On the mechanism of plasma membrane turnover in the salt gland of ducklings. Implications from DNA content, rates of DNA synthesis, and sites of DNA synthesis during the osmotic stressing and destressing cycle.

Abstract:

:This study provides information on the rates of DNA synthesis, sites of DNA synthesis, and DNA content of the avian salt gland during the osmotic stressing (plasma membrane synthesis) and destressing (plasma membrane turnover) cycle, in an effort to better understand the relationship of cell turnover to the initial events in plasma membrane amplification, differentiation, and turnover. The rate of DNA synthesis increases 12--24 h after the onset of osmotic stress, is maximal at about 24 h of osmotic stress, and decreases thereafter in fully stressed and destressed glands. The maximum DNA and protein content, and the maximum protein/DNA ratio are obtained after about 3 days of stress. Autoradiograms show that at 24 hr of stress 70--80% of DNA synthesis occurs in connective tissue cells and 20--30% in parenchymal cells, but by 6 days of stress, synthesis occurs about equally in these cell groups. Because destressing is characterized by a large decrease in plasma membrane and in glandular protein, but by little DNA turnover or loss, the loss of plasma membrane is likely due to some type of cell dedifferentiation rather than cell turnover.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Hossler FE

doi

10.1007/BF00214782

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

531-40

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

226

pub_type

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