A genetic system for biasing the sex ratio in mice.

Abstract:

:Biasing the sex ratio of populations of different organisms, including plants, insects, crustacean, and fish, has been demonstrated by genetic and non-genetic approaches. However, biasing the sex ratio of mammalian populations has not been demonstrated genetically. Here, we provide a first proof of concept for such a genetic system in mammals by crossing two genetically engineered mouse lines. The maternal line encodes a functional Cas9 protein on an autosomal chromosome, whereas the paternal line encodes guide RNAs on the Y chromosome targeting vital mouse genes. After fertilization, the presence of both the Y-encoded guide RNAs from the paternal sperm and the Cas9 protein from the maternal egg targets the vital genes in males. We show that these genes are specifically targeted in males and that this breeding consequently self-destructs solely males. Our results pave the way for a genetic system that allows biased sex production of livestock.

journal_name

EMBO Rep

journal_title

EMBO reports

authors

Yosef I,Edry-Botzer L,Globus R,Shlomovitz I,Munitz A,Gerlic M,Qimron U

doi

10.15252/embr.201948269

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

e48269

issue

8

eissn

1469-221X

issn

1469-3178

journal_volume

20

pub_type

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