Abstract:
:We investigated the actin filament organization and immunolocalization of actin-binding proteins (alpha-actinin and cortactin) in the podocyte foot processes of eight vertebrate species (lamprey, carp, newt, frog, gecko, turtle, quail, and rat). Three types of actin cytoskeleton were found in these foot processes. (1) A cortical actin network with cortactin filling the space between the plasma membrane and the other actin cytoskeletons described below was found in all of the species examined here. The data indicated that the cortical actin network was the minimal essential actin cytoskeleton for the formation and maintenance of the foot processes in vertebrate podocytes. (2) An actin bundle with alpha-actinin existing along the longitudinal axis of foot process above the level of slit diaphragms was only observed in quail and rat. (3) An actin fascicle consisting of much fewer numbers of actin filaments than that of the actin bundle was observed in the species other than quail and rat, but at various frequencies. These findings suggest that the actin bundle is an additional actin cytoskeleton reflecting a functional state peculiar to quail and rat glomeruli. Considering the higher intraglomerular pressure and the extremely thin filtration barrier in birds and mammals, the foot processes probably mainly protect the thinner filtration barrier from the higher internal pressure occurring in quail and rat glomeruli. Therefore, we consider that the actin bundle plays a crucial role in the mechanical protection of the filtration barrier. Moreover, the actin fascicle may be a potential precursor of the actin bundle.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ichimura K,Kurihara H,Sakai Tdoi
10.1007/s00441-007-0440-4subject
Has Abstractpub_date
2007-09-01 00:00:00pages
541-57issue
3eissn
0302-766Xissn
1432-0878journal_volume
329pub_type
杂志文章abstract::Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell population...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0282-5
更新日期:2007-01-01 00:00:00
abstract::The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051160
更新日期:1998-10-01 00:00:00
abstract::The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminifer...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218294
更新日期:1982-01-01 00:00:00
abstract::Despite the common structure of vertebrates, the development of the vertebral column differs widely between teleosts and tetrapods in several respects, including the ossification of the centrum and the function of the notochord. In contrast to tetrapods, vertebral development in teleosts is not fully understood, parti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2404-z
更新日期:2016-10-01 00:00:00
abstract::17beta-estradiol (E2) is important for crustacean ovarian development. This study aims to investigate the distribution and change pattern of E2 in the ovary, hepatopancreas, thoracic ganglion and brain ganglion as well as Vg-mRNA expression level during ovarian development of Chinese mitten crab Eriocheir sinensis. Re...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2834-x
更新日期:2018-08-01 00:00:00
abstract::By a regular immunoperoxidase method, alpha-1-proteinase inhibitor (Api) was demonstrated in pancreatic islets in individuals with and without genetic deficiency of Api. Subsequently a double immunoperoxidase method, with two different chromogens (diaminobenzidine-brown and 4-chloro-1-naphthol-blue), was applied on th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218078
更新日期:1986-01-01 00:00:00
abstract::Since their introduction to biological imaging, quantum dots (QDs) have progressed from a little known, but attractive, technology to one that has gained broad application in many areas of biology. The versatile properties of these fluorescent nanoparticles have allowed investigators to conduct biological studies with...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-2087-2
更新日期:2015-04-01 00:00:00
abstract::Blue-green fluorescent subependymal cells with intraventricular processes were shown by the fluorescent histochemical method to be distributed from the preoptic recess to the infundibular recess of the frog hypothalamus. Electron microscopy revealed at least two types of CSF-contacting subependymal cells, type 1 conta...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221791
更新日期:1977-01-20 00:00:00
abstract::We have analyzed the distribution of neural crest-derived precursors and the expression of catecholaminergic and neuronal markers in developing adrenal tissue of chick embryos. Undifferentiated neural crest cells are found in presumptive adrenal regions from embryonic day 3 (E3) onward. An increasing proportion of cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0996-1
更新日期:2005-01-01 00:00:00
abstract::The fiber arrangement of the retinogeniculate pathways was investigated in the Japanese monkey chiasm by iontophoretical injections into the lateral geniculate nucleus of wheat germ agglutinin conjugated to horseradish peroxidase. The present data demonstrated that the horizontal and dorsoventral axes of the nasal ret...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000265
更新日期:2000-12-01 00:00:00
abstract::In the atherosclerotic plaque, smooth muscle cells (SMC) acquire an inflammatory phenotype. Resistin and fractalkine (CX3CL1) are found in human atheroma and not in normal arteries. CX3CL1 and CX3CR1 are predominately associated with SMC. We have questioned whether resistin has a role in the expression of CX3CL1 and C...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1510-9
更新日期:2013-01-01 00:00:00
abstract::The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1092-x
更新日期:2005-06-01 00:00:00
abstract::The azygos vein of the rat can be divided into three regions: The proximal cardiac region, where the wall is composed of two and sometimes three layers of cardiac muscle and a thin discontinuous layer of smooth muscle cells. Vascular casts of this region demonstrate layers of capillaries closely following the orientat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221867
更新日期:1986-01-01 00:00:00
abstract::The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219936
更新日期:1975-12-18 00:00:00
abstract::The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particul...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213955
更新日期:1986-01-01 00:00:00
abstract::Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224731
更新日期:1989-04-01 00:00:00
abstract::The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237730
更新日期:1979-06-08 00:00:00
abstract::Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209276
更新日期:1978-05-29 00:00:00
abstract::In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in en...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-02998-9
更新日期:2019-06-01 00:00:00
abstract::The membrane systems of cardiac muscle cells of the euphausiacean Meganychtiphanes norvegica are described. Transverse tubules are found both at the Z-band level (TZ-tubules) and at the H-band level (Th-tubules). Within the sarcomere narrow longitudinal tubules branch off from the TZ-tubules. At the H-band level these...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223109
更新日期:1977-07-15 00:00:00
abstract::The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1889-6
更新日期:2014-09-01 00:00:00
abstract::Marked changes in mice pubic symphysis occur by the end of pregnancy. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider both of these cellular behaviors when investigating t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0463-x
更新日期:2007-10-01 00:00:00
abstract::Progenitor stem cells have been identified, isolated and characterized in numerous tissues and organs. However, their therapeutic potential and the use of these stem cells remain elusive except for a few progenitor cells from bone marrow, umbilical cord blood, eyes and dental pulp. The use of bone marrow-derived hemat...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-012-1471-z
更新日期:2012-10-01 00:00:00
abstract::Besides their well-known function in cellular bioenergetics, the role of mitochondria in signaling regulation of cells homeostasis and survival has been uncovered during the past few decades. Possessing an independent genome and a unique genetic code, mitochondria biosynthesize protective stress response factors, the ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03049-z
更新日期:2019-08-01 00:00:00
abstract::Calcitonin gene-related peptide-immunoreactive cells were identified within the epithelium of distal conducting airways in the human fetus and infant. Several peptides and amines, including calcitonin, have been identified previously within a specific population of airway epithelial cells. These cells, referred to as ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218949
更新日期:1987-12-01 00:00:00
abstract::Transgenic rats for the murine Ren-2 gene display high blood pressure, low circulating levels of angiotensin II, and high renin content in the adrenal glands. Moreover, transgenic rats possess an increased aldosterone secretion (maximal from 6 to 18 weeks of age), paralleling the development of hypertension. To invest...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305774
更新日期:1994-10-01 00:00:00
abstract::Peptidyl-glycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is an enzyme that catalyzes conversion of glycine-extended peptides to alpha-amidated bioactive peptides. Two peptides that are processed at their carboxyl-termini by this enzyme are neuropeptide Y and anglerfish peptide Y, both of which possess a C-te...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00304521
更新日期:1995-04-01 00:00:00
abstract::New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2798-x
更新日期:2018-04-01 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217887
更新日期:1983-01-01 00:00:00