Abstract:
:The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in RIG-I activation remains unclear. Using in vitro-transcribed Sendai virus defective interfering RNA as a model ligand, we show that RIG-I oligomerizes on 5'-ppp dsRNA in an ATP hydrolysis-dependent and dsRNA length-dependent manner, which correlates with the strength of type-I interferon (IFN-I) activation. These results establish a clear role for the ligand-induced ATPase activity of RIG-I in the stimulation of the IFN response.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Patel JR,Jain A,Chou YY,Baum A,Ha T,García-Sastre Adoi
10.1038/embor.2013.102subject
Has Abstractpub_date
2013-09-01 00:00:00pages
780-7issue
9eissn
1469-221Xissn
1469-3178pii
embor2013102journal_volume
14pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Mitochondria play a central role in stem cell homeostasis. Reversible switching between aerobic and anaerobic metabolism is critical for stem cell quiescence, multipotency, and differentiation, as well as for cell reprogramming. However, the effect of mitochondrial dysfunction on neural stem cell (NSC) function is uns...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540982
更新日期:2015-11-01 00:00:00
abstract::Given that ligand binding is essential for the rapid internalization of epidermal growth factor receptor (EGFR), the events induced by ligand binding probably contribute to the regulation of EGFR internalization. These events include receptor dimerization, activation of intrinsic tyrosine kinase activity and autophosp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400491
更新日期:2005-10-01 00:00:00
abstract::X inactivation is the mechanism by which mammals adjust the genetic imbalance that arises from the different numbers of gene-rich X-chromosomes between the sexes. The dosage difference between XX females and XY males is functionally equalized by silencing one of the two X chromosomes in females. This dosage-compensati...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400871
更新日期:2007-01-01 00:00:00
abstract::Mutations in GRN, which encodes progranulin, are a common cause of familial frontotemporal dementia (FTD). FTD is a devastating disease characterised by neuronal loss in the frontal and temporal lobes that leads to changes in personality, behaviour and language. There are no effective treatments for this complex condi...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051668
更新日期:2020-10-05 00:00:00
abstract::Cancer-associated p53 missense mutants confer gain of function (GOF) and promote tumorigenesis by regulating crucial signaling pathways. However, the role of GOF mutant p53 in regulating DNA replication, a commonly altered pathway in cancer, is less explored. Here, we show that enhanced Cdc7-dependent replication init...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643347
更新日期:2017-11-01 00:00:00
abstract::Tie2, an endothelial cell-specific receptor kinase, has an important role in tumour angiogenesis. In an attempt to identify peptides that specifically interact with and block the Tie2 pathway, a phage-displayed peptide library was screened on a recombinant Tie2 receptor. One peptide, NLLMAAS, completely abolished the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400100
更新日期:2004-03-01 00:00:00
abstract::One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.158
更新日期:2011-09-30 00:00:00
abstract::Retinoic acid (RA) signalling ensures that vertebrate mesoderm segmentation is bilaterally synchronized, and corrects transient interferences from asymmetric left-right (L-R) signals involved in organ lateralization. Snail genes participate in both these processes and, although they are expressed symmetrically in the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400867
更新日期:2007-01-01 00:00:00
abstract::The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, and membrane or organelle dynamics. However, its filamentous properties and functions inside the mammalian cell nucleus are less well explored. We previously described transient actin assembly at...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202050758
更新日期:2020-11-05 00:00:00
abstract::Ubiquitylation is an essential post-translational modification that regulates numerous cellular processes, most notably protein degradation. Ubiquitin can itself be phosphorylated at nearly every serine, threonine, and tyrosine residue. However, the effect of this modification on ubiquitin function is largely unknown....
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540298
更新日期:2015-09-01 00:00:00
abstract::Streptococcus pneumoniae causes more than one million deaths every year, mostly of young children in developing countries, due to pneumonia, bacteremia and meningitis. The emergence and dissemination of drug-resistant pneumococcal strains, coupled to changing patterns of virulence and the inadequacy of available vacci...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kvf152
更新日期:2002-08-01 00:00:00
abstract::Embryonic stem cell (ESC) cultures display a heterogeneous gene expression profile, ranging from a pristine naïve pluripotent state to a primed epiblast state. Addition of inhibitors of GSK3β and MEK (so-called 2i conditions) pushes ESC cultures toward a more homogeneous naïve pluripotent state, but the molecular unde...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540538
更新日期:2015-07-01 00:00:00
abstract::It is widely accepted that the large trinucleotide repeat expansions observed in many neurological diseases occur during replication. However, genetic recombination has emerged as a major source of instability for tandem repeats, including minisatellites, and recent studies raise the possibility that it may also be re...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kvd031
更新日期:2000-08-01 00:00:00
abstract::The COVID-19 crisis has further highlighted the challenges for open science and data sharing in biomedical research and the need for more traceability and transparency. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051773
更新日期:2020-11-05 00:00:00
abstract::Myc is an oncoprotein transcription factor that promotes cell proliferation and apoptosis. Analysis of highly conserved elements within vertebrate Myc proteins has been instrumental in defining the functions of the Myc protein. Here, we probe the role of a highly conserved, but little studied, element within the centr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400333
更新日期:2005-02-01 00:00:00
abstract::RNA editing in plant organelles is mediated by site-specific, nuclear-encoded factors. Previous data suggested that the maintenance of these factors depends on the presence of their rapidly evolving cognate sites. The surprising ability of allotetraploid Nicotiana tabacum (tobacco) to edit a foreign site in the chloro...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400619
更新日期:2006-03-01 00:00:00
abstract::Apoptotic cell volume decrease (AVD) and exposure of phosphatidylserine (PtdSer) at the cell surface are early events in apoptosis. However, the ion channels responsible for AVD, and their relationship to PtdSer translocation and cell death are poorly understood. Real-time analysis of calcium-induced apoptosis in lymp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor722
更新日期:2003-02-01 00:00:00
abstract::The systematic recording and management of experimental data in academic life science research remains an open problem. EPFL engaged in a program of ELN/LIMS deployment 6 years ago, encountering a host of fundamental questions at the institutional level and in each single laboratory. Here, based on our experience we a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949862
更新日期:2020-03-04 00:00:00
abstract::The components and pathways that regulate and execute developmental cell death programmes in plants remain largely unknown. We have found that the PROMOTION OF CELL SURVIVAL 1 (PCS1) gene in Arabidopsis, which encodes an aspartic protease, has an important role in determining the fate of cells in embryonic development...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400357
更新日期:2005-03-01 00:00:00
abstract::Cardiac dysfunctions dramatically increase with age. Revealing a currently unknown contributor to cardiac ageing, we report the age-dependent, cardiac-specific accumulation of the lysosphingolipid sphinganine (dihydrosphingosine, DHS) as an evolutionarily conserved hallmark of the aged vertebrate heart. Mechanisticall...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201847407
更新日期:2019-04-01 00:00:00
abstract::Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201438919
更新日期:2014-08-01 00:00:00
abstract::Histone deacetylases (HDACs)-an enzyme family that deacetylates histones and non-histone proteins-are implicated in human diseases such as cancer, and the first-generation of HDAC inhibitors are now in clinical trials. Here, we report the 2.0 A resolution crystal structure of a catalytically inactive HDAC8 active-site...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401047
更新日期:2007-09-01 00:00:00
abstract::The tumour suppressor ARF (alternative reading frame), which is mutated or silenced in various tumours, has a crucial role in tumour surveillance to suppress unwarranted cell growth and proliferation. ARF has also been linked to the DNA-damage-induced response of p53 because of its ability to inhibit murine double min...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.139
更新日期:2009-09-01 00:00:00
abstract::The aggregation of the two yeast proteins Sup35p and Ure2p is widely accepted as a model for explaining the prion propagation of the phenotypes [PSI+] and [URE3], respectively. Here, we demonstrate that the propagation of [URE3] cannot simply be the consequence of generating large aggregates of Ure2p, because such agg...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf011
更新日期:2002-01-01 00:00:00
abstract::The ubiquitin ligase Highwire restrains synaptic growth and promotes evoked neurotransmission at NMJ synapses in Drosophila Highwire regulates synaptic morphology by downregulating the MAP3K Wallenda, but excess Wallenda signaling does not account for the decreased presynaptic release observed in highwire mutants. Hen...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846975
更新日期:2019-03-01 00:00:00
abstract::The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses o...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401087
更新日期:2007-11-01 00:00:00
abstract::COVID-19 highlighted the importance of science and scientists to produce vaccines, cures and diagnostics. But scientists need to be careful not to overpromise on what they can deliver. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051827
更新日期:2020-11-05 00:00:00
abstract::The biosynthesis of RNA includes its post-transcriptional modifications, and the crucial functions of these modifications have supported their conservation within all three kingdoms. For example, the modifications located within or adjacent to the anticodon of the transfer RNA (tRNA) enhance the accuracy of codon bind...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.104
更新日期:2008-07-01 00:00:00
abstract::β-arrestin 1 and 2 (also known as arrestin 2 and 3) are homologous adaptor proteins that regulate seven-transmembrane receptor trafficking and signalling. Other proteins with predicted 'arrestin-like' structural domains but lacking sequence homology have been indicated to function like β-arrestin in receptor regulatio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.187
更新日期:2013-02-01 00:00:00
abstract::The receptor-evoked Ca(2+) signal is sensed and translated by mitochondria. Physiological cytoplasmic Ca(2+) ([Ca(2+)]c) oscillations result in mitochondrial Ca(2+) ([Ca(2+)]m) oscillations, while large and sustained [Ca(2+)]c increase results in a pathologic increase in basal [Ca(2+)]m and in Ca(2+) accumulation. The...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.1002/embr.201438446
更新日期:2014-03-01 00:00:00