Abstract:
:Subcellular organization is yielding to large-scale analysis. Researchers are now applying robust mass-spectrometry-based proteomics methods to obtain an inventory of biochemically isolated organelles that contain hundreds of proteins. High-resolution methods allow accurate protein identification, and novel algorithms can distinguish genuine from co-purifying components. Organellar proteomes have been analysed by bioinformatic methods and integrated with other large-scale data sets. The dynamics of organelles can also be studied by quantitative proteomics, which offers powerful methods that are complementary to fluorescence-based microscopy. Here, we review the emerging trends in this rapidly expanding area and discuss the role of organellar proteomics in the context of functional genomics and systems biology.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Andersen JS,Mann Mdoi
10.1038/sj.embor.7400780subject
Has Abstractpub_date
2006-09-01 00:00:00pages
874-9issue
9eissn
1469-221Xissn
1469-3178pii
7400780journal_volume
7pub_type
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