Optogenetic control of mitochondrial protonmotive force to impact cellular stress resistance.


:Mitochondrial respiration generates an electrochemical proton gradient across the mitochondrial inner membrane called protonmotive force (PMF) to drive diverse functions and synthesize ATP. Current techniques to manipulate the PMF are limited to its dissipation; yet, there is no precise and reversible method to increase the PMF. To address this issue, we aimed to use an optogenetic approach and engineered a mitochondria-targeted light-activated proton pump that we name mitochondria-ON (mtON) to selectively increase the PMF in Caenorhabditis elegans. Here we show that mtON photoactivation increases the PMF in a dose-dependent manner, supports ATP synthesis, increases resistance to mitochondrial toxins, and modulates energy-sensing behavior. Moreover, transient mtON activation during hypoxic preconditioning prevents the well-characterized adaptive response of hypoxia resistance. Our results show that optogenetic manipulation of the PMF is a powerful tool to modulate metabolism and cell signaling.




EMBO reports


Berry BJ,Trewin AJ,Milliken AS,Baldzizhar A,Amitrano AM,Lim Y,Kim M,Wojtovich AP




Has Abstract


2020-04-03 00:00:00












  • Two internal ribosome entry sites mediate the translation of p53 isoforms.

    abstract::The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (DeltaN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we s...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Ray PS,Grover R,Das S

    更新日期:2006-04-01 00:00:00

  • The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition.

    abstract::We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three alpha-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (alpha2/alpha2') forms...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Lingel A,Simon B,Izaurralde E,Sattler M

    更新日期:2005-12-01 00:00:00

  • The human GINS complex binds to and specifically stimulates human DNA polymerase alpha-primase.

    abstract::The eukaryotic GINS complex has an essential role in the initiation and elongation phases of genome duplication. It is composed of four paralogous subunits--Sld5, Psf1, Psf2 and Psf3--which are ubiquitous and evolutionarily conserved in eukaryotic organisms. Here, we report the biochemical characterization of the huma...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: De Falco M,Ferrari E,De Felice M,Rossi M,Hübscher U,Pisani FM

    更新日期:2007-01-01 00:00:00

  • Rapid caspase-3 activation during apoptosis revealed using fluorescence-resonance energy transfer.

    abstract::Caspase-3 is a crucial component of the apoptotic machinery in many cell types. Here, we report the timescale of caspase-3 activation in single living cells undergoing apoptosis. This was achieved by measuring the extent of fluorescence resonance energy transfer within a recombinant substrate containing cyan fluoresce...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Tyas L,Brophy VA,Pope A,Rivett AJ,Tavaré JM

    更新日期:2000-09-01 00:00:00

  • A post-translational modification switch controls coactivator function of histone methyltransferases G9a and GLP.

    abstract::Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochro...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Poulard C,Bittencourt D,Wu DY,Hu Y,Gerke DS,Stallcup MR

    更新日期:2017-08-01 00:00:00

  • The M-type receptor PLA2R regulates senescence through the p53 pathway.

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    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Augert A,Payré C,de Launoit Y,Gil J,Lambeau G,Bernard D

    更新日期:2009-03-01 00:00:00

  • ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways.

    abstract::ARGONAUTE 1 (AGO1) slices endogenous messenger RNAs (mRNAs) during both microRNA (miRNA)- and short interfering RNA (siRNA)-guided post-transcriptional silencing. We have previously reported that AGO1 homeostasis is maintained through the repressive action of miR168 on AGO1 mRNA and the stabilizing effect of AGO1 prot...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Mallory AC,Vaucheret H

    更新日期:2009-05-01 00:00:00

  • Rapid ligand-regulated gating kinetics of single inositol 1,4,5-trisphosphate receptor Ca2+ release channels.

    abstract::The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses o...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Mak DO,Pearson JE,Loong KP,Datta S,Fernández-Mongil M,Foskett JK

    更新日期:2007-11-01 00:00:00

  • Glutamyl-tRNA mediates a switch in RNA polymerase use during chloroplast biogenesis.

    abstract::Chloroplast genes of higher plants are transcribed by two types of RNA polymerase that are encoded by nuclear (NEP (nuclear-encoded plastid RNA polymerase)) or plastid (PEP (plastid-encoded plastid RNA polymerase)) genomes. NEP is largely responsible for the transcription of housekeeping genes during early chloroplast...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Hanaoka M,Kanamaru K,Fujiwara M,Takahashi H,Tanaka K

    更新日期:2005-06-01 00:00:00

  • The rat race.

    abstract::Labmates are colleagues, friends, comforters…and competitors. ...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Morriswood B,Hoeller O

    更新日期:2019-07-01 00:00:00

  • Type 1 metabotropic glutamate receptors (mGlu1) trigger the gating of GluD2 delta glutamate receptors.

    abstract::The orphan GluD2 receptor belongs to the ionotropic glutamate receptor family but does not bind glutamate. Ligand-gated GluD2 currents have never been evidenced, and whether GluD2 operates as an ion channel has been a long-standing question. Here, we show that GluD2 gating is triggered by type 1 metabotropic glutamate...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Ady V,Perroy J,Tricoire L,Piochon C,Dadak S,Chen X,Dusart I,Fagni L,Lambolez B,Levenes C

    更新日期:2014-01-01 00:00:00

  • The interface between the ubiquitin family and the DNA damage response.

    abstract::The crucial role of ubiquitin signalling in genome-integrity maintenance was first recognized in 1987 by Stefan Jentsch and Alex Varshavsky, who showed that Rad6-the repair protein involved in DNA damage tolerance-is a ubiquitin-conjugating enzyme. Although this discovery inspired extensive research and led to the dis...

    journal_title:EMBO reports



    authors: Lukas J

    更新日期:2010-12-01 00:00:00

  • The Iron age of host-microbe interactions.

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    journal_title:EMBO reports

    pub_type: 杂志文章,评审


    authors: Soares MP,Weiss G

    更新日期:2015-11-01 00:00:00

  • The hypoxia-inducible transcription factor pathway regulates oxygen sensing in the simplest animal, Trichoplax adhaerens.

    abstract::The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Loenarz C,Coleman ML,Boleininger A,Schierwater B,Holland PW,Ratcliffe PJ,Schofield CJ

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    abstract::Proteins of the Imp4/Brix superfamily are involved in ribosomal RNA processing, an essential function in all cells. We report the first structure of an Imp4/Brix superfamily protein, the Mil (for Methanothermobacter thermautotrophicus Imp4-like) protein (gene product Mth680), from the archaeon M. thermautotrophicus. T...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Ng CL,Waterman D,Koonin EV,Antson AA,Ortiz-Lombardía M

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    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Fernandez-Capetillo O

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    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Jacome A,Fernandez-Capetillo O

    更新日期:2011-09-30 00:00:00

  • Non-apoptotic caspase activation preserves Drosophila intestinal progenitor cells in quiescence.

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    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Arthurton L,Nahotko DA,Alonso J,Wendler F,Baena-Lopez LA

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  • Introns and their positions affect the translational activity of mRNA in plant cells.

    abstract::In an attempt to further increase transgene expression levels in plants over and above the enhancement obtained with a 5' untranslated leader intron, three different maize introns were inserted at three different positions within the coding sequence of the luciferase reporter gene. Constructs were transformed into mai...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Bourdon V,Harvey A,Lonsdale DM

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  • Extracellular vesicles deliver Mycobacterium RNA to promote host immunity and bacterial killing.

    abstract::Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage-derived EVs through an M.tb SecA2-dependent pathway and that EVs released from M.tb-infec...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Cheng Y,Schorey JS

    更新日期:2019-03-01 00:00:00

  • Regulation of TOR by small GTPases.

    abstract::TOR is a conserved serine/threonine kinase that responds to nutrients, growth factors, the bioenergetic status of the cell and cellular stress to control growth, metabolism and ageing. A diverse group of small GTPases including Rheb, Rag, Rac1, RalA and Ryh1 play a variety of roles in the regulation of TOR. For exampl...

    journal_title:EMBO reports

    pub_type: 杂志文章,评审


    authors: Durán RV,Hall MN

    更新日期:2012-02-01 00:00:00

  • Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN.

    abstract::Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Her...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Löwenberg M,Verhaar AP,Bilderbeek J,Marle Jv,Buttgereit F,Peppelenbosch MP,van Deventer SJ,Hommes DW

    更新日期:2006-10-01 00:00:00

  • Rescue of abnormal phenotypes of the delta2 glutamate receptor-null mice by mutant delta2 transgenes.

    abstract::The delta2 glutamate receptor (GluRdelta2) has a crucial role in cerebellar functions; disruption of GluRdelta2 alleles in mice (delta2(-/-)) impairs synapse formation and long-term depression, which is thought to underlie motor learning in the cerebellum, and consequently leads to motor discoordination. However, it h...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Hirai H,Miyazaki T,Kakegawa W,Matsuda S,Mishina M,Watanabe M,Yuzaki M

    更新日期:2005-01-01 00:00:00

  • The role of nuclear pores in gene regulation, development and disease.

    abstract::Nuclear-pore complexes (NPCs) are large protein channels that span the nuclear envelope (NE), which is a double membrane that encloses the nuclear genome of eukaryotes. Each of the typically 2,000-4,000 pores in the NE of vertebrate cells is composed of multiple copies of 30 different proteins known as nucleoporins. T...

    journal_title:EMBO reports

    pub_type: 杂志文章,评审


    authors: Capelson M,Hetzer MW

    更新日期:2009-07-01 00:00:00

  • Conditional gene knock-down by CRE-dependent short interfering RNAs.

    abstract::Short interfering RNAs (siRNAs) are short (21-23 nt) double-stranded RNAs that direct the sequence-specific degradation of corresponding mRNAs, resulting in suppression of gene activity. siRNAs are powerful tools for gene functional analysis in mammals. Chemically synthesized siRNAs permit transient gene repression bu...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Fritsch L,Martinez LA,Sekhri R,Naguibneva I,Gérard M,Vandromme M,Schaeffer L,Harel-Bellan A

    更新日期:2004-02-01 00:00:00

  • Switching of chromatin-remodelling complexes for oestrogen receptor-alpha.

    abstract::The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the ce...

    journal_title:EMBO reports

    pub_type: 杂志文章,收录出版


    authors: Okada M,Takezawa S,Mezaki Y,Yamaoka I,Takada I,Kitagawa H,Kato S

    更新日期:2008-06-01 00:00:00

  • The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity.

    abstract::Although iron is required for cell proliferation, iron-dependent programmed cell death serves as a critical barrier to tumor growth and metastasis. Emerging evidence suggests that iron-mediated lipid oxidation also facilitates immune eradication of cancer. However, the regulatory mechanisms of iron metabolism in cance...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Song J,Liu T,Yin Y,Zhao W,Lin Z,Yin Y,Lu D,You F

    更新日期:2021-01-04 00:00:00

  • Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta.

    abstract::The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that th...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Bomar MG,Pai MT,Tzeng SR,Li SS,Zhou P

    更新日期:2007-03-01 00:00:00

  • The Yersinia enterocolitica effector YopP inhibits host cell signalling by inactivating the protein kinase TAK1 in the IL-1 signalling pathway.

    abstract::The mechanism by which YopP simultaneously inhibits mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB pathways has been elusive. Ectopic expression of YopP inhibits the activity and ubiquitination of a complex consisting of overexpressed TGF-beta-activated kinase 1 (TAK1) and its subunit TAK1-binding p...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Thiefes A,Wolf A,Doerrie A,Grassl GA,Matsumoto K,Autenrieth I,Bohn E,Sakurai H,Niedenthal R,Resch K,Kracht M

    更新日期:2006-08-01 00:00:00

  • Cytochrome c-d regulates developmental apoptosis in the Drosophila retina.

    abstract::The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt...

    journal_title:EMBO reports

    pub_type: 杂志文章


    authors: Mendes CS,Arama E,Brown S,Scherr H,Srivastava M,Bergmann A,Steller H,Mollereau B

    更新日期:2006-09-01 00:00:00