Abstract:
:Biasing the sex ratio of populations of different organisms, including plants, insects, crustacean, and fish, has been demonstrated by genetic and non-genetic approaches. However, biasing the sex ratio of mammalian populations has not been demonstrated genetically. Here, we provide a first proof of concept for such a genetic system in mammals by crossing two genetically engineered mouse lines. The maternal line encodes a functional Cas9 protein on an autosomal chromosome, whereas the paternal line encodes guide RNAs on the Y chromosome targeting vital mouse genes. After fertilization, the presence of both the Y-encoded guide RNAs from the paternal sperm and the Cas9 protein from the maternal egg targets the vital genes in males. We show that these genes are specifically targeted in males and that this breeding consequently self-destructs solely males. Our results pave the way for a genetic system that allows biased sex production of livestock.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Yosef I,Edry-Botzer L,Globus R,Shlomovitz I,Munitz A,Gerlic M,Qimron Udoi
10.15252/embr.201948269subject
Has Abstractpub_date
2019-08-01 00:00:00pages
e48269issue
8eissn
1469-221Xissn
1469-3178journal_volume
20pub_type
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