Optogenetic control of mitochondrial protonmotive force to impact cellular stress resistance.

abstract：:The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (DeltaN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we s...

journal_title：EMBO reports

authors： Ray PS,Grover R,Das S

更新日期：2006-04-01 00:00:00

abstract：:We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three alpha-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (alpha2/alpha2') forms...

journal_title：EMBO reports

authors： Lingel A,Simon B,Izaurralde E,Sattler M

更新日期：2005-12-01 00:00:00

abstract：:The eukaryotic GINS complex has an essential role in the initiation and elongation phases of genome duplication. It is composed of four paralogous subunits--Sld5, Psf1, Psf2 and Psf3--which are ubiquitous and evolutionarily conserved in eukaryotic organisms. Here, we report the biochemical characterization of the huma...

journal_title：EMBO reports

authors： De Falco M,Ferrari E,De Felice M,Rossi M,Hübscher U,Pisani FM

更新日期：2007-01-01 00:00:00

abstract：:Caspase-3 is a crucial component of the apoptotic machinery in many cell types. Here, we report the timescale of caspase-3 activation in single living cells undergoing apoptosis. This was achieved by measuring the extent of fluorescence resonance energy transfer within a recombinant substrate containing cyan fluoresce...

journal_title：EMBO reports

authors： Tyas L,Brophy VA,Pope A,Rivett AJ,Tavaré JM

更新日期：2000-09-01 00:00:00

abstract：:Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochro...

journal_title：EMBO reports

authors： Poulard C,Bittencourt D,Wu DY,Hu Y,Gerke DS,Stallcup MR

更新日期：2017-08-01 00:00:00

abstract：:Senescence is a stable proliferative arrest induced by various stresses such as telomere erosion, oncogenic or oxidative stress. Compelling evidence suggests that it acts as a barrier against tumour development. Describing new mechanisms that favour an escape from senescence can thus reveal new insights into tumorigen...

journal_title：EMBO reports

authors： Augert A,Payré C,de Launoit Y,Gil J,Lambeau G,Bernard D

更新日期：2009-03-01 00:00:00

abstract：:ARGONAUTE 1 (AGO1) slices endogenous messenger RNAs (mRNAs) during both microRNA (miRNA)- and short interfering RNA (siRNA)-guided post-transcriptional silencing. We have previously reported that AGO1 homeostasis is maintained through the repressive action of miR168 on AGO1 mRNA and the stabilizing effect of AGO1 prot...

journal_title：EMBO reports

authors： Mallory AC,Vaucheret H

更新日期：2009-05-01 00:00:00

abstract：:The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses o...

journal_title：EMBO reports

authors： Mak DO,Pearson JE,Loong KP,Datta S,Fernández-Mongil M,Foskett JK

更新日期：2007-11-01 00:00:00

abstract：:Chloroplast genes of higher plants are transcribed by two types of RNA polymerase that are encoded by nuclear (NEP (nuclear-encoded plastid RNA polymerase)) or plastid (PEP (plastid-encoded plastid RNA polymerase)) genomes. NEP is largely responsible for the transcription of housekeeping genes during early chloroplast...

journal_title：EMBO reports

authors： Hanaoka M,Kanamaru K,Fujiwara M,Takahashi H,Tanaka K

更新日期：2005-06-01 00:00:00

abstract：:Labmates are colleagues, friends, comforters…and competitors. ...

journal_title：EMBO reports

authors： Morriswood B,Hoeller O

更新日期：2019-07-01 00:00:00

abstract：:The orphan GluD2 receptor belongs to the ionotropic glutamate receptor family but does not bind glutamate. Ligand-gated GluD2 currents have never been evidenced, and whether GluD2 operates as an ion channel has been a long-standing question. Here, we show that GluD2 gating is triggered by type 1 metabotropic glutamate...

journal_title：EMBO reports

authors： Ady V,Perroy J,Tricoire L,Piochon C,Dadak S,Chen X,Dusart I,Fagni L,Lambolez B,Levenes C

更新日期：2014-01-01 00:00:00

abstract：:The crucial role of ubiquitin signalling in genome-integrity maintenance was first recognized in 1987 by Stefan Jentsch and Alex Varshavsky, who showed that Rad6-the repair protein involved in DNA damage tolerance-is a ubiquitin-conjugating enzyme. Although this discovery inspired extensive research and led to the dis...

journal_title：EMBO reports

authors： Lukas J

更新日期：2010-12-01 00:00:00

abstract：:Microbes exert a major impact on human health and disease by either promoting or disrupting homeostasis, in the latter instance leading to the development of infectious diseases. Such disparate outcomes are driven by the ever-evolving genetic diversity of microbes and the countervailing host responses that minimize th...

journal_title：EMBO reports

authors： Soares MP,Weiss G

更新日期：2015-11-01 00:00:00

abstract：:The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...

journal_title：EMBO reports

authors： Loenarz C,Coleman ML,Boleininger A,Schierwater B,Holland PW,Ratcliffe PJ,Schofield CJ

更新日期：2011-01-01 00:00:00

abstract：:Proteins of the Imp4/Brix superfamily are involved in ribosomal RNA processing, an essential function in all cells. We report the first structure of an Imp4/Brix superfamily protein, the Mil (for Methanothermobacter thermautotrophicus Imp4-like) protein (gene product Mth680), from the archaeon M. thermautotrophicus. T...

journal_title：EMBO reports

authors： Ng CL,Waterman D,Koonin EV,Antson AA,Ortiz-Lombardía M

更新日期：2005-02-01 00:00:00

abstract：:Ageing is an unavoidable corollary to being alive; the most intuitive interpretation of ageing being that it is the consequence of progressive body degeneration. In agreement with this, current models propose that ageing occurs through a stepwise accumulation of DNA damage, which ultimately limits the regenerative cap...

journal_title：EMBO reports

authors： Fernandez-Capetillo O

更新日期：2010-01-01 00:00:00

abstract：:One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site ...

journal_title：EMBO reports

authors： Jacome A,Fernandez-Capetillo O

更新日期：2011-09-30 00:00:00

abstract：:Caspase malfunction in stem cells often precedes the appearance and progression of multiple types of cancer, including human colorectal cancer. However, the caspase-dependent regulation of intestinal stem cell properties remains poorly understood. Here, we demonstrate that Dronc, the Drosophila ortholog of caspase-9/2...

journal_title：EMBO reports

authors： Arthurton L,Nahotko DA,Alonso J,Wendler F,Baena-Lopez LA

更新日期：2020-11-01 00:00:00

abstract：:In an attempt to further increase transgene expression levels in plants over and above the enhancement obtained with a 5' untranslated leader intron, three different maize introns were inserted at three different positions within the coding sequence of the luciferase reporter gene. Constructs were transformed into mai...

journal_title：EMBO reports

authors： Bourdon V,Harvey A,Lonsdale DM

更新日期：2001-05-01 00:00:00

abstract：:Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage-derived EVs through an M.tb SecA2-dependent pathway and that EVs released from M.tb-infec...

journal_title：EMBO reports

authors： Cheng Y,Schorey JS

更新日期：2019-03-01 00:00:00

abstract：:TOR is a conserved serine/threonine kinase that responds to nutrients, growth factors, the bioenergetic status of the cell and cellular stress to control growth, metabolism and ageing. A diverse group of small GTPases including Rheb, Rag, Rac1, RalA and Ryh1 play a variety of roles in the regulation of TOR. For exampl...

journal_title：EMBO reports

authors： Durán RV,Hall MN

更新日期：2012-02-01 00:00:00

abstract：:Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Her...

journal_title：EMBO reports

authors： Löwenberg M,Verhaar AP,Bilderbeek J,Marle Jv,Buttgereit F,Peppelenbosch MP,van Deventer SJ,Hommes DW

更新日期：2006-10-01 00:00:00

abstract：:The delta2 glutamate receptor (GluRdelta2) has a crucial role in cerebellar functions; disruption of GluRdelta2 alleles in mice (delta2(-/-)) impairs synapse formation and long-term depression, which is thought to underlie motor learning in the cerebellum, and consequently leads to motor discoordination. However, it h...

journal_title：EMBO reports

authors： Hirai H,Miyazaki T,Kakegawa W,Matsuda S,Mishina M,Watanabe M,Yuzaki M

更新日期：2005-01-01 00:00:00

abstract：:Nuclear-pore complexes (NPCs) are large protein channels that span the nuclear envelope (NE), which is a double membrane that encloses the nuclear genome of eukaryotes. Each of the typically 2,000-4,000 pores in the NE of vertebrate cells is composed of multiple copies of 30 different proteins known as nucleoporins. T...

journal_title：EMBO reports

authors： Capelson M,Hetzer MW

更新日期：2009-07-01 00:00:00

abstract：:Short interfering RNAs (siRNAs) are short (21-23 nt) double-stranded RNAs that direct the sequence-specific degradation of corresponding mRNAs, resulting in suppression of gene activity. siRNAs are powerful tools for gene functional analysis in mammals. Chemically synthesized siRNAs permit transient gene repression bu...

journal_title：EMBO reports

authors： Fritsch L,Martinez LA,Sekhri R,Naguibneva I,Gérard M,Vandromme M,Schaeffer L,Harel-Bellan A

更新日期：2004-02-01 00:00:00

abstract：:The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the ce...

journal_title：EMBO reports

authors： Okada M,Takezawa S,Mezaki Y,Yamaoka I,Takada I,Kitagawa H,Kato S

更新日期：2008-06-01 00:00:00

abstract：:Although iron is required for cell proliferation, iron-dependent programmed cell death serves as a critical barrier to tumor growth and metastasis. Emerging evidence suggests that iron-mediated lipid oxidation also facilitates immune eradication of cancer. However, the regulatory mechanisms of iron metabolism in cance...

journal_title：EMBO reports

authors： Song J,Liu T,Yin Y,Zhao W,Lin Z,Yin Y,Lu D,You F

更新日期：2021-01-04 00:00:00

abstract：:The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that th...

journal_title：EMBO reports

authors： Bomar MG,Pai MT,Tzeng SR,Li SS,Zhou P

更新日期：2007-03-01 00:00:00

abstract：:The mechanism by which YopP simultaneously inhibits mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB pathways has been elusive. Ectopic expression of YopP inhibits the activity and ubiquitination of a complex consisting of overexpressed TGF-beta-activated kinase 1 (TAK1) and its subunit TAK1-binding p...

journal_title：EMBO reports

authors： Thiefes A,Wolf A,Doerrie A,Grassl GA,Matsumoto K,Autenrieth I,Bohn E,Sakurai H,Niedenthal R,Resch K,Kracht M

更新日期：2006-08-01 00:00:00

abstract：:The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt...

journal_title：EMBO reports

authors： Mendes CS,Arama E,Brown S,Scherr H,Srivastava M,Bergmann A,Steller H,Mollereau B

更新日期：2006-09-01 00:00:00