Loss of non-canonical KCC2 functions promotes developmental apoptosis of cortical projection neurons.

abstract：:The thymus provides a specialized microenvironment in which distinct subsets of thymic epithelial cells (TECs) support T-cell development. Here, we describe the significance of cortical TECs (cTECs) in T-cell development, using a newly established mouse model of cTEC deficiency. The deficiency of mature cTECs caused a...

journal_title：EMBO reports

authors： Nitta T,Muro R,Shimizu Y,Nitta S,Oda H,Ohte Y,Goto M,Yanobu-Takanashi R,Narita T,Takayanagi H,Yasuda H,Okamura T,Murata S,Suzuki H

更新日期：2015-05-01 00:00:00

abstract：:The crystal structure of the ligand-binding domain of RARbeta, a suspect tumour suppressor, reveals important features that distinguish it from the two other RAR isotypes. The most striking difference is an extra cavity allowing RARbeta to bind more bulky agonists. Accordingly, we identified a ligand that shows RARbet...

journal_title：EMBO reports

authors： Germain P,Kammerer S,Pérez E,Peluso-Iltis C,Tortolani D,Zusi FC,Starrett J,Lapointe P,Daris JP,Marinier A,de Lera AR,Rochel N,Gronemeyer H

更新日期：2004-09-01 00:00:00

abstract：:The Drosophila immune system is able to discriminate between classes of bacteria. Detection of Gram-positive bacteria involves a complex of two pattern recognition receptors: peptidoglycan recognition protein SA (PGRP-SA) and Gram-negative binding protein 1 (GNBP1). These activate the Toll signalling pathway. To defin...

journal_title：EMBO reports

authors： Filipe SR,Tomasz A,Ligoxygakis P

更新日期：2005-04-01 00:00:00

abstract：:Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochro...

journal_title：EMBO reports

authors： Poulard C,Bittencourt D,Wu DY,Hu Y,Gerke DS,Stallcup MR

更新日期：2017-08-01 00:00:00

abstract：:Acetaminophen/paracetamol-induced liver failure--which is induced by the binding of reactive metabolites to mitochondrial proteins and their disruption--is exacerbated by fasting. As fasting promotes SIRT3-mediated mitochondrial-protein deacetylation and acetaminophen metabolites bind to lysine residues, we investigat...

journal_title：EMBO reports

authors： Lu Z,Bourdi M,Li JH,Aponte AM,Chen Y,Lombard DB,Gucek M,Pohl LR,Sack MN

更新日期：2011-07-01 00:00:00

abstract：:Cells suffer necrotic death when exposed to extreme environmental conditions, adverse and excessive stimuli, or when deleterious mutations are encoded in their genetic material. Unlike apoptosis, which involves a highly regulated and elaborate network of biochemical events and cascades, necrosis has been considered ge...

journal_title：EMBO reports

authors： Syntichaki P,Tavernarakis N

更新日期：2002-07-01 00:00:00

abstract：:SUMOylation is a post-translational modification that positively regulates monoallelic expression of the trypanosome variant surface glycoprotein (VSG). The presence of a highly SUMOylated focus associated with the nuclear body, where the VSG gene is transcribed, further suggests an important role of SUMOylation in re...

journal_title：EMBO reports

authors： Saura A,Iribarren PA,Rojas-Barros D,Bart JM,López-Farfán D,Andrés-León E,Vidal-Cobo I,Boehm C,Alvarez VE,Field MC,Navarro M

更新日期：2019-12-05 00:00:00

abstract：:Impairment of PINK1/parkin-mediated mitophagy is currently proposed to be the molecular basis of mitochondrial abnormality in Parkinson's disease (PD). We here demonstrate that PINK1 directly phosphorylates Drp1 on S616. Drp1S616 phosphorylation is significantly reduced in cells and mouse tissues deficient for PINK1, ...

journal_title：EMBO reports

authors： Han H,Tan J,Wang R,Wan H,He Y,Yan X,Guo J,Gao Q,Li J,Shang S,Chen F,Tian R,Liu W,Liao L,Tang B,Zhang Z

更新日期：2020-08-05 00:00:00

abstract：:Stat1 (signal transducer and activator of transcription 1) regulates transcription in response to the type I interferons IFN-alpha and IFN-beta, either in its dimerized form or as a subunit of the interferon-stimulated gene factor 3 (Isgf3) complex (consisting of Stat1, Stat2 and interferon-regulating factor 9). Full-...

journal_title：EMBO reports

authors： Pilz A,Ramsauer K,Heidari H,Leitges M,Kovarik P,Decker T

更新日期：2003-04-01 00:00:00

abstract：:Cohesin cofactors regulate the loading, maintenance, and release of cohesin complexes from chromosomes during mitosis but little is known on their role during vertebrate meiosis. One such cofactor is PDS5, which exists as two paralogs in somatic and germline cells, PDS5A and PDS5B, with unclear functions. Here, we hav...

journal_title：EMBO reports

authors： Viera A,Berenguer I,Ruiz-Torres M,Gómez R,Guajardo A,Barbero JL,Losada A,Suja JA

更新日期：2020-06-04 00:00:00

abstract：:Alternative RNA processing of LMNA pre-mRNA produces three main protein isoforms, that is, lamin A, progerin, and lamin C. De novo mutations that favor the expression of progerin over lamin A lead to Hutchinson-Gilford progeria syndrome (HGPS), providing support for the involvement of LMNA processing in pathological a...

journal_title：EMBO reports

authors： Lopez-Mejia IC,de Toledo M,Chavey C,Lapasset L,Cavelier P,Lopez-Herrera C,Chebli K,Fort P,Beranger G,Fajas L,Amri EZ,Casas F,Tazi J

更新日期：2014-05-01 00:00:00

abstract：:Histone deacetylases (HDACs)-an enzyme family that deacetylates histones and non-histone proteins-are implicated in human diseases such as cancer, and the first-generation of HDAC inhibitors are now in clinical trials. Here, we report the 2.0 A resolution crystal structure of a catalytically inactive HDAC8 active-site...

journal_title：EMBO reports

authors： Vannini A,Volpari C,Gallinari P,Jones P,Mattu M,Carfí A,De Francesco R,Steinkühler C,Di Marco S

更新日期：2007-09-01 00:00:00

abstract：:Phosphoinositides have crucial roles in cellular controls, many of which have been established through the use of small-molecule inhibitors. Here, we describe YM201636, a potent inhibitor of the mammalian class III phosphatidylinositol phosphate kinase PIKfyve, which synthesizes phosphatidylinositol 3,5-bisphosphate. ...

journal_title：EMBO reports

authors： Jefferies HB,Cooke FT,Jat P,Boucheron C,Koizumi T,Hayakawa M,Kaizawa H,Ohishi T,Workman P,Waterfield MD,Parker PJ

更新日期：2008-02-01 00:00:00

abstract：:Caspase-3 is a crucial component of the apoptotic machinery in many cell types. Here, we report the timescale of caspase-3 activation in single living cells undergoing apoptosis. This was achieved by measuring the extent of fluorescence resonance energy transfer within a recombinant substrate containing cyan fluoresce...

journal_title：EMBO reports

authors： Tyas L,Brophy VA,Pope A,Rivett AJ,Tavaré JM

更新日期：2000-09-01 00:00:00

abstract：:Embryonic stem cell (ESC) cultures display a heterogeneous gene expression profile, ranging from a pristine naïve pluripotent state to a primed epiblast state. Addition of inhibitors of GSK3β and MEK (so-called 2i conditions) pushes ESC cultures toward a more homogeneous naïve pluripotent state, but the molecular unde...

journal_title：EMBO reports

authors： Welling M,Chen HH,Muñoz J,Musheev MU,Kester L,Junker JP,Mischerikow N,Arbab M,Kuijk E,Silberstein L,Kharchenko PV,Geens M,Niehrs C,van de Velde H,van Oudenaarden A,Heck AJ,Geijsen N

更新日期：2015-07-01 00:00:00

abstract：:Ligand-dependent transcriptional activation of retinoic acid receptors (RARs) is a multistep process culminating in the formation of a multimeric co-activator complex on regulated promoters. Several co-activator complexes harbor an acetyl transferase activity, which is required for retinoid-induced transcription of re...

journal_title：EMBO reports

authors： Lefebvre B,Ozato K,Lefebvre P

更新日期：2002-04-01 00:00:00

abstract：:Very little is known about the biogenesis and assembly of oligomeric membrane proteins. In this study, the biogenesis of KcsA, a prokaryotic homotetrameric potassium channel, is investigated. Using in vivo pulse-chase experiments, both the monomeric and tetrameric form could be identified. The conversion of monomers i...

journal_title：EMBO reports

authors： van Dalen A,Schrempf H,Killian JA,de Kruijff B

更新日期：2000-10-01 00:00:00

abstract：:The Wnt signaling pathway can be grouped into two classes, the β-catenin-dependent and β-catenin-independent pathways. Wnt5a signaling through a β-catenin-independent pathway promotes microtubule (MT) remodeling during cell-substrate adhesion, cell migration, and planar cell polarity formation. Although Wnt5a signalin...

journal_title：EMBO reports

authors： Kikuchi K,Nakamura A,Arata M,Shi D,Nakagawa M,Tanaka T,Uemura T,Fujimori T,Kikuchi A,Uezu A,Sakamoto Y,Nakanishi H

更新日期：2018-07-01 00:00:00

abstract：:We have designed a doxycycline-regulated form of the H1 promoter of RNA polymerase III that allows the inducible knockdown of gene expression by small interfering RNAs (siRNAs). As a proof-of-principle, we have targeted beta-catenin in colorectal cancer (CRC) cells. T-cell factor (TCF) target-gene expression is induce...

journal_title：EMBO reports

authors： van de Wetering M,Oving I,Muncan V,Pon Fong MT,Brantjes H,van Leenen D,Holstege FC,Brummelkamp TR,Agami R,Clevers H

更新日期：2003-06-01 00:00:00

abstract：:Senescence is a stable proliferative arrest induced by various stresses such as telomere erosion, oncogenic or oxidative stress. Compelling evidence suggests that it acts as a barrier against tumour development. Describing new mechanisms that favour an escape from senescence can thus reveal new insights into tumorigen...

journal_title：EMBO reports

authors： Augert A,Payré C,de Launoit Y,Gil J,Lambeau G,Bernard D

更新日期：2009-03-01 00:00:00

abstract：:The human XAB1/MBDin GTPase and its close homologues form one of the ten phylogenetically distinct families of the SIMIBI (after signal recognition particle, MinD and BioD) class of phosphate-binding loop NTPases. The genomic context and the partners identified for the archaeal and eukaryotic homologues indicate that ...

journal_title：EMBO reports

authors： Gras S,Chaumont V,Fernandez B,Carpentier P,Charrier-Savournin F,Schmitt S,Pineau C,Flament D,Hecker A,Forterre P,Armengaud J,Housset D

更新日期：2007-06-01 00:00:00

abstract：:Growth plasticity is a key mechanism by which plants adapt to the ever-changing environmental conditions. Since growth is a high-energy-demanding and irreversible process, it is expected to be regulated by the integration of endogenous energy status as well as environmental conditions. Here, we show that trehalose-6-p...

journal_title：EMBO reports

authors： Hwang G,Kim S,Cho JY,Paik I,Kim JI,Oh E

更新日期：2019-10-04 00:00:00

abstract：:Centromeric constitutive heterochromatin is marked by DNA methylation and dimethylated histone H3 Lys 9 (H3K9me2) in Arabidopsis. RNA-directed DNA methylation (RdDM) is a process that uses 24-nucleotide (nt) small interfering RNAs (siRNAs) to induce de novo methylation to its homologous DNA sequences. Despite the pres...

journal_title：EMBO reports

authors： Schoft VK,Chumak N,Mosiolek M,Slusarz L,Komnenovic V,Brownfield L,Twell D,Kakutani T,Tamaru H

更新日期：2009-09-01 00:00:00

abstract：:In an attempt to further increase transgene expression levels in plants over and above the enhancement obtained with a 5' untranslated leader intron, three different maize introns were inserted at three different positions within the coding sequence of the luciferase reporter gene. Constructs were transformed into mai...

journal_title：EMBO reports

authors： Bourdon V,Harvey A,Lonsdale DM

更新日期：2001-05-01 00:00:00

abstract：:Scrapie, bovine spongiform encephalopathy and chronic wasting disease are orally communicable, transmissible spongiform encephalopathies (TSEs). As zoonotic transmissions of TSE agents may pose a risk to human health, the identification of reservoirs for infectivity in animal tissues and their exclusion from human con...

journal_title：EMBO reports

authors： Thomzig A,Kratzel C,Lenz G,Krüger D,Beekes M

更新日期：2003-05-01 00:00:00

abstract：:Synaptic vesicles fuse with the plasma membrane in response to Ca(2+) influx, thereby releasing neurotransmitters into the synaptic cleft. The protein machinery that mediates this process, consisting of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and regulatory proteins, is well kno...

journal_title：EMBO reports

authors： Bharat TA,Malsam J,Hagen WJ,Scheutzow A,Söllner TH,Briggs JA

更新日期：2014-03-01 00:00:00

abstract：:The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...

journal_title：EMBO reports

authors： Loenarz C,Coleman ML,Boleininger A,Schierwater B,Holland PW,Ratcliffe PJ,Schofield CJ

更新日期：2011-01-01 00:00:00

abstract：:In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription ...

journal_title：EMBO reports

authors： Goriaux C,Desset S,Renaud Y,Vaury C,Brasset E

更新日期：2014-04-01 00:00:00

abstract：:The systematic recording and management of experimental data in academic life science research remains an open problem. EPFL engaged in a program of ELN/LIMS deployment 6 years ago, encountering a host of fundamental questions at the institutional level and in each single laboratory. Here, based on our experience we a...

journal_title：EMBO reports

authors： Argento N

更新日期：2020-03-04 00:00:00

abstract：:The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...

journal_title：EMBO reports

authors： Patel JR,Jain A,Chou YY,Baum A,Ha T,García-Sastre A

更新日期：2013-09-01 00:00:00