Abstract:
:Given that ligand binding is essential for the rapid internalization of epidermal growth factor receptor (EGFR), the events induced by ligand binding probably contribute to the regulation of EGFR internalization. These events include receptor dimerization, activation of intrinsic tyrosine kinase activity and autophosphorylation. Whereas the initial results are controversial regarding the role of EGFR kinase activity in EGFR internalization, more recent data suggest that EGFR kinase activation is essential for EGFR internalization. However, we have shown here that inhibition of EGFR kinase activation by mutation or by chemical inhibitors did not block EGF-induced EGFR internalization. Instead, proper EGFR dimerization is necessary and sufficient to stimulate EGFR internalization. We conclude that EGFR internalization is controlled by EGFR dimerization, rather than EGFR kinase activation. Our results also define a new role for EGFR dimerization: by itself it can drive EGFR internalization, independent of its role in the activation of EGFR kinase.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Wang Q,Villeneuve G,Wang Zdoi
10.1038/sj.embor.7400491keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
942-8issue
10eissn
1469-221Xissn
1469-3178pii
7400491journal_volume
6pub_type
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