Abstract:
:While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the identification of novel selenoprotein genes. Here, we describe a comparative genomics approach that relies on the genome-wide prediction of genes with in-frame TGA codons, and the subsequent comparison of predictions from different genomes, wherein conservation in regions flanking the TGA codon suggests selenocysteine coding function. Application of this method to human and fugu genomes identified a novel selenoprotein family, named SelU, in the puffer fish. The selenocysteine-containing form also occurred in other fish, chicken, sea urchin, green algae and diatoms. In contrast, mammals, worms and land plants contained cysteine homologues. We demonstrated selenium incorporation into chicken SelU and characterized the SelU expression pattern in zebrafish embryos. Our data indicate a scattered evolutionary distribution of selenoproteins in eukaryotes, and suggest that, contrary to the picture emerging from data available so far, other taxa-specific selenoproteins probably exist.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Castellano S,Novoselov SV,Kryukov GV,Lescure A,Blanco E,Krol A,Gladyshev VN,Guigó Rdoi
10.1038/sj.embor.7400036keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
71-7issue
1eissn
1469-221Xissn
1469-3178pii
7400036journal_volume
5pub_type
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