Abstract:
:The active and inactive X chromosomes have distinct epigenetic marks in somatic nuclei, which undergo reprogramming after transplantation into oocytes. We show that, despite the disappearance of Xist RNA coating in 30 min, the epigenetic memory of the inactive X persists with the precocious appearance of histone H3 trimethylation of lysine 27 (H3-3meK27), without the expected colocalization with Eed/Ezh2. Subsequently, Xist re-appears on the original inactive X, and the silent Xist on the active X undergoes re-activation, resulting in unusual biallelic Xist RNA domains. Despite this abnormal Xist expression pattern, colocalization of H3-3meK27 and Eed is thereafter confined to a single Xist domain, which is presumably on the original inactive X. These epigenetic events differ markedly from the kinetics of preferential paternal X inactivation in normal embryos. All the epigenetic marks on the X are apparently erased in the epiblast, suggesting that the oocyte and epiblast may have distinct properties for stepwise programming of the genome.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Bao S,Miyoshi N,Okamoto I,Jenuwein T,Heard E,Azim Surani Mdoi
10.1038/sj.embor.7400461keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
748-54issue
8eissn
1469-221Xissn
1469-3178pii
7400461journal_volume
6pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-grad...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor799
更新日期:2003-04-01 00:00:00
abstract::Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes includin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642194
更新日期:2016-10-01 00:00:00
abstract::Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, eac...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.151
更新日期:2013-11-01 00:00:00
abstract::We have designed a doxycycline-regulated form of the H1 promoter of RNA polymerase III that allows the inducible knockdown of gene expression by small interfering RNAs (siRNAs). As a proof-of-principle, we have targeted beta-catenin in colorectal cancer (CRC) cells. T-cell factor (TCF) target-gene expression is induce...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor865
更新日期:2003-06-01 00:00:00
abstract::In yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA--U3, U14 and snR30--whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the pre-ribosome association to all 75 yeast snoRNAs in strains de...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.184
更新日期:2008-12-01 00:00:00
abstract::BAT-controlled thermogenic activity is thought to be required for its capacity to prevent the development of insulin resistance. This hypothesis predicts that mediators of thermogenesis may help prevent diet-induced insulin resistance. We report that the mitochondrial fusion protein Mitofusin 2 (Mfn2) in BAT is essent...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643827
更新日期:2017-07-01 00:00:00
abstract::Cap-binding proteins of the elF4E family are generally involved in mediating ribosome recruitment to capped mRNA via an interaction with the initiation factor elF4G. However, Schizosaccharomyces pombe has two elF4E isoforms, one of which (elF4E2, encoded by tif452) has a relatively low affinity for elF4G. We show that...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400088
更新日期:2004-03-01 00:00:00
abstract::Centromeric constitutive heterochromatin is marked by DNA methylation and dimethylated histone H3 Lys 9 (H3K9me2) in Arabidopsis. RNA-directed DNA methylation (RdDM) is a process that uses 24-nucleotide (nt) small interfering RNAs (siRNAs) to induce de novo methylation to its homologous DNA sequences. Despite the pres...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.152
更新日期:2009-09-01 00:00:00
abstract::Signal peptide peptidase (SPP) and the four homologous SPP-like (SPPL) proteases constitute a family of intramembrane aspartyl proteases with selectivity for type II-oriented transmembrane segments. Here, we analyse the physiological function of the orphan protease SPPL2c, previously considered to represent a non-expr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846449
更新日期:2019-03-01 00:00:00
abstract::Sepsis, or blood poisoning, is a savage response of the body to infection. It can lead to organ failure, blood pressure decline, heart failure, and coma. Between 20 and 30 million people suffer from sepsis each year, leading to 8 million deaths. Although certain people are more at risk than others (young children, eld...
journal_title:EMBO reports
pub_type: 评论,新闻
doi:10.15252/embr.201949193
更新日期:2020-01-07 00:00:00
abstract::Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic expression of E2F3 results in...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400452
更新日期:2005-07-01 00:00:00
abstract::The Vav family of proteins are guanine nucleotide exchange factors (GEFs) for the Rho family of GTPases, which regulate various cellular functions, including T-cell activation. They contain a catalytic Dbl homology (DH) domain that is invariably followed by a pleckstrin homology (PH) domain, which is often required fo...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.80
更新日期:2008-07-01 00:00:00
abstract::N6-methyladenosine (m6A) is a highly dynamic RNA modification that has recently emerged as a key regulator of gene expression. While many m6A modifications are installed by the METTL3-METTL14 complex, others appear to be introduced independently, implying that additional human m6A methyltransferases remain to be ident...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744940
更新日期:2017-11-01 00:00:00
abstract::The highly homologous Cl(-) channels CLC-Ka and CLC-Kb are important for water and salt conservation in the kidney and for the production of endolymph in the inner ear. Mutations in CLC-Kb lead to Bartter's syndrome and mutations in the small CLC-K subunit barttin lead to Bartter's syndrome and deafness. Here we show ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400169
更新日期:2004-06-01 00:00:00
abstract::When our time comes to die most people would probably opt for a quick, peaceful and painless exit. But the manner and timing are rarely under our direct control. Hence the Ars moriendi, literally, "The Art of Dying", two texts written in Latin around the 15th century that offered advice on how to die well according to...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201337970
更新日期:2014-02-01 00:00:00
abstract::Scrapie, bovine spongiform encephalopathy and chronic wasting disease are orally communicable, transmissible spongiform encephalopathies (TSEs). As zoonotic transmissions of TSE agents may pose a risk to human health, the identification of reservoirs for infectivity in animal tissues and their exclusion from human con...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor827
更新日期:2003-05-01 00:00:00
abstract::In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337898
更新日期:2014-04-01 00:00:00
abstract::The tumour suppressor ARF (alternative reading frame), which is mutated or silenced in various tumours, has a crucial role in tumour surveillance to suppress unwarranted cell growth and proliferation. ARF has also been linked to the DNA-damage-induced response of p53 because of its ability to inhibit murine double min...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.139
更新日期:2009-09-01 00:00:00
abstract::Ubiquitin is a post-translational modifier with proteolytic and non-proteolytic roles in many biological processes. At mitochondria, it performs regulatory homeostatic functions and contributes to mitochondrial quality control. Ubiquitin is essential for mitochondrial fusion, regulates mitochondria-ER contacts, and pa...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201338225
更新日期:2014-03-01 00:00:00
abstract::Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains unknown. Here, we present the first crystal and electron microscopy structures of a vertebrate aerolysin-like protein fr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540851
更新日期:2016-02-01 00:00:00
abstract::Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Her...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400775
更新日期:2006-10-01 00:00:00
abstract::KCC2, encoded in humans by the SLC12A5 gene, is a multifunctional neuron-specific protein initially identified as the chloride (Cl- ) extruder critical for hyperpolarizing GABAA receptor currents. Independently of its canonical function as a K-Cl cotransporter, KCC2 regulates the actin cytoskeleton via molecular inter...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948880
更新日期:2020-04-03 00:00:00
abstract::Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that for...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948925
更新日期:2020-04-03 00:00:00
abstract::GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Negative stain electron microscopy of solubilized GlpF protein re...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd022
更新日期:2000-08-01 00:00:00
abstract::Chromosomal instability (CIN) refers to the rate at which cells are unable to properly segregate whole chromosomes, leading to aneuploidy. Besides its prevalence in cancer cells and postulated implications in promoting tumorigenesis, studies in aneuploidy-prone mouse models uncovered an unanticipated link between CIN ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949248
更新日期:2020-05-06 00:00:00
abstract::SUMOylation is a post-translational modification that positively regulates monoallelic expression of the trypanosome variant surface glycoprotein (VSG). The presence of a highly SUMOylated focus associated with the nuclear body, where the VSG gene is transcribed, further suggests an important role of SUMOylation in re...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948029
更新日期:2019-12-05 00:00:00
abstract::The structure of the DNA-binding NAC domain of Arabidopsis ANAC (abscisic-acid-responsive NAC) has been determined by X-ray crystallography to 1.9A resolution (Protein Data Bank codes 1UT4 and 1UT7). This is the first structure determined for a member of the NAC family of plant-specific transcriptional regulators. NAC...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400093
更新日期:2004-03-01 00:00:00
abstract::Howy Jacobs reminisces about his many editorials and asks whether they have ever been read by anyone. ...
journal_title:EMBO reports
pub_type: 社论
doi:10.15252/embr.202050838
更新日期:2020-07-03 00:00:00
abstract::Global climate change and the increasing human population require crop varieties with higher yield and draught resistance. But meeting both goals is not an easy task for breeders and plant science. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051598
更新日期:2020-11-29 00:00:00
abstract::The mitochondrial matrix protease CLPP plays a central role in the activation of the mitochondrial unfolded protein response (UPR(mt)) in Caenorhabditis elegans Far less is known about mammalian UPR(mt) signaling, although similar roles were assumed for central players, including CLPP To better understand the mammalia...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642077
更新日期:2016-07-01 00:00:00