Abstract:
:The Polycomb group (PcG) of proteins conveys epigenetic inheritance of repressed transcriptional states. In Drosophila, the Polycomb repressive complex 1 (PRC1) maintains the silent state by inhibiting the transcription machinery and chromatin remodelling at core promoters. Using immunoprecipitation of in vivo formaldehyde-fixed chromatin in phenotypically diverse cultured cell lines, we have mapped PRC1 components, the histone methyl transferase (HMT) Enhancer of zeste (E(z)) and histone H3 modifications in active and inactive PcG-controlled regions. We show that PRC1 components are present in both cases, but at different levels. In particular, active target promoters are nearly devoid of E(z) and Polycomb. Moreover, repressed regions are trimethylated at lysines 9 and 27, suggesting that these histone modifications represent a mark for inactive PcG-controlled regions. These PcG-specific repressive marks are maintained by the action of the E(z) HMT, an enzyme that has an important role not only in establishing but also in maintaining PcG repression.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Breiling A,O'Neill LP,D'Eliseo D,Turner BM,Orlando Vdoi
10.1038/sj.embor.7400260keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
976-82issue
10eissn
1469-221Xissn
1469-3178pii
7400260journal_volume
5pub_type
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