Abstract:
:The impact of the mitochondrial permeability transition (MPT) on cellular physiology is well characterized. In contrast, the composition and mode of action of the permeability transition pore complex (PTPC), the supramolecular entity that initiates MPT, remain to be elucidated. Specifically, the precise contribution of the mitochondrial F1FO ATP synthase (or subunits thereof) to MPT is a matter of debate. We demonstrate that F1FO ATP synthase dimers dissociate as the PTPC opens upon MPT induction. Stabilizing F1FO ATP synthase dimers by genetic approaches inhibits PTPC opening and MPT Specific mutations in the F1FO ATP synthase c subunit that alter C-ring conformation sensitize cells to MPT induction, which can be reverted by stabilizing F1FO ATP synthase dimers. Destabilizing F1FO ATP synthase dimers fails to trigger PTPC opening in the presence of mutants of the c subunit that inhibit MPT The current study does not provide direct evidence that the C-ring is the long-sought pore-forming subunit of the PTPC, but reveals that PTPC opening requires the dissociation of F1FO ATP synthase dimers and involves the C-ring.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Bonora M,Morganti C,Morciano G,Pedriali G,Lebiedzinska-Arciszewska M,Aquila G,Giorgi C,Rizzo P,Campo G,Ferrari R,Kroemer G,Wieckowski MR,Galluzzi L,Pinton Pdoi
10.15252/embr.201643602subject
Has Abstractpub_date
2017-07-01 00:00:00pages
1077-1089issue
7eissn
1469-221Xissn
1469-3178pii
embr.201643602journal_volume
18pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::An effective immune response requires the engagement of host receptors by pathogen-derived molecules and the stimulation of an appropriate cellular response. Therefore, a crucial factor in our ability to control an infection is the accessibility of our immune cells to the foreign material. Exosomes-which are extracell...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201439363
更新日期:2015-01-01 00:00:00
abstract::The crystal structure of the ligand-binding domain of RARbeta, a suspect tumour suppressor, reveals important features that distinguish it from the two other RAR isotypes. The most striking difference is an extra cavity allowing RARbeta to bind more bulky agonists. Accordingly, we identified a ligand that shows RARbet...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400235
更新日期:2004-09-01 00:00:00
abstract::The aggregation of the two yeast proteins Sup35p and Ure2p is widely accepted as a model for explaining the prion propagation of the phenotypes [PSI+] and [URE3], respectively. Here, we demonstrate that the propagation of [URE3] cannot simply be the consequence of generating large aggregates of Ure2p, because such agg...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf011
更新日期:2002-01-01 00:00:00
abstract::RNA modifications have recently emerged as an important layer of gene regulation. N6-methyladenosine (m6 A) is the most prominent modification on eukaryotic messenger RNA and has also been found on noncoding RNA, including ribosomal and small nuclear RNA. Recently, several m6 A methyltransferases were identified, unco...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949443
更新日期:2020-07-03 00:00:00
abstract::The second edition of the EMBO Spatial meeting took place in May 2011 in Engelberg, Switzerland. Although the work presented at the meeting covered a challengingly broad range of topics, it accomplished the rare goal of promoting interactions between cell biologists, neuroscientists, systems biologists and mathematici...
journal_title:EMBO reports
pub_type:
doi:10.1038/embor.2011.169
更新日期:2011-09-01 00:00:00
abstract::The gastrointestinal tract undergoes homeostatic self-renewal to replace aged and damaged epithelial cells. This process, sustained by intestinal stem cells (ISCs), can operate accurately for many years but gradually declines with age. Although stem cell aging has been intensively explored, the mechanisms remain poorl...
journal_title:EMBO reports
pub_type: 评论,新闻
doi:10.15252/embr.202051175
更新日期:2020-08-05 00:00:00
abstract::Microglia are specialized parenchymal-resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846171
更新日期:2018-11-01 00:00:00
abstract::Histone deacetylases (HDACs)-an enzyme family that deacetylates histones and non-histone proteins-are implicated in human diseases such as cancer, and the first-generation of HDAC inhibitors are now in clinical trials. Here, we report the 2.0 A resolution crystal structure of a catalytically inactive HDAC8 active-site...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401047
更新日期:2007-09-01 00:00:00
abstract::β-arrestin 1 and 2 (also known as arrestin 2 and 3) are homologous adaptor proteins that regulate seven-transmembrane receptor trafficking and signalling. Other proteins with predicted 'arrestin-like' structural domains but lacking sequence homology have been indicated to function like β-arrestin in receptor regulatio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.187
更新日期:2013-02-01 00:00:00
abstract::The natural progressive dysfunction of most living organisms-ageing-has captured the attention of several studies, with the intention to develop rejuvenation strategies. Evidence is emerging of a positive correlation between natural ageing and chromosomal instability (CIN). In this issue of EMBO Reports, Barroso-Vilar...
journal_title:EMBO reports
pub_type: 评论,新闻
doi:10.15252/embr.202050322
更新日期:2020-05-06 00:00:00
abstract::Caspase-3 is a crucial component of the apoptotic machinery in many cell types. Here, we report the timescale of caspase-3 activation in single living cells undergoing apoptosis. This was achieved by measuring the extent of fluorescence resonance energy transfer within a recombinant substrate containing cyan fluoresce...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd050
更新日期:2000-09-01 00:00:00
abstract::The components and pathways that regulate and execute developmental cell death programmes in plants remain largely unknown. We have found that the PROMOTION OF CELL SURVIVAL 1 (PCS1) gene in Arabidopsis, which encodes an aspartic protease, has an important role in determining the fate of cells in embryonic development...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400357
更新日期:2005-03-01 00:00:00
abstract::Reading the academic literature is an essential part of a scientist's job. David Smith argues that reading fiction can be even more important for creativity and productivity than papers and reviews. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202052206
更新日期:2021-01-04 00:00:00
abstract::Synaptic mitochondria are particularly vulnerable to physiological insults, and defects in synaptic mitochondria are linked to early pathophysiology of Alzheimer's disease (AD). Mitophagy, a cargo-specific autophagy for elimination of dysfunctional mitochondria, constitutes a key quality control mechanism. However, ho...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949801
更新日期:2020-09-03 00:00:00
abstract::WHITE COLLAR-1 (WC-1) is the limiting component of the White Collar Complex (WCC) controlling expression of the Neurospora circadian clock protein Frequency (FRQ). Accumulation of WC-1 is supported by FRQ on a post-transcriptional level. Here, we show that transcription of wc-1 is organized in a complex way. Three pro...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400595
更新日期:2006-02-01 00:00:00
abstract::Amino acids are essential for cellular metabolism, and it is important to understand how nutrient supply is coordinated with changing energy requirements during embryogenesis. Here, we show that the amino acid transporter Slc7a5/Lat1 is highly expressed in tissues undergoing morphogenesis and that Slc7a5-null mouse em...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948469
更新日期:2020-01-07 00:00:00
abstract::The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (DeltaN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we s...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400623
更新日期:2006-04-01 00:00:00
abstract::Given that ligand binding is essential for the rapid internalization of epidermal growth factor receptor (EGFR), the events induced by ligand binding probably contribute to the regulation of EGFR internalization. These events include receptor dimerization, activation of intrinsic tyrosine kinase activity and autophosp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400491
更新日期:2005-10-01 00:00:00
abstract::Accumulating evidence highlights the role of histone acetyltransferase GCN5 in the regulation of cell metabolism in metazoans. Here, we report that GCN5 is a negative regulator of autophagy, a lysosome-dependent catabolic mechanism. In animal cells and Drosophila, GCN5 inhibits the biogenesis of autophagosomes and lys...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948335
更新日期:2020-01-07 00:00:00
abstract::The Wnt signaling pathway can be grouped into two classes, the β-catenin-dependent and β-catenin-independent pathways. Wnt5a signaling through a β-catenin-independent pathway promotes microtubule (MT) remodeling during cell-substrate adhesion, cell migration, and planar cell polarity formation. Although Wnt5a signalin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201745471
更新日期:2018-07-01 00:00:00
abstract::The Polycomb group (PcG) of proteins conveys epigenetic inheritance of repressed transcriptional states. In Drosophila, the Polycomb repressive complex 1 (PRC1) maintains the silent state by inhibiting the transcription machinery and chromatin remodelling at core promoters. Using immunoprecipitation of in vivo formald...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400260
更新日期:2004-10-01 00:00:00
abstract::Sonic hedgehog (Shh), both as a mitogen and as a morphogen, plays an important role in cell proliferation and differentiation during early development. Here, we show that Shh inhibits glutamate transporter activities in neurons, rapidly enhances extracellular glutamate levels, and affects the development of epilepsy. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201541569
更新日期:2016-05-01 00:00:00
abstract::KCC2, encoded in humans by the SLC12A5 gene, is a multifunctional neuron-specific protein initially identified as the chloride (Cl- ) extruder critical for hyperpolarizing GABAA receptor currents. Independently of its canonical function as a K-Cl cotransporter, KCC2 regulates the actin cytoskeleton via molecular inter...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948880
更新日期:2020-04-03 00:00:00
abstract::Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Her...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400775
更新日期:2006-10-01 00:00:00
abstract::The cytoplasm is the main place for protein translation from where nascent proteins are transported to their working areas, including the inside, outside, and membrane of the cell. The majority of newly synthesized membrane proteins is co-translationally inserted into the membrane by the evolutionary conserved Sec tra...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.15252/embr.201949034
更新日期:2019-10-04 00:00:00
abstract::Brinker is a nuclear protein that antagonizes Dpp signalling in Drosophila. Its expression is negatively regulated by Dpp. Here, we show that Brinker represses Ultrabithorax (Ubx) in the embryonic midgut, a HOX gene that activates, and responds to, the localized expression of Dpp during endoderm induction. We find tha...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve068
更新日期:2001-04-01 00:00:00
abstract::Hydrogen sulfide (H2 S), an endogenous signaling gaseous molecule, is involved in various physiological activities, including vessel relaxation, regulation of cellular bioenergetics, inflammation, and angiogenesis. By using xenograft orthotopic implantation of prostate cancer PC3 cells and subsequently comparing bone ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201845986
更新日期:2019-10-04 00:00:00
abstract::Ubiquitin is a post-translational modifier with proteolytic and non-proteolytic roles in many biological processes. At mitochondria, it performs regulatory homeostatic functions and contributes to mitochondrial quality control. Ubiquitin is essential for mitochondrial fusion, regulates mitochondria-ER contacts, and pa...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201338225
更新日期:2014-03-01 00:00:00
abstract::When our time comes to die most people would probably opt for a quick, peaceful and painless exit. But the manner and timing are rarely under our direct control. Hence the Ars moriendi, literally, "The Art of Dying", two texts written in Latin around the 15th century that offered advice on how to die well according to...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201337970
更新日期:2014-02-01 00:00:00
abstract::Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes includin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642194
更新日期:2016-10-01 00:00:00