Abstract:
:Mu bacteriophage inserts its DNA into the genome of host bacteria and is used as a model for DNA transposition events in other systems. The eukaryotic Ku protein has key roles in DNA repair and in certain transposition events. Here we show that the Gam protein of phage Mu is conserved in bacteria, has sequence homology with both subunits of Ku, and has the potential to adopt a similar architecture to the core DNA-binding region of Ku. Through biochemical studies, we demonstrate that Gam and the related protein of Haemophilus influenzae display DNA binding characteristics remarkably similar to those of human Ku. In addition, we show that Gam can interfere with Ty1 retrotransposition in Saccharomyces cerevisiae. These data reveal structural and functional parallels between bacteriophage Gam and eukaryotic Ku and suggest that their functions have been evolutionarily conserved.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
d'Adda di Fagagna F,Weller GR,Doherty AJ,Jackson SPdoi
10.1038/sj.embor.embor709keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
47-52issue
1eissn
1469-221Xissn
1469-3178pii
embor709journal_volume
4pub_type
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