RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.

abstract：:Centromeric constitutive heterochromatin is marked by DNA methylation and dimethylated histone H3 Lys 9 (H3K9me2) in Arabidopsis. RNA-directed DNA methylation (RdDM) is a process that uses 24-nucleotide (nt) small interfering RNAs (siRNAs) to induce de novo methylation to its homologous DNA sequences. Despite the pres...

journal_title：EMBO reports

authors： Schoft VK,Chumak N,Mosiolek M,Slusarz L,Komnenovic V,Brownfield L,Twell D,Kakutani T,Tamaru H

更新日期：2009-09-01 00:00:00

abstract：:Labmates are colleagues, friends, comforters…and competitors. ...

journal_title：EMBO reports

authors： Morriswood B,Hoeller O

更新日期：2019-07-01 00:00:00

abstract：:STK38 (also known as NDR1) is a Hippo pathway serine/threonine protein kinase with multifarious functions in normal and cancer cells. Using a context-dependent proximity-labeling assay, we identify more than 250 partners of STK38 and find that STK38 modulates its partnership depending on the cellular context by increa...

journal_title：EMBO reports

authors： Martin AP,Jacquemyn M,Lipecka J,Chhuon C,Aushev VN,Meunier B,Singh MK,Carpi N,Piel M,Codogno P,Hergovich A,Parrini MC,Zalcman G,Guerrera IC,Daelemans D,Camonis JH

更新日期：2019-11-05 00:00:00

abstract：:The adenovirus early gene product Gam1 is crucial for virus replication and induces certain cellular genes by inactivating histone deacetylase 1 (HDAC1). We demonstrate that Gam1 (i) destroys promyelocitic leukemia nuclear bodies, (ii) delocalizes SUMO-1 into the cytoplasm and (iii) influences the SUMO-1 pathway. In a...

journal_title：EMBO reports

authors： Colombo R,Boggio R,Seiser C,Draetta GF,Chiocca S

更新日期：2002-11-01 00:00:00

abstract：:The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that th...

journal_title：EMBO reports

authors： Bomar MG,Pai MT,Tzeng SR,Li SS,Zhou P

更新日期：2007-03-01 00:00:00

abstract：:The second edition of the EMBO Spatial meeting took place in May 2011 in Engelberg, Switzerland. Although the work presented at the meeting covered a challengingly broad range of topics, it accomplished the rare goal of promoting interactions between cell biologists, neuroscientists, systems biologists and mathematici...

journal_title：EMBO reports

authors： Tzlil S,Kuruvilla R

更新日期：2011-09-01 00:00:00

abstract：:In Drosophila, a cascade of maternal, gap, pair-rule and segment polarity genes subdivides the antero/posterior axis of the embryo into repeating segmental stripes. This review summarizes what happens next, i.e. how an intrasegmental pattern is generated and controls the differentiation of specific cell types in the e...

journal_title：EMBO reports

authors： Sanson B

更新日期：2001-12-01 00:00:00

abstract：:The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...

journal_title：EMBO reports

authors： Patel JR,Jain A,Chou YY,Baum A,Ha T,García-Sastre A

更新日期：2013-09-01 00:00:00

abstract：:Embryonic stem cell (ESC) cultures display a heterogeneous gene expression profile, ranging from a pristine naïve pluripotent state to a primed epiblast state. Addition of inhibitors of GSK3β and MEK (so-called 2i conditions) pushes ESC cultures toward a more homogeneous naïve pluripotent state, but the molecular unde...

journal_title：EMBO reports

authors： Welling M,Chen HH,Muñoz J,Musheev MU,Kester L,Junker JP,Mischerikow N,Arbab M,Kuijk E,Silberstein L,Kharchenko PV,Geens M,Niehrs C,van de Velde H,van Oudenaarden A,Heck AJ,Geijsen N

更新日期：2015-07-01 00:00:00

abstract：:The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, and membrane or organelle dynamics. However, its filamentous properties and functions inside the mammalian cell nucleus are less well explored. We previously described transient actin assembly at...

journal_title：EMBO reports

authors： Krippner S,Winkelmeier J,Knerr J,Brandt DT,Virant D,Schwan C,Endesfelder U,Grosse R

更新日期：2020-11-05 00:00:00

abstract：:Global climate change and the increasing human population require crop varieties with higher yield and draught resistance. But meeting both goals is not an easy task for breeders and plant science. ...

journal_title：EMBO reports

authors： Moshelion M

更新日期：2020-11-29 00:00:00

abstract：:Anopheles gambiae, the major vector of human malaria parasite, is an important insect model to study vector-parasite interactions. Here, we developed a simple in vivo double-stranded RNA (dsRNA) knockout approach to determine the function of the mosquito antimicrobial peptide gene Defensin. We injected dsRNA into adul...

journal_title：EMBO reports

authors： Blandin S,Moita LF,Köcher T,Wilm M,Kafatos FC,Levashina EA

更新日期：2002-09-01 00:00:00

abstract：:X inactivation is the mechanism by which mammals adjust the genetic imbalance that arises from the different numbers of gene-rich X-chromosomes between the sexes. The dosage difference between XX females and XY males is functionally equalized by silencing one of the two X chromosomes in females. This dosage-compensati...

journal_title：EMBO reports

authors： Ng K,Pullirsch D,Leeb M,Wutz A

更新日期：2007-01-01 00:00:00

abstract：:The Lgr5 receptor is a marker of intestinal stem cells (ISCs) that regulates Wnt/b-catenin signaling. In this study, phenotype analysis of knockin/knockout Lgr5-eGFP-IRES-Cre and Lgr5-DTReGFP embryos reveals that Lgr5 deficiency during Wnt-mediated cytodifferentiation results in amplification of ISCs and early differe...

journal_title：EMBO reports

authors： Fernandez Vallone V,Leprovots M,Ribatallada-Soriano D,Gerbier R,Lefort A,Libert F,Vassart G,Garcia MI

更新日期：2020-07-03 00:00:00

abstract：:The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the ce...

journal_title：EMBO reports

authors： Okada M,Takezawa S,Mezaki Y,Yamaoka I,Takada I,Kitagawa H,Kato S

更新日期：2008-06-01 00:00:00

abstract：:Immune signaling networks must be tunable to alleviate fitness costs associated with immunity and, at the same time, robust against pathogen interferences. How these properties mechanistically emerge in plant immune signaling networks is poorly understood. Here, we discovered a molecular mechanism by which the model p...

journal_title：EMBO reports

authors： Mine A,Nobori T,Salazar-Rondon MC,Winkelmüller TM,Anver S,Becker D,Tsuda K

更新日期：2017-03-01 00:00:00

abstract：:Post-translational protein arginylation is essential for cardiovascular development and angiogenesis in mice and is mediated by arginyl-transfer RNA-protein transferases Ate1-a functionally conserved but poorly understood class of enzymes. Here, we used sequence analysis to detect the evolutionary relationship between...

journal_title：EMBO reports

authors： Rai R,Mushegian A,Makarova K,Kashina A

更新日期：2006-08-01 00:00:00

abstract：:Expression of the tight junction protein junctional adhesion molecule-A (JAM-A) has been linked to proliferation and tumour progression. However, a direct role for JAM-A in regulating proliferative processes has not been shown. By using complementary in vivo and in vitro approaches, we demonstrate that JAM-A restricts...

journal_title：EMBO reports

authors： Nava P,Capaldo CT,Koch S,Kolegraff K,Rankin CR,Farkas AE,Feasel ME,Li L,Addis C,Parkos CA,Nusrat A

更新日期：2011-04-01 00:00:00

abstract：:Import of peroxisomal matrix proteins is essential for peroxisome biogenesis. Genetic and biochemical studies using a variety of different model systems have led to the discovery of 23 PEX genes required for this process. Although it is generally believed that, in contrast to mitochondria and chloroplasts, translocati...

journal_title：EMBO reports

authors： Einwächter H,Sowinski S,Kunau WH,Schliebs W

更新日期：2001-11-01 00:00:00

abstract：:Trypanosomes use RNA editing to produce most functional mitochondrial messenger RNA. Precise insertion and deletion of hundreds of uridines is necessary to make full-length cytochrome c oxidase III (COXIII) mRNA. We show that COXIII mRNA can be alternatively edited by a mechanism using an alternative guide RNA to make...

journal_title：EMBO reports

authors： Ochsenreiter T,Hajduk SL

更新日期：2006-11-01 00:00:00

abstract：:Most solid human tumours are aneuploid, that is, they contain an abnormal number of chromosomes. Paradoxically, however, aneuploidy has been reported to induce a stress response that suppresses cellular proliferation in vitro. Here, we review the progress in our understanding of the causes and effects of aneuploidy in...

journal_title：EMBO reports

authors： Holland AJ,Cleveland DW

更新日期：2012-06-01 00:00:00

abstract：:The COP9 signalosome (CSN) is a highly conserved protein complex that was originally described as a repressor of light-dependent growth and transcription in Arabidopsis. The most studied CSN function is the regulation of protein degradation, which occurs primarily through the removal of the ubiquitin-like modifier Ned...

journal_title：EMBO reports

authors： Chamovitz DA

更新日期：2009-04-01 00:00:00

abstract：:Histone demethylases-both lysine-specific demethylase 1 (LSD1) and Jumonji-C (JmjC) domain-containing proteins-are broadly implicated in the regulation of chromatin-dependent processes. In Arabidopsis thaliana, histone marks directly affect DNA methylation, and mutations in LSD1 homologues show reduced DNA methylation...

journal_title：EMBO reports

authors： Deleris A,Greenberg MV,Ausin I,Law RW,Moissiard G,Schubert D,Jacobsen SE

更新日期：2010-12-01 00:00:00

abstract：:The eukaryotic GINS complex has an essential role in the initiation and elongation phases of genome duplication. It is composed of four paralogous subunits--Sld5, Psf1, Psf2 and Psf3--which are ubiquitous and evolutionarily conserved in eukaryotic organisms. Here, we report the biochemical characterization of the huma...

journal_title：EMBO reports

authors： De Falco M,Ferrari E,De Felice M,Rossi M,Hübscher U,Pisani FM

更新日期：2007-01-01 00:00:00

abstract：:Loss of primary cilia is a key feature of von Hippel-Lindau tumor suppressor (VHL)-associated pathology. Although VHL-deficiency predisposes cells to precipitous cilia disassembly in response to growth factor cues, it does not affect ciliogenesis. Here, using a siRNA-based screen to find genes that are essential for c...

journal_title：EMBO reports

authors： Troilo A,Alexander I,Muehl S,Jaramillo D,Knobeloch KP,Krek W

更新日期：2014-01-01 00:00:00

abstract：:The bacterium Bacillus subtilis produces the DNA integrity scanning protein (DisA), a checkpoint protein that delays sporulation in response to DNA damage. DisA scans the chromosome and pauses at sites of DNA lesions. Structural analysis showed that DisA synthesizes the small molecule cyclic diadenosine monophosphate ...

journal_title：EMBO reports

authors： Oppenheimer-Shaanan Y,Wexselblatt E,Katzhendler J,Yavin E,Ben-Yehuda S

更新日期：2011-06-01 00:00:00

abstract：:Ligands for nuclear receptors facilitate the exchange of co-repressors for coactivators, leading to chromatin modifications that favour the activation of gene transcription. Here, we show that the repressed state of an endogenous retinoic acid-regulated gene is quickly re-established after ligand removal. As expected,...

journal_title：EMBO reports

authors： Hartman HB,Yu J,Alenghat T,Ishizuka T,Lazar MA

更新日期：2005-05-01 00:00:00

abstract：:Sister chromatid cohesion is the basis for the recognition of chromosomal DNA replication products for their bipolar segregation in mitosis. Fundamental to sister chromatid cohesion is the ring-shaped cohesin complex, which is loaded onto chromosomes long before the initiation of DNA replication and is thought to hold...

journal_title：EMBO reports

authors： Uhlmann F

更新日期：2009-10-01 00:00:00

abstract：:Caspase-3 is a crucial component of the apoptotic machinery in many cell types. Here, we report the timescale of caspase-3 activation in single living cells undergoing apoptosis. This was achieved by measuring the extent of fluorescence resonance energy transfer within a recombinant substrate containing cyan fluoresce...

journal_title：EMBO reports

authors： Tyas L,Brophy VA,Pope A,Rivett AJ,Tavaré JM

更新日期：2000-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) regulate target mRNAs by silencing them. Reciprocally, however, target mRNAs can also modulate miRNA stability. Here, we uncover a remarkable efficacy of target RNA-directed miRNA degradation (TDMD) in rodent primary neurons. Coincident with degradation, and while still bound to Argonaute, targeted ...

journal_title：EMBO reports

authors： de la Mata M,Gaidatzis D,Vitanescu M,Stadler MB,Wentzel C,Scheiffele P,Filipowicz W,Großhans H

更新日期：2015-04-01 00:00:00