Abstract:
:The Wnt signaling pathway can be grouped into two classes, the β-catenin-dependent and β-catenin-independent pathways. Wnt5a signaling through a β-catenin-independent pathway promotes microtubule (MT) remodeling during cell-substrate adhesion, cell migration, and planar cell polarity formation. Although Wnt5a signaling and MT remodeling are known to form an interdependent regulatory loop, the underlying mechanism remains unknown. Here we show that in HeLa cells, the paralogous MT-associated proteins Map7 and Map7D1 (Map7/7D1) form an interdependent regulatory loop with Disheveled, the critical signal transducer in Wnt signaling. Map7/7D1 bind to Disheveled, direct its cortical localization, and facilitate the cortical targeting of MT plus-ends in response to Wnt5a signaling. Wnt5a signaling also promotes Map7/7D1 movement toward MT plus-ends, and depletion of the Kinesin-1 member Kif5b abolishes the Map7/7D1 dynamics and Disheveled localization. Furthermore, Disheveled stabilizes Map7/7D1. Intriguingly, Map7/7D1 and its Drosophila ortholog, Ensconsin show planar-polarized distribution in both mouse and fly epithelia, and Ensconsin influences proper localization of Drosophila Disheveled in pupal wing cells. These results suggest that the role of Map7/7D1/Ensconsin in Disheveled localization is evolutionarily conserved.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Kikuchi K,Nakamura A,Arata M,Shi D,Nakagawa M,Tanaka T,Uemura T,Fujimori T,Kikuchi A,Uezu A,Sakamoto Y,Nakanishi Hdoi
10.15252/embr.201745471subject
Has Abstractpub_date
2018-07-01 00:00:00issue
7eissn
1469-221Xissn
1469-3178pii
embr.201745471journal_volume
19pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Sirtuins (SIRTs) are a class of lysine deacylases that regulate cellular metabolism and energy homeostasis. Although sirtuins have been proposed to function in nutrient sensing and signaling, the underlying mechanism remains elusive. SIRT7, a histone H3K18-specific deacetylase, epigenetically controls mitochondria bio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846377
更新日期:2018-12-01 00:00:00
abstract::Several studies have shown that ribosomal proteins (RPs) are important mediators of p53 activation in response to nucleolar disruption; however, the pathways that control this signalling function of RPs are currently unknown. We have recently shown that RPs are targets for the ubiquitin-like molecule NEDD8, and that N...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.178
更新日期:2009-10-01 00:00:00
abstract::Sepsis, or blood poisoning, is a savage response of the body to infection. It can lead to organ failure, blood pressure decline, heart failure, and coma. Between 20 and 30 million people suffer from sepsis each year, leading to 8 million deaths. Although certain people are more at risk than others (young children, eld...
journal_title:EMBO reports
pub_type: 评论,新闻
doi:10.15252/embr.201949193
更新日期:2020-01-07 00:00:00
abstract::Expression of the tight junction protein junctional adhesion molecule-A (JAM-A) has been linked to proliferation and tumour progression. However, a direct role for JAM-A in regulating proliferative processes has not been shown. By using complementary in vivo and in vitro approaches, we demonstrate that JAM-A restricts...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.16
更新日期:2011-04-01 00:00:00
abstract::Microglia are specialized parenchymal-resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846171
更新日期:2018-11-01 00:00:00
abstract::Mechanisms that guide directional migration of neuroblasts from the subventricular zone (SVZ) are not well understood. We report here that endogenous electric currents serve as a guidance cue for neuroblast migration. We identify the existence of naturally occurring electric currents (1.5±0.6 μA/cm(2), average field s...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.215
更新日期:2013-02-01 00:00:00
abstract::PTIP regulates gene transcription by controlling the methylation of histone H3, and also has important roles in cellular responses to DNA damage or to perturbed DNA replication. The available data suggest that the functions of PTIP in transcription and preserving genome stability might be independent and mediated by f...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2009.21
更新日期:2009-03-01 00:00:00
abstract::In Drosophila melanogaster males, X-chromosome monosomy is compensated by chromosome-wide transcription activation. We found that complete dosage compensation during embryogenesis takes surprisingly long and is incomplete even after 10 h of development. Although the activating dosage compensation complex (DCC) associa...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948138
更新日期:2019-08-01 00:00:00
abstract::Somatic hypermutation of immunoglobulin variable genes, which increases antibody diversity, is initiated by the activation-induced cytosine deaminase (AID) protein. The current DNA-deamination model posits that AID deaminates cytosine to uracil in DNA, and that mutations are generated by DNA polymerases during replica...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400582
更新日期:2005-12-01 00:00:00
abstract::Several mechanisms have been proposed for the synthesis of substrate-linked ubiquitin chains. HECT ligases directly catalyse protein ubiquitination and have been found to non-covalently interact with ubiquitin. We report crystal structures of the Nedd4 HECT domain, alone and in complex with ubiquitin, which show a new...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.21
更新日期:2011-04-01 00:00:00
abstract::Comment on "Close loop peer review" by Michael Hill. ...
journal_title:EMBO reports
pub_type: 评论,信件
doi:10.15252/embr.201948313
更新日期:2019-08-01 00:00:00
abstract::Pair-rule genes in Drosophila integrate the positional information provided by the transcription factor gradients of gap genes and set the framework for the expression of the segment polarity genes. Pair-rule genes are also expressed during the segmentation of the short germ-band embryo of the flour beetle Tribolium, ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400148
更新日期:2004-06-01 00:00:00
abstract::Phosphoinositides have crucial roles in cellular controls, many of which have been established through the use of small-molecule inhibitors. Here, we describe YM201636, a potent inhibitor of the mammalian class III phosphatidylinositol phosphate kinase PIKfyve, which synthesizes phosphatidylinositol 3,5-bisphosphate. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401155
更新日期:2008-02-01 00:00:00
abstract::Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, eac...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.151
更新日期:2013-11-01 00:00:00
abstract::The orphan GluD2 receptor belongs to the ionotropic glutamate receptor family but does not bind glutamate. Ligand-gated GluD2 currents have never been evidenced, and whether GluD2 operates as an ion channel has been a long-standing question. Here, we show that GluD2 gating is triggered by type 1 metabotropic glutamate...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337371
更新日期:2014-01-01 00:00:00
abstract::Polyploidization frequently precedes tumorigenesis but also occurs during normal development in several tissues. Hepatocyte ploidy is controlled by the PIDDosome during development and regeneration. This multi-protein complex is activated by supernumerary centrosomes to induce p53 and restrict proliferation of polyplo...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202050893
更新日期:2020-11-23 00:00:00
abstract::Spatio-temporal regulation of signalling pathways plays a key role in generating diverse responses during the development of multicellular organisms. The role of signal dynamics in transferring signalling information in vivo is incompletely understood. Here, we employ genome engineering in Drosophila melanogaster to g...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201947999
更新日期:2019-12-05 00:00:00
abstract::Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201438919
更新日期:2014-08-01 00:00:00
abstract::The mitochondrial matrix protease CLPP plays a central role in the activation of the mitochondrial unfolded protein response (UPR(mt)) in Caenorhabditis elegans Far less is known about mammalian UPR(mt) signaling, although similar roles were assumed for central players, including CLPP To better understand the mammalia...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642077
更新日期:2016-07-01 00:00:00
abstract::Apoptotic cell volume decrease (AVD) and exposure of phosphatidylserine (PtdSer) at the cell surface are early events in apoptosis. However, the ion channels responsible for AVD, and their relationship to PtdSer translocation and cell death are poorly understood. Real-time analysis of calcium-induced apoptosis in lymp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor722
更新日期:2003-02-01 00:00:00
abstract::Ataxia telangiectasia mutated (ATM), the deficiency of which causes a severe neurodegenerative disease, is a crucial mediator for the DNA damage response (DDR). As neurons have high rates of transcription that require topoisomerase I (TOP1), we investigated whether TOP1 cleavage complexes (TOP1cc)-which are potent tra...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.97
更新日期:2009-08-01 00:00:00
abstract::Howy Jacobs reminisces about his many editorials and asks whether they have ever been read by anyone. ...
journal_title:EMBO reports
pub_type: 社论
doi:10.15252/embr.202050838
更新日期:2020-07-03 00:00:00
abstract::The mechanism by which YopP simultaneously inhibits mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB pathways has been elusive. Ectopic expression of YopP inhibits the activity and ubiquitination of a complex consisting of overexpressed TGF-beta-activated kinase 1 (TAK1) and its subunit TAK1-binding p...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400754
更新日期:2006-08-01 00:00:00
abstract::Cells suffer necrotic death when exposed to extreme environmental conditions, adverse and excessive stimuli, or when deleterious mutations are encoded in their genetic material. Unlike apoptosis, which involves a highly regulated and elaborate network of biochemical events and cascades, necrosis has been considered ge...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf138
更新日期:2002-07-01 00:00:00
abstract::The crucial role of ubiquitin signalling in genome-integrity maintenance was first recognized in 1987 by Stefan Jentsch and Alex Varshavsky, who showed that Rad6-the repair protein involved in DNA damage tolerance-is a ubiquitin-conjugating enzyme. Although this discovery inspired extensive research and led to the dis...
journal_title:EMBO reports
pub_type:
doi:10.1038/embor.2010.180
更新日期:2010-12-01 00:00:00
abstract::The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400773
更新日期:2006-09-01 00:00:00
abstract::The impact of the mitochondrial permeability transition (MPT) on cellular physiology is well characterized. In contrast, the composition and mode of action of the permeability transition pore complex (PTPC), the supramolecular entity that initiates MPT, remain to be elucidated. Specifically, the precise contribution o...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643602
更新日期:2017-07-01 00:00:00
abstract::Selenoprotein T (SelT) is a recently characterized thioredoxin-like protein whose expression is very high during development, but is confined to endocrine tissues in adulthood where its function is unknown. We report here that SelT is required for adaptation to the stressful conditions of high hormone level production...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643504
更新日期:2017-11-01 00:00:00
abstract::Cohesin cofactors regulate the loading, maintenance, and release of cohesin complexes from chromosomes during mitosis but little is known on their role during vertebrate meiosis. One such cofactor is PDS5, which exists as two paralogs in somatic and germline cells, PDS5A and PDS5B, with unclear functions. Here, we hav...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949273
更新日期:2020-06-04 00:00:00
abstract::Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic expression of E2F3 results in...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400452
更新日期:2005-07-01 00:00:00