neurons selectively sequester 3HL-dopa 20 min following its intraventric...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233675
更新日期:1978-12-14 00:00:00
abstract::Intracerebroventricular (ICV) injection of melanin-concentrating hormone (MCH) influences feeding behavior in the goldfish and exerts an anorexigenic action in goldfish brain, unlike its orexigenic action in mammals. Despite a growing body of knowledge concerning MCH function in mammals, the role of MCH in appetite ha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0347-5
更新日期:2007-05-01 00:00:00
abstract::By a regular immunoperoxidase method, alpha-1-proteinase inhibitor (Api) was demonstrated in pancreatic islets in individuals with and without genetic deficiency of Api. Subsequently a double immunoperoxidase method, with two different chromogens (diaminobenzidine-brown and 4-chloro-1-naphthol-blue), was applied on th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218078
更新日期:1986-01-01 00:00:00
abstract::The effects of light and dark on photoreceptors of the brackish-water polychaete annelid Nereis limnicola were studied by electron microscopy. Animals dark-adapted for one or two days exhibited well-formed straight microvilli (rhabdomeres) on the sensory cell processes. Continuous illumination of worms for one or two ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225427
更新日期:1985-01-01 00:00:00
abstract::In the present study, we characterized intra-epithelial leukocytes in the digestive tract of chickens during postnatal development. Their phenotype was characterized by monoclonal antibodies in cryostat sections and the numbers of the different cell-types were counted in the epithelium of the esophagus, proventriculus...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318748
更新日期:1993-11-01 00:00:00
abstract::Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217226
更新日期:1984-01-01 00:00:00
abstract::Surface features of the ependymal linings of the fourth ventricle in the fowl were analyzed employing the scanning electron microscope (SEM). On the floor of the median sulcus, each ependymal cell has a solitary cilium, whereas on both sides of the sulcus, cilia are so densely distributed that the details of the under...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233562
更新日期:1979-03-09 00:00:00
abstract::Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the ne...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215442
更新日期:1988-01-01 00:00:00
abstract::Rab11, a small monomeric GTPase associated with recycling endosomes, is a key molecule in the regulation of vesicular trafficking and is involved in the development and differentiation of many Drosophila tissues through interaction with diverse signaling pathways. In this study, we report for the first time that Rab11...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2500-0
更新日期:2017-02-01 00:00:00
abstract::Fixed cerebral vesicles of mouse foetuses were fractured and examined with the scanning electron microscope. This method provides a study of the three dimensional developmental features of the pseudostratified columnar epithelium up to the formation of the early cortex plate. Matrix cells are a cell population of homo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219466
更新日期:1975-11-12 00:00:00
abstract::Transplantation of a small fragment of the ileum beneath the kidney capsule in syngenic mice results in the formation of a cyst lined with proliferating intestinal epithelium. The duration of the cell cycle in the epithelium was determined (using tritiated thymidine and the FLM method) as 14.5 h, as compared with 11.5...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221125
更新日期:1977-02-02 00:00:00
abstract::Regeneration of rat mast cells was studied by TEM from 10 s to 48 h after secretion of histamine induced by compound 48/80. During the first 2 h, small intracellular cavities, formed during compound exocytosis and containing non-membrane-bound remnants of the granules, tended to coalesce, and after 2 h of incubation r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238658
更新日期:1981-01-01 00:00:00
abstract::With the Bodian method stained fibers were observed in the lobules of the rat liver and with the modified Karnovsky and Roots thiocholine method cholinesterase (presumably acetylcholinesterase (AChE))-positive nerve fibers were found in a pattern similar to that of the Bodian-stained fibers. The AChE-positive nerve fi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219403
更新日期:1976-08-20 00:00:00
abstract::Stereological studies showed that treatment of normal adult human adrenocortical cells in primary culture with ACTH or cyclic-AMP for 2 days results in similar increases in the volume of cells, of the mitochondrial and "membrane space" compartments and of the surface area of the smooth endoplasmic reticulum and mitoch...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218169
更新日期:1978-07-05 00:00:00
abstract::A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218082
更新日期:1986-01-01 00:00:00
abstract::Using techniques for enhanced microtubular preservation (albumin pretreatment of Gray) occipital and pyriform cortices of rats were studied by electron microscopy at various stages of development. A close structural relationship was seen between microtubules (mts), focal membrane densities, synaptic vesicles, and pres...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215777
更新日期:1983-01-01 00:00:00
abstract::Cestodes (tapeworms) are a derived, parasitic clade of the phylum Platyhelminthes (flatworms). The cestode body wall represents an adaptation to its endoparasitic lifestyle. The epidermis forms a non-ciliated syncytium, and both muscular and nervous system are reduced. Morphological differences between cestodes and fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0687-8
更新日期:2003-03-01 00:00:00
abstract::Roles of pituitary growth hormone (GH) in female reproduction are well established. Autocrine and/or paracrine actions of GH in the mammalian ovary have additionally been proposed, although whether the ovary is an extra-pituitary site of GH expression in the laying hen is uncertain. This possibility has therefore been...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0595-7
更新日期:2008-05-01 00:00:00
abstract::Calcitonin receptor-immunoreactivity (CTR-ir) was found in enteric neurons of the mouse gastrointestinal tract from embryonic day 13.5 (E13.5) to post-natal day 28 (P28). CTR-ir occurred in cell bodies in ganglia of the myenteric plexus extending from the esophagus to the colon and in nerve cells of the submucosal gan...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-011-1303-6
更新日期:2012-02-01 00:00:00
abstract::In Syrian hamsters (Mesocricetus auratus), oxytocin (OXT) activity within the medial preoptic-anterior hypothalamus (MPOA-AH) and the ventromedial hypothalamus (VMH) plays an important role in the expression of sexual receptivity. Immunocytochemical analysis with OXT-specific antibodies was used to identify the distri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050993
更新日期:1998-02-01 00:00:00
abstract::The distribution of Mg++-ATPase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline pH cytochemical methods at the electron-microscopic level. Reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. Levamisole, ouabain, and va...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218550
更新日期:1986-01-01 00:00:00
abstract::Placenta-derived stem cells (PDSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal and differentiation and their immunomodulatory properties. Although many studies have characterized various PDSCs biologically, the properties of the sel...
journal_title:Cell and tissue research
pub_type: 临床试验,杂志文章
doi:10.1007/s00441-011-1249-8
更新日期:2011-10-01 00:00:00
abstract::Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 µg/ml, for 48 h. The cell s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03405-4
更新日期:2021-01-18 00:00:00
abstract::Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1094-1
更新日期:2011-04-01 00:00:00
abstract::Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220036
更新日期:1988-10-01 00:00:00
abstract::The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1092-x
更新日期:2005-06-01 00:00:00
abstract::Synexin (annexin VII) is a Ca(2+)- and phospholipid-binding protein which has been proposed to play a role in Ca(2+)-dependent membrane fusion processes. Using a monoclonal antibody against synexin, Mab 10E7, and immunogold, we carried out a semiquantitative localization study of synexin in bovine adrenal medullary ch...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319624
更新日期:1992-08-01 00:00:00