Abstract:
:Processive motor proteins are stochastic steppers that perform actual mechanical steps for only a minor fraction of the time they are bound to the filament track. Motors usually work in teams and therefore the question arises whether the stochasticity of stepping can cause mutual interference when motors are mechanically coupled. We used biocompatible surfaces to immobilize processive kinesin-1 motors at controlled surface densities in a mechanically well-defined way. This helped us to study quantitatively how mechanical coupling between motors affects the efficiency of collective microtubule transport. We found that kinesin-1 constructs that lack most of the non-motor sequence slow each other down when collectively transporting a microtubule, depending on the number of interacting motors. This negative interference observed for a motor ensemble can be explained quantitatively by a mathematical model using the known physical properties of individual molecules of kinesin-1. The non-motor extension of kinesin-1 reduces this mutual interference, indicating that loose mechanical coupling between motors is required for efficient transport by ensembles of processive motors.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Bieling P,Telley IA,Piehler J,Surrey Tdoi
10.1038/embor.2008.169subject
Has Abstractpub_date
2008-11-01 00:00:00pages
1121-7issue
11eissn
1469-221Xissn
1469-3178pii
embor2008169journal_volume
9pub_type
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